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Rabbit Recombinant Monoclonal Retinoic Acid Receptor alpha antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 2 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Tested
Not recommended
Tested
Tested
Mouse
Not recommended
Tested
Not recommended
Tested
Tested
Rat
Not recommended
Expected
Not recommended
Tested
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Receptor for retinoic acid (PubMed:19850744, PubMed:16417524, PubMed:20215566). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes (PubMed:28167758). The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5 (PubMed:28167758). In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone deacetylation, chromatin condensation and transcriptional suppression (PubMed:16417524). On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation (PubMed:9267036, PubMed:19850744, PubMed:20215566). Formation of a complex with histone deacetylases might lead to inhibition of RARE DNA element binding and to transcriptional repression (PubMed:28167758). Transcriptional activation and RARE DNA element binding might be supported by the transcription factor KLF2 (PubMed:28167758). RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis (By similarity). Has a role in the survival of early spermatocytes at the beginning prophase of meiosis (By similarity). In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes (By similarity). In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Together with RXRA, positively regulates microRNA-10a expression, thereby inhibiting the GATA6/VCAM1 signaling response to pulsatile shear stress in vascular endothelial cells (PubMed:28167758). In association with HDAC3, HDAC5 and HDAC7 corepressors, plays a role in the repression of microRNA-10a and thereby promotes the inflammatory response (PubMed:28167758).

Alternative names

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Rabbit Recombinant Monoclonal Retinoic Acid Receptor alpha antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23871-271

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

RARA plays an essential role in mediating the effects of retinoic acid in the body. It is part of a larger receptor complex that interacts with co-regulators to modulate gene expression. This process is significant for embryonic development and the maintenance of normal physiological functions. Through its action RARA contributes to the proper development of organs and is critical for maintaining immune homeostasis and enabling the cellular response to environmental changes.

Activity summary

Retinoic Acid Receptor alpha (RARA) a member of the nuclear receptor superfamily functions as a transcription factor activated by retinoic acid. Commonly referenced in literature RARA has an approximate molecular mass of 50 kDa. It is expressed in a variety of tissues including the liver the lung and the immune system. By forming heterodimers with retinoid X receptors RARA regulates the transcription of genes linked to cell differentiation proliferation and apoptosis.

Pathways

RARA's activity impacts important signaling routes such as the retinoic acid signaling pathway and the Wnt signaling pathway. It collaborates with proteins like retinoid X receptors (RXRs) and other nuclear receptors to influence gene expression processes. These pathways maintain cellular differentiation and tissue homeostasis demonstrating RARA's integrative role in cellular signaling and communication.

Associated diseases and disorders

RARA's dysregulation has been linked to acute promyelocytic leukemia (APL) and some autoimmune diseases. In APL aberrant fusion proteins involving RARA disrupt normal transcriptional regulation leading to malignant transformation. Additionally the interaction of RARA with proteins such as promyelocytic leukemia protein (PML) further influences the disease's development. Research on these associations highlights the therapeutic potential of targeting RARA activities for disease intervention and treatment strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745), expandable thumbnail

    Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745)

    Lanes 1 - 4: Merged signal (red and green). Green - ab275745 observed at 40 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab275745 was shown to react with Retinoic Acid Receptor alpha in wild-type HeLa cells in western blot. The bands observed in RARA knockout cell line ab265176 (RARA knockout cell lysate ab257629) below 40 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and RARA CRISPR/Cas9 HeLa edited cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab275745 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: RARA CRISPR-Cas9 edited HeLa cell lysate at 20 µg

    Lane 3: MCF7 cell lysate at 20 µg

    Lane 4: HepG2 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 51 kDa

    Observed band size: 50-55 kDa

  • Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745), expandable thumbnail

    Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: human brain, human skeletal muscle (Database:GTExPortal, HPA).

    Exposure time: 103 seconds.

    All lanes: Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Human brain tissue lysate at 20 µg

    Lane 3: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 51 kDa

    Observed band size: 52 kDa

  • Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745), expandable thumbnail

    Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745)

    Retinoic Acid Receptor alpha was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab275745 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275745 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 ug

    Lane 2: ab275745 IP in HEK-293 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275745 in HEK-293 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 84 seconds.

    All lanes: Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745)

    Predicted band size: 51 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745), expandable thumbnail

    Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 70 seconds.

    All lanes: Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa

    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Retinoic Acid Receptor alpha with ab275745 at 1/1000 (2.71 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on sertoli cells of human testis. The section was incubated with ab275745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745), expandable thumbnail

    Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745)

    Retinoic Acid Receptor alpha was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab275745 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275745 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug

    Lane 2: ab275745 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275745 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 84 seconds.

    All lanes: Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745)

    Predicted band size: 51 kDa

    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Retinoic Acid Receptor alpha with ab275745 at 1/1000 (0.542 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on sertoli cells of rat testis. The section was incubated with ab275745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745), expandable thumbnail

    Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: MOLT-4.(Database:HPA).

    Exposure time: Lane 1: 8 seconds Lane2-4: 70 seconds.

    All lanes: Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

    Lane 3: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 4: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa

    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Retinoic Acid Receptor alpha with ab275745 at 1/1000 (0.542 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on sertoli cells of mouse testis (PMID:16210368). The section was incubated with ab275745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745), expandable thumbnail

    Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745)

    Anti-RARA antibody [EPR23871-271] (ab275745) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab275745 was shown to bind specifically to RARA. A band was observed at 50-55 kDa in wild-type MCF7 cell lysates with no signal observed at this size in RARA knockout cell line ab277158 (knockout cell lysate ab281383). To generate this image, wild-type and RARA knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745) at 1/1000 dilution

    Lane 1: Wild-type MCF7 cell lysate at 20 µg

    Lane 2: RARA knockout MCF7 cell lysate at 20 µg

    Lane 3: Human Testis cell lysate at 20 µg

    Lane 4: Human Brain cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 51 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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