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AB237514

Anti-Retinoblastoma binding protein 6 antibody

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(6 Publications)

Rabbit Polyclonal Retinoblastoma binding protein 6 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBBP6 aa 50-100.

View Alternative Names

P2PR, PACT, RBQ1, My038, RBBP6, E3 ubiquitin-protein ligase RBBP6, Proliferation potential-related protein, Protein P2P-R, RING-type E3 ubiquitin transferase RBBP6, Retinoblastoma-binding Q protein 1, Retinoblastoma-binding protein 6, p53-associated cellular protein of testis, RBQ-1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoblastoma binding protein 6 antibody (AB237514)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoblastoma binding protein 6 antibody (AB237514)

Paraffin-embedded human testis tissue stained for Retinoblastoma binding protein 6 using ab237514 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoblastoma binding protein 6 antibody (AB237514)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoblastoma binding protein 6 antibody (AB237514)

Paraffin-embedded human cervical cancer tissue stained for Retinoblastoma binding protein 6 using ab237514 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Retinoblastoma binding protein 6 antibody (AB237514)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Retinoblastoma binding protein 6 antibody (AB237514)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Retinoblastoma binding protein 6 (green) using ab237514 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-Retinoblastoma binding protein 6 antibody (AB237514)
  • WB

Supplier Data

Western blot - Anti-Retinoblastoma binding protein 6 antibody (AB237514)

All lanes:

Western blot - Anti-Retinoblastoma binding protein 6 antibody (ab237514) at 1/500 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 202 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RBBP6 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q7Z6E9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The retinoblastoma-binding protein 6 (RBBP6) also known as P2 is a multifunctional protein with a molecular mass of approximately 200 kDa. RBBP6 interacts with various proteins such as p53 and the retinoblastoma protein (Rb). It participates in processes like transcription splicing and ubiquitination. Cells express RBBP6 in tissues across multiple organs with significant levels present in the liver heart and lung tissues. The expression pattern indicates a wide role in cellular functions.
Biological function summary

RBBP6 functions as part of a complex involved in cell cycle regulation and apoptosis. It interacts with the p53 tumor suppressor protein facilitating its regulation and influences various cellular processes. Additionally RBBP6 is implicated in RNA processing due to its association with the 3'-end processing of mRNA. Through these roles RBBP6 ensures proper cellular response to stress and DNA damage connecting it to mechanisms of maintaining genomic stability.

Pathways

RBBP6 plays a significant role in the p53 signaling and ubiquitin-proteasome pathways. Within the p53 pathway RBBP6 modulates p53 activity by affecting its degradation; this interaction impacts cell cycle arrest and apoptosis. It also contributes to the ubiquitin-proteasome system by labeling proteins for degradation which maintains protein quality and turnover. These pathways show RBBP6's pivotal function in controlling cell proliferation and survival.

Anomalies in RBBP6 expression or function link to cancer and retinoblastoma. Mutations in RBBP6 can disrupt its interaction with p53 and Rb proteins leading to uncontrolled cell proliferation and tumorigenesis. The protein's dysfunction may also contribute to the development of retinoblastoma a pediatric eye cancer as improper Rb protein regulation compromises cell cycle control. Understanding RBBP6’s role in these diseases offers potential for targeted therapies in oncology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome (PubMed : 18851979). May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth (By similarity). Regulates DNA-replication and the stability of chromosomal common fragile sites (CFSs) in a ZBTB38- and MCM10-dependent manner. Controls ZBTB38 protein stability and abundance via ubiquitination and proteasomal degradation, and ZBTB38 in turn negatively regulates the expression of MCM10 which plays an important role in DNA-replication (PubMed : 24726359).. (Microbial infection) [Isoform 1] : Restricts ebolavirus replication probably by impairing the vp30-NP interaction, and thus viral transcription.
See full target information RBBP6
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed39857778

2025

Ezetimibe Anticancer Activity via the p53/Mdm2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Charmy Twala,Sibusiso Malindisa,Chamone Munnik,Selisha Sooklal,Monde Ntwasa

Molecular and cellular biochemistry 479:929-940 PubMed37256445

2023

miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Dong,Jin Chen,Yadong Wang,Junfei Weng,Xingxiang Du,Xu Fang,Wenyu Liu,Tao Long,Jiaxiang You,Wensheng Wang,Xiaoping Peng

British journal of cancer 128:1086-1094 PubMed36593359

2023

Dysregulation of alternative splicing contributes to multiple myeloma pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Song,Weimin Zhang,Qi Li,Zhiming Wang,Qi Su,Xinyun Zhang,Bingzong Li,Wenzhuo Zhuang

Molecular medicine reports 24: PubMed34132380

2021

MicroRNA‑193a‑5p exerts a tumor suppressive role in epithelial ovarian cancer by modulating RBBP6.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangli Zhang,Jun Liu,Jie He,Nuo Yi

International journal of laboratory hematology 43:1050-1061 PubMed33683013

2021

The lncRNA SLCO4A1-AS1/miR-876-3p/RBBP6 axis regulates cell proliferation and apoptosis in acute lymphocytic leukemia via the JNK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianping Mao,Wenliang Gao,Lianguo Xue,Juan Wang,Lidong Zhao

Cell biology international 44:2107-2119 PubMed32662898

2020

Analysis of the expression and association of retinoblastoma binding protein 6 with the JNK signaling pathway in prostate cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Ying Guan,Sheng Zhao,Yun-Na Luo
View all publications
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