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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal Retinoic Acid Receptor alpha antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 2 publications.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ICC/IF | IP | Flow Cyt | WB | IHC-P | |
---|---|---|---|---|---|
Human | Not recommended | Tested | Not recommended | Tested | Tested |
Mouse | Not recommended | Tested | Not recommended | Tested | Tested |
Rat | Not recommended | Expected | Not recommended | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Receptor for retinoic acid (PubMed:19850744, PubMed:16417524, PubMed:20215566). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes (PubMed:28167758). The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5 (PubMed:28167758). In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone deacetylation, chromatin condensation and transcriptional suppression (PubMed:16417524). On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation (PubMed:9267036, PubMed:19850744, PubMed:20215566). Formation of a complex with histone deacetylases might lead to inhibition of RARE DNA element binding and to transcriptional repression (PubMed:28167758). Transcriptional activation and RARE DNA element binding might be supported by the transcription factor KLF2 (PubMed:28167758). RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis (By similarity). Has a role in the survival of early spermatocytes at the beginning prophase of meiosis (By similarity). In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes (By similarity). In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Together with RXRA, positively regulates microRNA-10a expression, thereby inhibiting the GATA6/VCAM1 signaling response to pulsatile shear stress in vascular endothelial cells (PubMed:28167758). In association with HDAC3, HDAC5 and HDAC7 corepressors, plays a role in the repression of microRNA-10a and thereby promotes the inflammatory response (PubMed:28167758).
Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1, NR1B1, RARA
Rabbit Recombinant Monoclonal Retinoic Acid Receptor alpha antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 2 publications.
Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1, NR1B1, RARA
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR23871-271
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
RARA plays an essential role in mediating the effects of retinoic acid in the body. It is part of a larger receptor complex that interacts with co-regulators to modulate gene expression. This process is significant for embryonic development and the maintenance of normal physiological functions. Through its action RARA contributes to the proper development of organs and is critical for maintaining immune homeostasis and enabling the cellular response to environmental changes.
Retinoic Acid Receptor alpha (RARA) a member of the nuclear receptor superfamily functions as a transcription factor activated by retinoic acid. Commonly referenced in literature RARA has an approximate molecular mass of 50 kDa. It is expressed in a variety of tissues including the liver the lung and the immune system. By forming heterodimers with retinoid X receptors RARA regulates the transcription of genes linked to cell differentiation proliferation and apoptosis.
RARA's activity impacts important signaling routes such as the retinoic acid signaling pathway and the Wnt signaling pathway. It collaborates with proteins like retinoid X receptors (RXRs) and other nuclear receptors to influence gene expression processes. These pathways maintain cellular differentiation and tissue homeostasis demonstrating RARA's integrative role in cellular signaling and communication.
RARA's dysregulation has been linked to acute promyelocytic leukemia (APL) and some autoimmune diseases. In APL aberrant fusion proteins involving RARA disrupt normal transcriptional regulation leading to malignant transformation. Additionally the interaction of RARA with proteins such as promyelocytic leukemia protein (PML) further influences the disease's development. Research on these associations highlights the therapeutic potential of targeting RARA activities for disease intervention and treatment strategies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Lanes 1 - 4: Merged signal (red and green). Green - ab275745 observed at 40 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab275745 was shown to react with Retinoic Acid Receptor alpha in wild-type HeLa cells in western blot. The bands observed in RARA knockout cell line ab265176 (RARA knockout cell lysate ab257629) below 40 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and RARA CRISPR/Cas9 HeLa edited cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab275745 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: RARA CRISPR-Cas9 edited HeLa cell lysate at 20 µg
Lane 3: MCF7 cell lysate at 20 µg
Lane 4: HepG2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 50-55 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: human brain, human skeletal muscle (Database:GTExPortal, HPA).
Exposure time: 103 seconds.
All lanes: Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Human brain tissue lysate at 20 µg
Lane 3: Human skeletal muscle tissue lysate at 20 µg
All lanes: VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 52 kDa
Retinoic Acid Receptor alpha was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab275745 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275745 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 ug
Lane 2: ab275745 IP in HEK-293 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275745 in HEK-293 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 84 seconds.
All lanes: Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745)
Predicted band size: 51 kDa
Observed band size: 52 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 70 seconds.
All lanes: Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 52 kDa
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Retinoic Acid Receptor alpha with ab275745 at 1/1000 (2.71 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on sertoli cells of human testis. The section was incubated with ab275745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Retinoic Acid Receptor alpha was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab275745 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275745 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug
Lane 2: ab275745 IP in NIH/3T3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275745 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 84 seconds.
All lanes: Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745)
Predicted band size: 51 kDa
Observed band size: 52 kDa
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Retinoic Acid Receptor alpha with ab275745 at 1/1000 (0.542 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on sertoli cells of rat testis. The section was incubated with ab275745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: MOLT-4.(Database:HPA).
Exposure time: Lane 1: 8 seconds Lane2-4: 70 seconds.
All lanes: Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
Lane 3: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 4: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 52 kDa
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Retinoic Acid Receptor alpha with ab275745 at 1/1000 (0.542 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on sertoli cells of mouse testis (PMID:16210368). The section was incubated with ab275745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-RARA antibody [EPR23871-271] (ab275745) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab275745 was shown to bind specifically to RARA. A band was observed at 50-55 kDa in wild-type MCF7 cell lysates with no signal observed at this size in RARA knockout cell line ab277158 (knockout cell lysate ab281383). To generate this image, wild-type and RARA knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (AB275745) at 1/1000 dilution
Lane 1: Wild-type MCF7 cell lysate at 20 µg
Lane 2: RARA knockout MCF7 cell lysate at 20 µg
Lane 3: Human Testis cell lysate at 20 µg
Lane 4: Human Brain cell lysate at 20 µg
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
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