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AB8957

Anti-Reticulon 1A antibody [MON160]

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(3 Publications)

Mouse Monoclonal RTN1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RTN1 aa 1 to C-terminus.

View Alternative Names

NSP, RTN1, Reticulon-1, Neuroendocrine-specific protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (AB8957)
  • IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (AB8957)

NSP-A immunostaining in human cerebellum using ab8957.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (AB8957)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (AB8957)

Human carcinoid (A) and small cell lung carcinoma (B) in paraffin sections, immunostained for NSP-A with ab8957.

Schematic Diagram - Anti-Reticulon 1A antibody [MON160] (AB8957)
  • Schematic Diagram

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Schematic Diagram - Anti-Reticulon 1A antibody [MON160] (AB8957)

Schematic representation of of the deduced domain structure of the three NSP-gene encoded proteins, NSP-A, NSP-B and NSP-C and mapping of the epitopes recognized by the different NSP monoclonal antibodies.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MON160

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RTN1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16799

Epitope

This antibody is reactive with residues 174-337 of Reticulon-1A.

Specificity

This antibody exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.</p>" } } }
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Product details

A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Reticulon 1A also known as neuroendocrine-specific protein (NSP) is part of the reticulon family of proteins. It is a membrane protein with a mass of approximately 135 kDa. This protein is found primarily in the endoplasmic reticulum (ER) where it participates in the maintenance of ER structure. Reticulon 1A is expressed in neuronal cells as well as in peripheral tissues. It plays a role in shaping the tubular endoplasmic reticulum which is important for its function within the cell.
Biological function summary

The reticulon family members including Reticulon 1A influence membrane dynamics and participate in various cellular processes. Reticulon 1A is not typically associated with any specific protein complex but interacts with other ER-shaping proteins to help maintain the network of the ER. This interaction allows it to participate in lipid biosynthesis and protein processing which are both important for maintaining cellular homeostasis.

Pathways

Reticulon 1A interacts with several other proteins that are involved in the regulation of ER morphology. It plays a role in the ER stress response pathway which involves proteins such as BiP and IRE1. Additionally Reticulon 1A has a role in apoptosis pathways particularly in stress conditions when misfolded proteins accumulate in the ER.

Reticulon 1A has links to neurodegenerative diseases and certain forms of cancer. In the context of neurodegeneration altered expression of Reticulon 1A has been observed in Alzheimer's disease where it interacts with amyloid precursor protein (APP). In cancer Reticulon 1A expression can influence tumor growth by affecting cellular proliferation and apoptosis making it a potential target for therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits amyloid precursor protein processing, probably by blocking BACE1 activity.
See full target information RTN1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Society of Nephrology : JA 28:2007-2021 PubMed28137829

2017

Inhibition of Reticulon-1A-Mediated Endoplasmic Reticulum Stress in Early AKI Attenuates Renal Fibrosis Development.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Fan,Wenzhen Xiao,Kyung Lee,Fadi Salem,Jiejun Wen,Li He,Jing Zhang,Yang Fei,Dongsheng Cheng,Hongda Bao,Yumei Liu,Fujun Lin,Gengru Jiang,Zhiyong Guo,Niansong Wang,John Cijiang He

American journal of physiology. Renal physiology 310:F409-15 PubMed26739891

2016

Knockdown of RTN1A attenuates ER stress and kidney injury in albumin overload-induced nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzhen Xiao,Ying Fan,Niansong Wang,Peter Y Chuang,Kyung Lee,John Cijiang He

Nature communications 6:7841 PubMed26227493

2015

RTN1 mediates progression of kidney disease by inducing ER stress.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Fan,Wenzhen Xiao,Zhengzhe Li,Xuezhu Li,Peter Y Chuang,Belinda Jim,Weijia Zhang,Chengguo Wei,Niansong Wang,Weiping Jia,Huabao Xiong,Kyung Lee,John C He
View all publications
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