AB9274

Anti-Reticulon 1A antibody [MON162]

Name: Anti-Reticulon 1A antibody [MON162] (ab9274) | Abcam
Brand: Abcam Limited
SKU: AB9274
Price: 535 USD
Availability: InStock

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(12 Publications)

Mouse Monoclonal RTN1 antibody. Suitable for ICC/IF, IHC-Fr and reacts with Human, Pig samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RTN1 aa 1 to C-terminus.

View Alternative Names

NSP, RTN1, Reticulon-1, Neuroendocrine-specific protein

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (AB9274)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (AB9274)

Immunostaining of NCI-H82 (a human cell line) variant small cell lung cancer

Immunohistochemistry (Frozen sections) - Anti-Reticulon 1A antibody [MON162] (AB9274)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Reticulon 1A antibody [MON162] (AB9274)

Immunohistochemistry on frozen section of swine brain

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MON162

Isotype

IgG1

Carrier free

Reacts with

Human, Pig

Applications

ICC/IF, IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Human RTN1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16799

Epitope

The epitope of this antibody is located between amino acid residues 338-422 of Reticulon-1A.

Specificity

This antibody exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues

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Reactivity data

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Product details

A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Reticulon 1A also known as neuroendocrine-specific protein (NSP) is part of the reticulon family of proteins. It is a membrane protein with a mass of approximately 135 kDa. This protein is found primarily in the endoplasmic reticulum (ER) where it participates in the maintenance of ER structure. Reticulon 1A is expressed in neuronal cells as well as in peripheral tissues. It plays a role in shaping the tubular endoplasmic reticulum which is important for its function within the cell.

Biological function summary

The reticulon family members including Reticulon 1A influence membrane dynamics and participate in various cellular processes. Reticulon 1A is not typically associated with any specific protein complex but interacts with other ER-shaping proteins to help maintain the network of the ER. This interaction allows it to participate in lipid biosynthesis and protein processing which are both important for maintaining cellular homeostasis.

Pathways

Reticulon 1A interacts with several other proteins that are involved in the regulation of ER morphology. It plays a role in the ER stress response pathway which involves proteins such as BiP and IRE1. Additionally Reticulon 1A has a role in apoptosis pathways particularly in stress conditions when misfolded proteins accumulate in the ER.

Reticulon 1A has links to neurodegenerative diseases and certain forms of cancer. In the context of neurodegeneration altered expression of Reticulon 1A has been observed in Alzheimer's disease where it interacts with amyloid precursor protein (APP). In cancer Reticulon 1A expression can influence tumor growth by affecting cellular proliferation and apoptosis making it a potential target for therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits amyloid precursor protein processing, probably by blocking BACE1 activity.

See full target information RTN1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed39704174

2024

RTN1A mediates diabetes-induced AKI-to-CKD transition.

Applications

Unspecified application

Species

Unspecified reactive species

Lulin Min,Ya Chen,Yixin Chen,Fang Zhong,Zhaohui Ni,Leyi Gu,Kyung Lee,John Cijiang He

Nature 618:402-410 PubMed37225994

2023

Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy.

Applications

Unspecified application

Species

Unspecified reactive species

Hector Foronda,Yangxue Fu,Adriana Covarrubias-Pinto,Hartmut T Bocker,Alexis González,Eric Seemann,Patricia Franzka,Andrea Bock,Ramachandra M Bhaskara,Lutz Liebmann,Marina E Hoffmann,Istvan Katona,Nicole Koch,Joachim Weis,Ingo Kurth,Joseph G Gleeson,Fulvio Reggiori,Gerhard Hummer,Michael M Kessels,Britta Qualmann,Muriel Mari,Ivan Dikić,Christian A Hübner

eLife 11: PubMed36223176

2022

Interoperability of RTN1A in dendrite dynamics and immune functions in human Langerhans cells.

Applications

Unspecified application

Species

Unspecified reactive species

Małgorzata Anna Cichoń,Karin Pfisterer,Judith Leitner,Lena Wagner,Clement Staud,Peter Steinberger,Adelheid Elbe-Bürger

Cancer science 109:2469-2478 PubMed29890027

2018

Tumor necrosis factor superfamily 15 promotes lymphatic metastasis via upregulation of vascular endothelial growth factor-C in a mouse model of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Qin,Dingzhi Huang,Zhujun Liu,Xiaoling Zhang,Yanan Jia,Cory J Xian,Kai Li

Journal of immunology (Baltimore, Md. : 1950) 200:260-270 PubMed29167231

2017

Reticulon-1 and Reduced Migration toward Chemoattractants by Macrophages Differentiated from the Bone Marrow of Ultraviolet-Irradiated and Ultraviolet-Chimeric Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Terence A McGonigle,Amy R Dwyer,Eloise L Greenland,Naomi M Scott,Kim W Carter,Kevin N Keane,Philip Newsholme,Helen S Goodridge,Fiona J Pixley,Prue H Hart

eLife 6: PubMed28617241

2017

Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.

Applications

Species

Unspecified reactive species

Paolo Grumati,Giulio Morozzi,Soraya Hölper,Muriel Mari,Marie-Lena Ie Harwardt,Riqiang Yan,Stefan Müller,Fulvio Reggiori,Mike Heilemann,Ivan Dikic

PloS one 9:e97306 PubMed24845259

2014

Normal fibroblasts induce E-cadherin loss and increase lymph node metastasis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Xu,Xinlei Hu,Zhongting Chen,Xiaoping Zheng,Chenjing Zhang,Gang Wang,Yu Chen,Xinglu Zhou,Xiaoxiao Tang,Laisheng Luo,Xiang Xu,Wensheng Pan

Cell and tissue research 292:229-37 PubMed9560466

1998

Neuronal differentiation is accompanied by NSP-C expression.

Applications

Unspecified application

Species

Unspecified reactive species

J Hens,R Nuydens,H Geerts,N H Senden,W J Van de Ven,A J Roebroek,H J van de Velde,F C Ramaekers,J L Broers

The Journal of pathology 182:13-21 PubMed9227337

1997

A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers.

Applications

Unspecified application

Species

Unspecified reactive species

N H Senden,E D Timmer,A de Bruïne,S S Wagenaar,H J Van de Velde,A J Roebroek,W J Van de Ven,J L Broers,F C Ramaekers

Cancer research 54:4769-76 PubMed8062278

1994

NSP-encoded reticulons are neuroendocrine markers of a novel category in human lung cancer diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

H J van de Velde,N H Senden,T A Roskams,J L Broers,F C Ramaekers,A J Roebroek,W J Van de Ven

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Reticulon 1A antibody [MON162]

Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (AB9274)

Immunohistochemistry (Frozen sections) - Anti-Reticulon 1A antibody [MON162] (AB9274)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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