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AB308394

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1246]

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Rabbit Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line, Transfected cell line - Respiratory syncytial virus samples.

View Alternative Names

Nucleoprotein, Respiratory syncytial virus nucleoprotein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (AB308394)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (AB308394)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Respiratory Syncytial virus Nucleoprotein using ab308394 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Western blot - Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (AB308394)
  • WB

Supplier Data

Western blot - Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (AB308394)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (ab308394) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (ab308394) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (AB308394)
  • WB

Supplier Data

Western blot - Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (AB308394)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (ab308394) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (ab308394) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1246

Isotype

IgG

Carrier free

Yes

Reacts with

Respiratory syncytial virus

Applications

ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Respiratory syncytial virus": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Respiratory syncytial virus": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Respiratory Syncytial Virus Nucleoprotein (RSV N protein) plays a central mechanical role in the replication and transcription of the RSV genome. It binds single-stranded RNA acting as a scaffold for the virus's RNA synthesis. The nucleocapsid protein another name for RSV N protein has a molecular mass of approximately 45 kilodaltons. It is expressed in cells infected by the RSV notably within the cytoplasm forming a protective helix around the viral RNA genome.
Biological function summary

Within the context of viral infection and replication RSV N protein is essential for packaging and protecting the viral RNA genome. It forms part of a complex with the phosphoprotein (P protein) and the large polymerase protein (L protein) to facilitate RNA replication and transcription. This complex ensures the stability and integrity of the viral genome allowing the virus to efficiently replicate and produce infectious particles. Therefore the nucleoprotein strongly influences the life cycle of the virus.

Pathways

The RSV N protein functional capacity is pivotal within the viral RNA transcription and replication pathways. It closely interacts with the phosphoprotein P enhancing the activity of the viral RNA-dependent RNA polymerase complex. This interaction synergistically ties into the virus's replication pathway ensuring the production of viral RNAs. The N protein also coordinates with M2-1 protein in regulating mRNA synthesis and RNA processing reflecting its comprehensive involvement in essential viral pathways.

The involvement of RSV N protein connects strongly to respiratory tract infections particularly in infants and the elderly. RSV infection often leads to bronchiolitis and pneumonia where the N protein plays an important role in propagating the disease. Its interaction with the fusion glycoprotein (F protein) facilitates virus assembly and release which enhances viral spread and subsequent infection severity. Researchers often focus on these proteins when developing therapeutics targeting RSV-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Respiratory Syncytial Virus Nucleoprotein
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com