Anti-Retinoic Acid Receptor beta antibody [EPR2017]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (AB124701)

ab124701 at 1/100 dilution staining Retinoic Acid Receptor beta in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (AB124701)

All lanes:

Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) at 1/2000 dilution

Lane 1:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates at 15 µg

Lane 2:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 55 kDa

false

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (AB124701)

Immunocytochemistry/ Immunofluorescence analysis of HCT 116 (Human colorectal carcinoma epithelial cell) cells labeling Retinoic Acid Receptor beta with Purified ab124701 at 1 : 100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (AB124701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Retinoic Acid Receptor beta with Purified ab124701 at 1 : 100 dilution (10.3 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Unknown

Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (AB124701)

All lanes:

Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) at 1/1000 dilution

Lane 1:

MCF7 cell lysates at 10 µg

Lane 2:

MDA-MB-435 cell lysates at 10 µg

Lane 3:

Daudi cell lysates at 10 µg

Lane 4:

U-87 MG cell lysates at 10 µg

Secondary

Lane 1:

Goat anti-Rabbit HRP at 1/20000 dilution

Lanes 2 - 4:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 50 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (AB124701)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.