Rabbit Polyclonal RARG antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RARG aa 100-450.
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Publications
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- Nature communications 9:14062018Inhibition of cancer stem cell like cells by a synthetic retinoid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunwei Chen et. al.PubMed 29643385
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RARG aa 100-450. The exact immunogen used to generate this antibody is proprietary information. Database link P13631
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Not recommended | Tested |
| Mouse | Not recommended | Predicted |
| Rat | Not recommended | Predicted |
| Cow | Not recommended | Predicted |
| Pig | Not recommended | Predicted |
| Zebrafish | Not recommended | Predicted |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Cow, Pig, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function (By similarity).
Alternative names
Retinoic acid receptor gamma, RAR-gamma, Nuclear receptor subfamily 1 group B member 3, NR1B3, RARG
Recommended products
Rabbit Polyclonal RARG antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RARG aa 100-450.
Alternative names
Retinoic acid receptor gamma, RAR-gamma, Nuclear receptor subfamily 1 group B member 3, NR1B3, RARG
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RARG aa 100-450. The exact immunogen used to generate this antibody is proprietary information. Database link P13631
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Retinoic Acid Receptor gamma (RARγ) also known as NR1B3 is a nuclear receptor that mediates the actions of retinoic acid. This receptor has a molecular mass of approximately 50 kDa. Scientists commonly find RARγ in a variety of tissues including the skin lung and central nervous system. The receptor functions as a transcription factor by binding to specific DNA sequences and regulating the expression of genes involved in cellular proliferation and differentiation.
- Biological function summary
RARγ plays an essential role in modulating gene expression in response to retinoic acid. This receptor forms heterodimeric complexes with retinoid X receptors (RXRs) to initiate transcriptional activation. RARγ part of the nuclear receptor superfamily drives processes such as embryogenesis and cell differentiation by controlling gene networks. Its presence is necessary for proper functioning in many physiological contexts contributing to homeostasis and development.
- Pathways
Retinoic acid signaling involves RARγ and plays a pivotal role in the retinoic acid signaling pathway and the Wnt signaling pathway. RARγ interacts with proteins like RXRs to regulate gene transcription necessary for cell fate decisions and tissue patterning. These pathways link to key cellular processes highlighting the importance of RARγ in transcriptional regulation and cellular development.
- Associated diseases and disorders
RARγ has associations with skin disorders such as psoriasis and certain types of cancer including acute promyelocytic leukemia. Disruptions in retinoic acid signaling implicate this receptor in abnormal cell proliferation and differentiation. In leukemia the receptor partners with RXRs and interacts with fusion proteins like PML-RARα to drive cancerous processes. Understanding RARγ and its interactions contributes to developing treatments targeting these disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Retinoic Acid Receptor gamma antibody (ab97569)
10% SDS PAGE
All lanes: Western blot - Anti-Retinoic Acid Receptor gamma antibody (ab97569) at 1/1000 dilution
All lanes: NIH3T3 whole cell lysate at 30 µg
Predicted band size: 50 kDa
Western blot - Anti-Retinoic Acid Receptor gamma antibody (ab97569)
7.5% SDS PAGE
All lanes: Western blot - Anti-Retinoic Acid Receptor gamma antibody (ab97569) at 1/1000 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody (ab97569)
ab97569, at a 1/200 dilution, staining Retinoic Acid Receptor gamma in paraformaldehyde-fixed HeLa cells, by Immunofluorescence analysis. Right image is merged with a DNA probe.
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