Rabbit Recombinant Monoclonal Retinoic Acid Receptor beta antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody [EPR2017] - BSA and Azide free (AB247968), expandable thumbnail](https://content.abcam.com/products/images/retinoic-acid-receptor-beta-antibody-epr2017-bsa-and-azide-free-ab247968--immunocytochemistry-immunofluorescence-img71262.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody [EPR2017] - BSA and Azide free (AB247968)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] - BSA and Azide free (AB247968), expandable thumbnail](https://content.abcam.com/products/images/retinoic-acid-receptor-beta-antibody-epr2017-bsa-and-azide-free-ab247968--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113126.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] - BSA and Azide free (AB247968)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Expected | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors (PubMed:12554770). The RXRA/RARB heterodimer can act as a repressor on the DR1 element and as an activator on the DR5 element (PubMed:29021580). In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity).
Alternative names
HAP, NR1B2, RARB, Retinoic acid receptor beta, RAR-beta, HBV-activated protein, Nuclear receptor subfamily 1 group B member 2, RAR-epsilon
Recommended products
Rabbit Recombinant Monoclonal Retinoic Acid Receptor beta antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2017
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.13 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247968 is the carrier-free version of Anti-Retinoic Acid Receptor beta antibody [EPR2017] ab124701.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Retinoic Acid Receptor beta (RARβ) also known as NR1B2 is a nuclear receptor with a molecular mass of approximately 51 kDa. This receptor functions mechanically by binding retinoic acid which induces conformational changes that allow it to regulate gene transcription. RARβ is expressed in various tissues including the central nervous system lungs and kidneys. Its role as a transcription factor influences gene expression patterns by interacting with specific retinoic acid response elements (RARE) in the DNA.
- Biological function summary
Retinoic Acid Receptor beta plays a significant role in cell differentiation and development. It often forms heterodimers with retinoid X receptors (RXRs) to execute its functions. This interaction enhances the receptor's ability to modulate gene expression. The complex orchestrates a broad range of developmental processes particularly in embryogenesis and organogenesis. RARβ influences several cellular activities including the regulation of apoptosis and the maintenance of cellular homeostasis.
- Pathways
The activity of Retinoic Acid Receptor beta integrates predominantly into the retinoic acid signaling pathway and the Wnt signaling pathway. Through these pathways RARβ contributes to the regulation of cell fate and the inhibition of cell proliferation. It functions alongside proteins such as RXRs in the retinoic acid pathway and may interact with components of the Wnt pathway like β-catenin to control gene transcription and cellular responses. These interactions guide various developmental and physiological processes.
- Associated diseases and disorders
Retinoic Acid Receptor beta has connections to cancer and retinal diseases. Changes in RARβ expression and function associate with several types of cancer including lung and breast cancer often involving dysregulation of apoptosis and cell proliferation. In retinal diseases RARβ in conjunction with the protein CRABP (cellular retinoic acid-binding protein) affects the homeostasis and survival of retinal cells. Understanding RARβ's role in these diseases helps in developing targeted therapies aimed at modulating its activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody [EPR2017] - BSA and Azide free (ab247968), expandable thumbnail](https://content.abcam.com/products/images/retinoic-acid-receptor-beta-antibody-epr2017-bsa-and-azide-free-ab247968--immunocytochemistry-immunofluorescence-img71262.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody [EPR2017] - BSA and Azide free (ab247968)")
Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody [EPR2017] - BSA and Azide free (ab247968)
Immunocytochemistry/ Immunofluorescence analysis of HCT 116 (Human colorectal carcinoma epithelial cell) cells labeling Retinoic Acid Receptor beta with Purified Anti-Retinoic Acid Receptor beta antibody [EPR2017] ab124701 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Retinoic Acid Receptor beta antibody [EPR2017] ab124701)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] - BSA and Azide free (ab247968), expandable thumbnail](https://content.abcam.com/products/images/retinoic-acid-receptor-beta-antibody-epr2017-bsa-and-azide-free-ab247968--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113126.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] - BSA and Azide free (ab247968)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] - BSA and Azide free (ab247968)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Retinoic Acid Receptor beta with Purified Anti-Retinoic Acid Receptor beta antibody [EPR2017] ab124701 at 1:100 dilution (10.3 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Retinoic Acid Receptor beta antibody [EPR2017] ab124701)
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