JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317212

Anti-Respiratory Syncytial virus NS1 antibody [HL2383]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal NS1 antibody. Suitable for WB and reacts with Transfected cell lysate, Respiratory syncytial virus samples. Immunogen corresponding to Recombinant Fragment Protein within Human respiratory syncytial virus A (strain A2) 1C.

View Alternative Names

NS1, Non-structural protein 1, Non-structural protein 1C

2 Images
Western blot - Anti-Respiratory Syncytial virus NS1 antibody [HL2383] (AB317212)
  • WB

Supplier Data

Western blot - Anti-Respiratory Syncytial virus NS1 antibody [HL2383] (AB317212)

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 15% SDS-PAGE and the membrane was blotted with ab317212 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Respiratory Syncytial virus NS1 antibody [HL2383] (ab317212) at 1/1000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts

Lane 2:

DDDDK-tagged RSV type A NS1 transfected (+) 293T whole cell extracts

Lane 3:

DDDDK-tagged RSV type B NS1 transfected (+) 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Respiratory Syncytial virus NS1 antibody [HL2383] (AB317212)
  • WB

Supplier Data

Western blot - Anti-Respiratory Syncytial virus NS1 antibody [HL2383] (AB317212)

Non-infected (–) and infected (+) HEp-2 whole cell extracts (5 ug) were separated by 15% SDS-PAGE and the membrane was blotted with ab317212 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Respiratory Syncytial virus NS1 antibody [HL2383] (ab317212) at 1/1000 dilution

Lane 1:

Non-infected (–) HEp-2 whole cell extracts at 5 µg

Lane 2:

RSV type A infected (+) HEp-2 whole cell extracts at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2383

Isotype

IgG

Carrier free

No

Reacts with

Respiratory syncytial virus

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human respiratory syncytial virus A (strain A2) 1C.

P0DOE9

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Respiratory syncytial virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Respiratory Syncytial Virus (RSV) is a negative-sense single-stranded RNA virus known to cause respiratory infections. It belongs to the Paramyxoviridae family and is sometimes referred to by its alternate names like Human Orthopneumovirus. RSV is approximately 15 kb in size. It is predominantly expressed in the respiratory tract where it attacks epithelial cells leading to infection. The virus's ability to form a syncytium a large multinucleate cell is one of its mechanical strategies to aid in the spread across the host cells.
Biological function summary

The virus propagates efficiently within the host tissue leading to serious respiratory illness in infants and young children. RSV is not part of a specific complex but it engages host cellular machinery to promote its replication and prevent immune detection. The fusion (F) protein of RSV plays a critical role in mediating membrane fusion facilitating viral entry into the host cells. The involvement of viral proteins in modulating host immune responses is significant for the virus's life cycle.

Pathways

Respiratory infections caused by RSV connect to the host's immune signaling pathways including the toll-like receptor (TLR) pathways and the interferon response pathway. The interaction of RSV proteins with these pathways leads to an increased inflammatory response. Proteins such as the RSV G protein interface with the chemokine receptor pathway which helps in modulation of the immune system to allow viral persistence. These interactions demonstrate the virus's strategies to evade immune surveillance and promote infection.

Respiratory syncytial virus is a major cause of bronchiolitis and pneumonia in infants and young children. The virus's activity in the respiratory tract contributes to severe inflammation and cell damage which can lead to hospitalization. Other proteins like RSV M protein are involved in virus assembly and budding and they passively influence disease progression by impacting viral load and the host's immune response. Understanding these interactions is important for developing therapeutic strategies including anti-RSV monoclonal antibodies like palivizumab (2F7) which target prominent viral components to prevent severe disease outcomes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a major role in antagonizing the type I IFN-mediated antiviral response by degrading or inhibiting multiple cellular factors required for either IFN induction or response pathways (PubMed : 15047850). Acts cooperatively with NS2 to repress activation and nuclear translocation of host IFN-regulatory factor IRF3 (PubMed : 15827150). Also disrupts the association of IRF3 with CREBBP (PubMed : 21632562). Interacts with host mitochondrial-associated membrane (MAM) MAVS and prevents the interaction with RIGI (Probable) (PubMed : 22383950). Interacts with TRIM25 to suppress TRIM25-mediated RIGI ubiquitination and thereby RIGI-MAVS interaction (PubMed : 30558248). Together with NS2, participates in the proteasomal degradation of host STAT2, IRF3, IRF7, TBK1 and RIGI through a NS-degradasome involving CUL2 and Elongin-C (PubMed : 17251292, PubMed : 23877405). The degradasome requires an intact mitochondrial MAVS (PubMed : 23877405). Decreases the levels of host TRAF3 and IKBKE/IKK-epsilon (PubMed : 19625398). As functions other than disruptions of the type I IFN-mediated antiviral signaling pathways, induces host SOCS1 and SOCS3 expression (PubMed : 24480984, PubMed : 26557722). Suppresses premature apoptosis by an NF-kappa-B-dependent, interferon-independent mechanism and thus facilitates virus growth (PubMed : 17151097). Additionally, NS1 may serve some inhibitory role in viral transcription and RNA replication (PubMed : 9445048). Suppresses proliferation and activation of host CD103+ CD8+ cytotoxic T-lymphocytes and Th17 helper T-lymphocytes (PubMed : 21533073).
See full target information 1C
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com