Rabbit Recombinant Monoclonal RARG antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.
Images
![Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159), expandable thumbnail](https://content.abcam.com/products/images/retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-ab187159--western-blot-img144535.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159)")
![Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159), expandable thumbnail](https://content.abcam.com/products/images/retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-ab187159--western-blot-img105937.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159)")
![Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159), expandable thumbnail](https://content.abcam.com/products/images/retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-ab187159--immunocytochemistry-immunofluorescence-img93619.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159)")
Publications
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- Cell stem cell 28:2076-2089.e72021RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTarek Magdy,Zhengxin Jiang,Mariam Jouni,Hananeh Fonoudi,Davi Lyra-Leite,Gwanghyun Jung,Marisol Romero-Tejeda,Hui-Hsuan Kuo,K Ashley Fetterman,Mennat Gharib,Brian T Burmeister,Mingming Zhao,Yadav Sapkota,Colin J Ross,Bruce C Carleton,Daniel Bernstein,Paul W BurridgePubMed 34525346
- European journal of pharmacology 906:1741992021A novel all-trans retinoic acid derivative regulates cell cycle and differentiation of myelodysplastic syndrome cells by USO1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShufang Li,Ge Deng,Jingwen Su,Ke Wang,Cong Wang,Lanlan Li,Sujing Song,Xiaoqing Peng,Feihu ChenPubMed 34058203
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Target data
Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function (By similarity).
Alternative names
NR1B3, RARG, Retinoic acid receptor gamma, RAR-gamma, Nuclear receptor subfamily 1 group B member 3
Recommended products
Rabbit Recombinant Monoclonal RARG antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR2020(N)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Retinoic Acid Receptor gamma (RARγ) also known as NR1B3 is a nuclear receptor that mediates the actions of retinoic acid. This receptor has a molecular mass of approximately 50 kDa. Scientists commonly find RARγ in a variety of tissues including the skin lung and central nervous system. The receptor functions as a transcription factor by binding to specific DNA sequences and regulating the expression of genes involved in cellular proliferation and differentiation.
- Biological function summary
RARγ plays an essential role in modulating gene expression in response to retinoic acid. This receptor forms heterodimeric complexes with retinoid X receptors (RXRs) to initiate transcriptional activation. RARγ part of the nuclear receptor superfamily drives processes such as embryogenesis and cell differentiation by controlling gene networks. Its presence is necessary for proper functioning in many physiological contexts contributing to homeostasis and development.
- Pathways
Retinoic acid signaling involves RARγ and plays a pivotal role in the retinoic acid signaling pathway and the Wnt signaling pathway. RARγ interacts with proteins like RXRs to regulate gene transcription necessary for cell fate decisions and tissue patterning. These pathways link to key cellular processes highlighting the importance of RARγ in transcriptional regulation and cellular development.
- Associated diseases and disorders
RARγ has associations with skin disorders such as psoriasis and certain types of cancer including acute promyelocytic leukemia. Disruptions in retinoic acid signaling implicate this receptor in abnormal cell proliferation and differentiation. In leukemia the receptor partners with RXRs and interacts with fusion proteins like PML-RARα to drive cancerous processes. Understanding RARγ and its interactions contributes to developing treatments targeting these disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159), expandable thumbnail](https://content.abcam.com/products/images/retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-ab187159--western-blot-img144535.jpg "Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)")
Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
Lanes 1 - 3: Merged signal (red and green). Green - ab187159 observed at 50 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.ab187159 was shown to recognize Acid Receptor gamma in wild-type HAP1 cells as signal was lost at the expected MW in RARG (Acid Receptor gamma) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RARG (Acid Receptor gamma) knockout samples were subjected to SDS-PAGE. ab187159 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 30 µg
Lane 2: RARG (Acid Receptor gamma) knockout HAP1 whole cell lysate at 30 µg
Lane 3: A375 whole cell lysate at 30 µg
Predicted band size: 50 kDa
Observed band size: 50 kDa
![Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159), expandable thumbnail](https://content.abcam.com/products/images/retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-ab187159--western-blot-img105937.jpg "Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)")
Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
All lanes: Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution
Lane 1: NCCIT cell lysate at 20 µg
Lane 2: NTERA2/D1 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159), expandable thumbnail](https://content.abcam.com/products/images/retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-ab187159--immunocytochemistry-immunofluorescence-img93619.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)")
Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
Immunofluorescent analysis of HeLa cells labeling Retinoic Acid Receptor gamma with ab187159 at 1/250 and DAPI staining (blue).
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