AB187159

Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal

RabMAb
Recombinant
KO Validated
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(4 Publications)

Rabbit Recombinant Monoclonal RARG antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

NR1B3, RARG, Retinoic acid receptor gamma, RAR-gamma, Nuclear receptor subfamily 1 group B member 3

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159)

Immunofluorescent analysis of HeLa cells labeling Retinoic Acid Receptor gamma with ab187159 at 1/250 and DAPI staining (blue).

Lab

Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159)

Lanes 1 - 3 : Merged signal (red and green). Green - ab187159 observed at 50 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab187159 was shown to recognize Acid Receptor gamma in wild-type HAP1 cells as signal was lost at the expected MW in RARG (Acid Receptor gamma) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RARG (Acid Receptor gamma) knockout samples were subjected to SDS-PAGE. ab187159 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 30 µg

Lane 2:

RARG (Acid Receptor gamma) knockout HAP1 whole cell lysate at 30 µg

Lane 3:

A375 whole cell lysate at 30 µg

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Supplier Data

Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159)

All lanes:

Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution

Lane 1:

NCCIT cell lysate at 20 µg

Lane 2:

NTERA2/D1 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

false

Carrier free

Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2020(N)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Retinoic Acid Receptor gamma (RARγ) also known as NR1B3 is a nuclear receptor that mediates the actions of retinoic acid. This receptor has a molecular mass of approximately 50 kDa. Scientists commonly find RARγ in a variety of tissues including the skin lung and central nervous system. The receptor functions as a transcription factor by binding to specific DNA sequences and regulating the expression of genes involved in cellular proliferation and differentiation.

Biological function summary

RARγ plays an essential role in modulating gene expression in response to retinoic acid. This receptor forms heterodimeric complexes with retinoid X receptors (RXRs) to initiate transcriptional activation. RARγ part of the nuclear receptor superfamily drives processes such as embryogenesis and cell differentiation by controlling gene networks. Its presence is necessary for proper functioning in many physiological contexts contributing to homeostasis and development.

Pathways

Retinoic acid signaling involves RARγ and plays a pivotal role in the retinoic acid signaling pathway and the Wnt signaling pathway. RARγ interacts with proteins like RXRs to regulate gene transcription necessary for cell fate decisions and tissue patterning. These pathways link to key cellular processes highlighting the importance of RARγ in transcriptional regulation and cellular development.

RARγ has associations with skin disorders such as psoriasis and certain types of cancer including acute promyelocytic leukemia. Disruptions in retinoic acid signaling implicate this receptor in abnormal cell proliferation and differentiation. In leukemia the receptor partners with RXRs and interacts with fusion proteins like PML-RARα to drive cancerous processes. Understanding RARγ and its interactions contributes to developing treatments targeting these disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function (By similarity).

See full target information RARG

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:617 PubMed39805831

2025

Oncogenic role of RARG rearrangements in acute myeloid leukemia resembling acute promyelocytic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wang,Luyao Zhao,Yun Tan,Xufeng Cen,Huan Gao,Huimin Jiang,Ying Liu,Yunxuan Li,Tingting Zhang,Chenxi Zhao,Ting Shi,Guilin Xu,Churan Wang,Jiong Hu,Xia Li,Ya-Zhen Qin,Kankan Wang,Hong-Hu Zhu,Ke Li

Cell stem cell 28:2076-2089.e7 PubMed34525346

2021

RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Tarek Magdy,Zhengxin Jiang,Mariam Jouni,Hananeh Fonoudi,Davi Lyra-Leite,Gwanghyun Jung,Marisol Romero-Tejeda,Hui-Hsuan Kuo,K Ashley Fetterman,Mennat Gharib,Brian T Burmeister,Mingming Zhao,Yadav Sapkota,Colin J Ross,Bruce C Carleton,Daniel Bernstein,Paul W Burridge

European journal of pharmacology 906:174199 PubMed34058203

2021

A novel all-trans retinoic acid derivative regulates cell cycle and differentiation of myelodysplastic syndrome cells by USO1.

Applications

Unspecified application

Species

Unspecified reactive species

Shufang Li,Ge Deng,Jingwen Su,Ke Wang,Cong Wang,Lanlan Li,Sujing Song,Xiaoqing Peng,Feihu Chen

Open life sciences 13:404-412 PubMed33817109

2018

Long Non-coding RNA-2271 Promotes Osteogenic Differentiation in Human Bone Marrow Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Cheng Xi,Hong-Yu Li,Dong Yin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159)

Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159)

Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise