Rabbit Recombinant Monoclonal REST / NRSF antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (AB315008), expandable thumbnail](https://content.abcam.com/products/images/rest-nrsf-antibody-epr24988-29-bsa-and-azide-free-ab315008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432433.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (AB315008)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (AB315008), expandable thumbnail](https://content.abcam.com/products/images/rest-nrsf-antibody-epr24988-29-bsa-and-azide-free-ab315008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432434.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (AB315008)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (AB315008), expandable thumbnail](https://content.abcam.com/products/images/rest-nrsf-antibody-epr24988-29-bsa-and-azide-free-ab315008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432435.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (AB315008)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (AB315008), expandable thumbnail](https://content.abcam.com/products/images/rest-nrsf-antibody-epr24988-29-bsa-and-azide-free-ab315008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432436.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (AB315008)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt (Intra) | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells (PubMed:29961578, PubMed:9771705). Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes (By similarity). Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier (By similarity). Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression (By similarity). Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural-specific splicing events and to prevent production of REST isoform 2 (PubMed:21884984). Repressor activity may be inhibited by forming heterodimers with isoform 2, thereby preventing binding to NRSE or binding to corepressors and leading to derepression of target genes (PubMed:10490617, PubMed:11039732). Also maintains repression of neuronal genes in neural stem cells, and allows transcription and differentiation into neurons by dissociation from RE1/NRSE sites of target genes (PubMed:15907476). Thereby is involved in maintaining the quiescent state of adult neural stem cells and preventing premature differentiation into mature neurons (PubMed:27819263). Plays a role in the developmental switch in synaptic NMDA receptor composition during postnatal development, by repressing GRIN2B expression and thereby altering NMDA receptor properties from containing primarily GRIN2B to primarily GRIN2A subunits (By similarity). Acts as a regulator of osteoblast differentiation (PubMed:25727884). Key repressor of gene expression in hypoxia; represses genes in hypoxia by direct binding to an RE1/NRSE site on their promoter regions (By similarity). May also function in stress resistance in the brain during aging; possibly by regulating expression of genes involved in cell death and in the stress response (PubMed:24670762). Repressor of gene expression in the hippocampus after ischemia by directly binding to RE1/NRSE sites and recruiting SIN3A and RCOR1 to promoters of target genes, thereby promoting changes in chromatin modifications and ischemia-induced cell death (By similarity). After ischemia, might play a role in repression of miR-132 expression in hippocampal neurons, thereby leading to neuronal cell death (By similarity). Isoform 2. Binds to the 3' region of the neuron-restrictive silencer element (NRSE), with lower affinity than isoform 1 (PubMed:11039732). Exhibits weaker repressor activity compared to isoform 1 (By similarity). May negatively regulate the repressor activity of isoform 1 by binding to isoform 1, thereby preventing its binding to NRSE and leading to derepression of target genes (PubMed:10490617, PubMed:11039732). However, in another study, does not appear to be implicated in repressor activity of a NRSE motif-containing reporter construct nor in inhibitory activity on the isoform 1 transcriptional repressor activity (By similarity). Post-transcriptional inactivation of REST by SRRM4-dependent alternative splicing into isoform 2 is required in mechanosensory hair cells in the inner ear for derepression of neuronal genes, maintenance of hair cells and hearing (PubMed:29961578).
Alternative names
Nrsf, Rest, RE1-silencing transcription factor, Neural-restrictive silencer factor
Recommended products
Rabbit Recombinant Monoclonal REST / NRSF antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24988-29
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab315008 is the carrier-free version of Anti-REST / NRSF antibody [EPR24988-29] ab315007.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
REST also known as NRSF (neuron-restrictive silencer factor) is a transcriptional repressor weighing approximately 116 kDa. It regulates gene expression by binding to the RE1 silencing transcription factor (RE1-silencing element) in the DNA. REST is widely expressed in non-neuronal cells and plays a significant role in maintaining neuronal gene silencing in embryonic and adult stem cells. By interacting with co-repressors REST recruits histone deacetylases and methylases to modify chromatin structure effectively shutting down gene transcription in specific contexts.
- Biological function summary
REST regulates neuronal differentiation and plasticity by controlling the expression of neuronal-specific genes. It forms a complex with co-repressors such as mSin3 and CoREST participating in diverse biological processes including neurogenesis. REST expression is important for balancing the neuronal gene expression program preventing untimely or inappropriate activation of neuronal genes in non-neuronal tissues. By modulating the expression of synaptic proteins and neurotransmitter receptors REST influences synaptic plasticity and neuronal excitability.
- Pathways
REST participates in the neural stem cell differentiation pathway and cell fate determination. It interacts with pathways involving proteins such as HDAC1 and SWI/SNF complexes contributing to chromatin remodeling and transcriptional regulation. Within these pathways REST ensures the maintenance of non-neuronal identity by repressing neuronal gene activation coordinating with other proteins to influence cellular outcomes and fate decisions.
- Associated diseases and disorders
REST has been linked to neurological disorders such as Huntington's disease and epilepsy. In Huntington's disease REST dissociation from the Huntingtin protein increases leading to misregulation of neuronal genes and contributing to neurodegenerative changes. REST also plays a role in epilepsy where its dysregulation can lead to an imbalance in excitatory and inhibitory signals within the brain potentially triggering epileptic episodes. Through its interactions with disease-associated proteins REST exemplifies the complex interplay between transcriptional regulation and cellular dysfunction in disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008), expandable thumbnail](https://content.abcam.com/products/images/rest-nrsf-antibody-epr24988-29-bsa-and-azide-free-ab315008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432433.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008)
This data was developed using Anti-REST / NRSF antibody [EPR24988-29] ab315007, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling REST / NRSF with Anti-REST / NRSF antibody [EPR24988-29] ab315007 at 1/500 (1.048 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Nuclear staining on human colon.
The section was incubated with Anti-REST / NRSF antibody [EPR24988-29] ab315007 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008), expandable thumbnail](https://content.abcam.com/products/images/rest-nrsf-antibody-epr24988-29-bsa-and-azide-free-ab315008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432434.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008)
This data was developed using Anti-REST / NRSF antibody [EPR24988-29] ab315007, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling REST / NRSF with Anti-REST / NRSF antibody [EPR24988-29] ab315007 at 1/500 (1.048 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Nuclear staining on human kidney (PMID: 29961578).
The section was incubated with Anti-REST / NRSF antibody [EPR24988-29] ab315007 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008), expandable thumbnail](https://content.abcam.com/products/images/rest-nrsf-antibody-epr24988-29-bsa-and-azide-free-ab315008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432435.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008)
This data was developed using Anti-REST / NRSF antibody [EPR24988-29] ab315007, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling REST / NRSF with Anti-REST / NRSF antibody [EPR24988-29] ab315007 at 1/500 (1.048 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Nuclear staining on human spleen (PMID: 29961578).
The section was incubated with Anti-REST / NRSF antibody [EPR24988-29] ab315007 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008), expandable thumbnail](https://content.abcam.com/products/images/rest-nrsf-antibody-epr24988-29-bsa-and-azide-free-ab315008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432436.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody [EPR24988-29] - BSA and Azide free (ab315008)
This data was developed using Anti-REST / NRSF antibody [EPR24988-29] ab315007, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) SH-SY5Y (Human neuroblastoma epithelial cell) (Untreated), (B) SH-SY5Y (16 nM 48h) (TPA treated). tissue labeling REST / NRSF with Anti-REST / NRSF antibody [EPR24988-29] ab315007 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) untreated SH-SY5Y, and the expression of (B) SH-SY5Y (16 nM 48h) (TPA treated) decreased (PMID: 25093670).
The section was incubated with Anti-REST / NRSF antibody [EPR24988-29] ab315007 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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