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AB5904

Anti-Retinoic Acid Receptor gamma 1 antibody

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(2 Publications)

Rabbit Polyclonal RARG antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RARG aa 1-100.

View Alternative Names

NR1B3, RARG, Retinoic acid receptor gamma, RAR-gamma, Nuclear receptor subfamily 1 group B member 3

3 Images
Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (AB5904)
  • WB

Supplier Data

Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (AB5904)

Detection : chemiluminescence.

All lanes:

Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) at 1/500 dilution

Lane 1:

MCF7 Nuclear enriched extract lysate at 50 µg

Lane 2:

BeWo Nuclear enriched extract lysate at 50 µg

Lane 3:

HaCaT Nuclear enriched extract lysate at 50 µg

Lane 4:

HepG2 Nuclear enriched extract lysate at 50 µg

Lane 5:

Mouse Mammary Gland tissue lysate at 50 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

Predicted band size: 50 kDa

Observed band size: 60 kDa

false

Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (AB5904)
  • WB

Supplier Data

Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (AB5904)

Detection : chemiluminescence.

All lanes:

Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) at 1/250 dilution

All lanes:

Rat brain tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.25 µg/mL

Predicted band size: 50 kDa

Observed band size: 42 kDa

false

Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (AB5904)
  • WB

Supplier Data

Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (AB5904)

All lanes:

Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)

Lane 1:

HEK293 cell lysate with neutralizing peptide

Lane 2:

HEK293 cell lysate

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human RARG aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P13631

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Retinoic Acid Receptor gamma 1 (RARG) functions as a nuclear receptor and transcription factor which means it binds to specific DNA sequences to regulate gene expression. Also known as RAR gamma this protein has a molecular mass of approximately 51 kDa. RARG is expressed in various tissues most notably in skin lung and the central nervous system. It acts by binding to retinoic acid a derivative of vitamin A which is essential for modulating cell differentiation and growth.
Biological function summary

Retinoic Acid Receptor gamma 1 influences the regulation of several developmental processes and cellular homeostasis. The receptor operates often as part of a heterodimeric complex with the Retinoid X Receptor (RXR). This interaction is important for the receptor's ability to bind to retinoic acid response elements in DNA driving the expression of genes that control cell proliferation apoptosis and development. Its activity impacts skin keratinization and neural differentiation significantly.

Pathways

Retinoic Acid Receptor gamma 1 is key in the retinoic acid signaling pathway which orchestrates the transcription of essential genes linked to cellular differentiation and organogenesis. RARG is closely related to other retinoic acid receptors like RAR alpha and RAR beta in this pathway. These receptors facilitate a network where retinoic acid acts as a ligand initiating changes in gene expression that affect cellular response and fate.

The dysregulation of RARG links to health issues such as skin disorders and certain types of cancer. Psoriasis for example involves abnormal proliferation and differentiation of keratinocytes where RARG may play a role alongside other proteins like RXR. In leukemia alterations in RARG have associations with disrupted differentiation of blood cell lines closely interacting with oncogenic fusion proteins. Understanding RARG's involvement in these conditions helps in developing targeted treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function (By similarity).
See full target information RARG
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 17:74-86 PubMed31255985

2019

Role of Retinoic Acid Receptor-γ in DNA Damage-Induced Necroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chamila Kadigamuwa,Swati Choksi,Qing Xu,Christophe Cataisson,Steven S Greenbaum,Stuart H Yuspa,Zheng-Gang Liu

Pediatric research 73:612-620 PubMed23419538

2013

Intrauterine growth restriction transiently delays alveolar formation and disrupts retinoic acid receptor expression in the lung of female rat pups.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Joss-Moore,Travis Carroll,Yan Yang,Melanie Fitzhugh,Drew Metcalfe,Jake Oman,Merica Hale,Li Dong,Zheng-Ming Wang,Xing Yu,Christopher W Callaway,Elizabeth O'Brien,Robert A McKnight,Robert H Lane,Kurt H Albertine
View all publications
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