Mouse Monoclonal Retinoid X Receptor beta/RXRB antibody. Carrier free. Suitable for ICC, Flow Cyt, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RXRB.
Images
![Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (AB277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--immunocytochemistry-img21588.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (AB277119)")
![Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (AB277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--flow-cytometry-img59455.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (AB277119)")
![Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (AB277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--flow-cytometry-img55061.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (AB277119)")
![Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (AB277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--immunocytochemistry-img103569.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (AB277119)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (AB277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80770.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (AB277119)")
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human RXRB. Database link P28702
Reactivity data
Select an application| ICC | Flow Cyt | Protein Array | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE).
Alternative names
Retinoic acid receptor RXR-beta, Nuclear receptor subfamily 2 group B member 2, Retinoid X receptor beta, RXRB, NR2B2
Recommended products
Mouse Monoclonal Retinoid X Receptor beta/RXRB antibody. Carrier free. Suitable for ICC, Flow Cyt, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RXRB.
Alternative names
Retinoic acid receptor RXR-beta, Nuclear receptor subfamily 2 group B member 2, Retinoid X receptor beta, RXRB, NR2B2
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human RXRB. Database link P28702
- Carrier free
Yes
- Clone number
PCRP-RXRB-2B6
- Purification technique
Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from bioreactor concentrate
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
Retinoid X Receptor Beta (RXRB) also known as NR2B2 is a nuclear receptor with a molecular mass of approximately 54 kDa. This receptor is widely expressed in various tissues including liver kidney and muscle. RXRB functions as a ligand-activated transcription factor meaning it regulates gene expression in response to binding with specific ligands. It partners with other nuclear receptors to form heterodimers allowing it to bind with high affinity to response elements in DNA regulating a wide variety of genes important for metabolic processes.
- Biological function summary
RXRB contributes significantly to the regulation of gene networks by forming heterodimers with other nuclear receptors like PPARs (Peroxisome Proliferator-Activated Receptors) and LXR (Liver X Receptor). These complexes enable RXRB to exert control over diverse biological functions such as lipid metabolism glucose homeostasis and detoxification processes. RXRB influences these processes by modulating the expression of genes involved in the synthesis transport and metabolism of lipids and glucose important for maintaining metabolic homeostasis.
- Pathways
RXRB plays an integral role in the retinoic acid signaling pathway and the nuclear receptor signaling pathway. In the retinoic acid pathway RXRB partners with Retinoic Acid Receptors (RARs) to regulate transcription of target genes important for cell differentiation and proliferation. Within the nuclear receptor signaling pathway RXRB frequently interacts with proteins such as PPARs and LXRs impacting lipid metabolism by influencing adipogenesis and cholesterol transport. These interactions underline RXRB's central position in managing metabolic pathways.
- Associated diseases and disorders
RXRB is linked to metabolic disorders including type 2 diabetes and dyslipidemia. Dysregulation of RXRB can lead to impaired lipid metabolism and insulin resistance impacting blood sugar and lipid profiles. The receptor interacts with PPARs in these conditions as they share regulatory roles in lipid and glucose metabolism. Additionally alterations in RXRB activity are linked to certain cancers with the receptor being part of pathways that regulate cell growth and differentiation. RXRB's role in these processes highlights its potential as a therapeutic target for managing metabolic diseases and cancer.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--immunocytochemistry-img21588.jpg "Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)")
Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
This data was developed using Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Retinoid X Receptor beta/RXRB with Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104 at 2 μg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).
![Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--flow-cytometry-img59455.jpg "Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)")
Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
This data was developed using Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104, the same antibody clone in a different buffer formulation.
Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Retinoid X Receptor beta/RXRB using Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104 at 2 μg/106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
![Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--flow-cytometry-img55061.jpg "Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)")
Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
This data was developed using Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104, the same antibody clone in a different buffer formulation.
Flow cytometry analysis of PFA-fixed U87 (human glioblastoma-astrocytoma epithelial cell line) cells labeling Retinoid X Receptor beta/RXRB using Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104 at 2 μg/106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
![Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--immunocytochemistry-img103569.jpg "Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)")
Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
This data was developed using Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Retinoid X Receptor beta/RXRB with Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104 at 2 μg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80770.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
This data was developed using Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104, the same antibody clone in a different buffer formulation.
Formalin-fixed, paraffin-embedded human lymph node tissue stained for Retinoid X Receptor beta/RXRB using Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104 at 2 μg/ml in immunohistochemical analysis.
![Protein Array - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--protein-array-img119754.jpg "Protein Array - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)")
Protein Array - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
This data was developed using Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104, the same antibody clone in a different buffer formulation.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] ab277104.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
![Protein Array - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119), expandable thumbnail](https://content.abcam.com/products/images/retinoid-x-receptor-beta-rxrb-antibody-pcrp-rxrb-2b6-bsa-and-azide-free-ab277119--protein-array-img119754.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)")
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