Rabbit Polyclonal Retinol dehydrogenase 16 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RDH16 aa 150-300.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RDH16 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link O75452
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Target data
Function
Oxidoreductase with a preference for NAD. Oxidizes all-trans-retinol, 9-cis-retinol, 11-cis-retinol and 13-cis-retinol to the corresponding aldehydes (PubMed:10329026, PubMed:12534290, PubMed:9677409). Has higher activity towards CRBP-bound retinol than with free retinol (PubMed:12534290). Oxidizes also 3-alpha-hydroxysteroids. Oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. Can also catalyze the reverse reaction (PubMed:10329026, PubMed:9677409, PubMed:29541409).
Alternative names
Retinol dehydrogenase 16, Human epidermal retinol dehydrogenase, Microsomal NAD(+)-dependent retinol dehydrogenase 4, Short chain dehydrogenase/reductase family 9C member 8, Sterol/retinol dehydrogenase, hRDH-E, RoDH-4, RODH4, RDH16, SDR9C8
Recommended products
Rabbit Polyclonal Retinol dehydrogenase 16 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RDH16 aa 150-300.
Alternative names
Retinol dehydrogenase 16, Human epidermal retinol dehydrogenase, Microsomal NAD(+)-dependent retinol dehydrogenase 4, Short chain dehydrogenase/reductase family 9C member 8, Sterol/retinol dehydrogenase, hRDH-E, RoDH-4, RODH4, RDH16, SDR9C8
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RDH16 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link O75452
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Retinol dehydrogenase 16 (RDH16) also known as PED1 plays an important role in the oxidation of retinol to retinal. This enzyme has a mass of approximately 35 kDa. RDH16 is expressed mainly in the liver as well as in the kidney and intestine where it facilitates the metabolism of retinoids essential compounds for various biological processes. RDH16 acts by converting all-trans-retinol to all-trans-retinal a critical step in retinoid metabolism.
- Biological function summary
RDH16 contributes significantly to the biosynthesis of retinoic acid a derivative of vitamin An important for cell differentiation and proliferation. While RDH16 does not form part of a larger known complex its activity directly influences the levels of retinoic acid affecting signaling pathways involved in embryonic development vision and immune function. These interactions highlight RDH16's role in modulating important cellular functions through retinoid metabolism.
- Pathways
The enzymatic activity of RDH16 integrates into the retinoic acid signaling and metabolic pathways. This activity involves interconversions in the retinoid cycle that are necessary for the availability of retinoic acid in tissues. RDH16 also functions alongside other enzymes such as retinol dehydrogenase 10 (RDH10) and retinaldehyde dehydrogenase enzymes ensuring a balance in the production of this signaling molecule which can influence cell signaling and gene expression.
- Associated diseases and disorders
Abnormal function or expression of RDH16 has ties to metabolic diseases such as obesity and non-alcoholic fatty liver disease (NAFLD). These conditions are linked to disrupted retinoid metabolism highlighting the importance of RDH16 in maintaining metabolic health. Additionally RDH16's involvement with the production of retinoic acid connects it to conditions associated with vitamin A deficiency where interactions with proteins such as retinoic acid receptors (RARs) are noticeably impacted further emphasizing its role in disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (ab224163)
Immunohistochemistry analysis of human liver tissue labeling RDH16 with ab224163 at 1:200.
Western blot - Anti-Retinol dehydrogenase 16 antibody (ab224163)
Western blot analysis of human liver tissue lysate with ab224163 at 0.4 μg/ml.
All lanes: Western blot - Anti-Retinol dehydrogenase 16 antibody (ab224163) at 0.4 µg/mL
Predicted band size: 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (ab224163)
Immunohistochemistry analysis of human pancreatic tissue labeling RDH16 with ab224163 at 1:200. Negative control showing lack of staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (ab224163)
Immunohistochemistry analysis of human kidney tissue labeling RDH16 with ab224163 at 1:200. Negative control showing lack of staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (ab224163)
Immunohistochemistry analysis of human lymph node tissue labeling RDH16 with ab224163 at 1:200. Negative control showing lack of staining.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com