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AB22501

Anti-Respiratory Syncytial Virus Nucleoprotein antibody [B023]

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(5 Publications)

Mouse Monoclonal NCAP antibody. Suitable for ELISA, sELISA, IHC-Fr and reacts with Respiratory syncytial virus samples. Cited in 5 publications.

View Alternative Names

Nucleoprotein, Protein N, Nucleocapsid protein, N

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B023

Isotype

IgG1

Carrier free

No

Reacts with

Respiratory syncytial virus

Applications

ELISA, sELISA, IHC-Fr

applications

Specificity

This antibody recognises the 42kD RSV nucleo protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Respiratory syncytial virus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>Use ab22501 as detection antibody</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification notes
Protein A chromatography
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Respiratory Syncytial Virus Nucleoprotein (RSV N protein) plays a central mechanical role in the replication and transcription of the RSV genome. It binds single-stranded RNA acting as a scaffold for the virus's RNA synthesis. The nucleocapsid protein another name for RSV N protein has a molecular mass of approximately 45 kilodaltons. It is expressed in cells infected by the RSV notably within the cytoplasm forming a protective helix around the viral RNA genome.
Biological function summary

Within the context of viral infection and replication RSV N protein is essential for packaging and protecting the viral RNA genome. It forms part of a complex with the phosphoprotein (P protein) and the large polymerase protein (L protein) to facilitate RNA replication and transcription. This complex ensures the stability and integrity of the viral genome allowing the virus to efficiently replicate and produce infectious particles. Therefore the nucleoprotein strongly influences the life cycle of the virus.

Pathways

The RSV N protein functional capacity is pivotal within the viral RNA transcription and replication pathways. It closely interacts with the phosphoprotein P enhancing the activity of the viral RNA-dependent RNA polymerase complex. This interaction synergistically ties into the virus's replication pathway ensuring the production of viral RNAs. The N protein also coordinates with M2-1 protein in regulating mRNA synthesis and RNA processing reflecting its comprehensive involvement in essential viral pathways.

The involvement of RSV N protein connects strongly to respiratory tract infections particularly in infants and the elderly. RSV infection often leads to bronchiolitis and pneumonia where the N protein plays an important role in propagating the disease. Its interaction with the fusion glycoprotein (F protein) facilitates virus assembly and release which enhances viral spread and subsequent infection severity. Researchers often focus on these proteins when developing therapeutics targeting RSV-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases (PubMed : 19965480, PubMed : 23677789, PubMed : 31229488). RNA replication depends on the availability of soluble nucleoprotein (PubMed : 22623798, PubMed : 9299631). The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication (PubMed : 22623798). Together with the phosphoprotein, sequesters host NF-kappa-B in inclusion bodies (IBs) thereby inhibiting this host defense pathway (PubMed : 32878896). May also act as a modulator of the innate immune response by sequestration of host IFIH1/MDA5 and MAVS into IBs (PubMed : 22623778).
See full target information N
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature microbiology 7:1879-1890 PubMed36280786

2022

Coinfection by influenza A virus and respiratory syncytial virus produces hybrid virus particles.

Applications

Unspecified application

Species

Unspecified reactive species

Joanne Haney,Swetha Vijayakrishnan,James Streetley,Kieran Dee,Daniel Max Goldfarb,Mairi Clarke,Margaret Mullin,Stephen D Carter,David Bhella,Pablo R Murcia

Nature communications 9:3999 PubMed30275522

2018

Engineered mRNA-expressed antibodies prevent respiratory syncytial virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Pooja Munnilal Tiwari,Daryll Vanover,Kevin E Lindsay,Swapnil Subhash Bawage,Jonathan L Kirschman,Sushma Bhosle,Aaron W Lifland,Chiara Zurla,Philip J Santangelo

Nature communications 8:667 PubMed28939853

2017

RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Daryll Vanover,Daisy V Smith,Emmeline L Blanchard,Eric Alonas,Jonathan L Kirschman,Aaron W Lifland,Chiara Zurla,Philip J Santangelo

Light, science & applications 5: PubMed27398242

2016

Mirror-enhanced super-resolution microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Xusan Yang,Hao Xie,Eric Alonas,Yujia Liu,Xuanze Chen,Philip J Santangelo,Qiushi Ren,Peng Xi,Dayong Jin

Journal of medical primatology 41:403-6 PubMed22931057

2012

Respiratory syncytial virus (RSV) pneumonia in a southern muriqui (Brachyteles arachnoides).

Applications

Unspecified application

Species

Unspecified reactive species

S V Santos,R F Strefezzi,A Pissinatti,C F H Takakura,C Kanamura,M I S Duarte,J L Catão-Dias
View all publications
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