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AB224163

Anti-Retinol dehydrogenase 16 antibody

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(2 Publications)

Rabbit Polyclonal Retinol dehydrogenase 16 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RDH16 aa 150-300.

View Alternative Names

RODH4, SDR9C8, RDH16, Retinol dehydrogenase 16, Human epidermal retinol dehydrogenase, Microsomal NAD(+)-dependent retinol dehydrogenase 4, Short chain dehydrogenase/reductase family 9C member 8, Sterol/retinol dehydrogenase, hRDH-E, RoDH-4

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (AB224163)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (AB224163)

Immunohistochemistry analysis of human liver tissue labeling RDH16 with ab224163 at 1 : 200.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (AB224163)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (AB224163)

Immunohistochemistry analysis of human pancreatic tissue labeling RDH16 with ab224163 at 1 : 200. Negative control showing lack of staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (AB224163)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (AB224163)

Immunohistochemistry analysis of human kidney tissue labeling RDH16 with ab224163 at 1 : 200. Negative control showing lack of staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (AB224163)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinol dehydrogenase 16 antibody (AB224163)

Immunohistochemistry analysis of human lymph node tissue labeling RDH16 with ab224163 at 1 : 200. Negative control showing lack of staining.

Western blot - Anti-Retinol dehydrogenase 16 antibody (AB224163)
  • WB

Supplier Data

Western blot - Anti-Retinol dehydrogenase 16 antibody (AB224163)

Western blot analysis of human liver tissue lysate with ab224163 at 0.4 μg/ml.

All lanes:

Western blot - Anti-Retinol dehydrogenase 16 antibody (ab224163) at 0.4 µg/mL

Predicted band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RDH16 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

O75452

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Retinol dehydrogenase 16 (RDH16) also known as PED1 plays an important role in the oxidation of retinol to retinal. This enzyme has a mass of approximately 35 kDa. RDH16 is expressed mainly in the liver as well as in the kidney and intestine where it facilitates the metabolism of retinoids essential compounds for various biological processes. RDH16 acts by converting all-trans-retinol to all-trans-retinal a critical step in retinoid metabolism.
Biological function summary

RDH16 contributes significantly to the biosynthesis of retinoic acid a derivative of vitamin An important for cell differentiation and proliferation. While RDH16 does not form part of a larger known complex its activity directly influences the levels of retinoic acid affecting signaling pathways involved in embryonic development vision and immune function. These interactions highlight RDH16's role in modulating important cellular functions through retinoid metabolism.

Pathways

The enzymatic activity of RDH16 integrates into the retinoic acid signaling and metabolic pathways. This activity involves interconversions in the retinoid cycle that are necessary for the availability of retinoic acid in tissues. RDH16 also functions alongside other enzymes such as retinol dehydrogenase 10 (RDH10) and retinaldehyde dehydrogenase enzymes ensuring a balance in the production of this signaling molecule which can influence cell signaling and gene expression.

Abnormal function or expression of RDH16 has ties to metabolic diseases such as obesity and non-alcoholic fatty liver disease (NAFLD). These conditions are linked to disrupted retinoid metabolism highlighting the importance of RDH16 in maintaining metabolic health. Additionally RDH16's involvement with the production of retinoic acid connects it to conditions associated with vitamin A deficiency where interactions with proteins such as retinoic acid receptors (RARs) are noticeably impacted further emphasizing its role in disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Oxidoreductase with a preference for NAD. Oxidizes all-trans-retinol, 9-cis-retinol, 11-cis-retinol and 13-cis-retinol to the corresponding aldehydes (PubMed : 10329026, PubMed : 12534290, PubMed : 9677409). Has higher activity towards CRBP-bound retinol than with free retinol (PubMed : 12534290). Oxidizes also 3-alpha-hydroxysteroids. Oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. Can also catalyze the reverse reaction (PubMed : 10329026, PubMed : 29541409, PubMed : 9677409).
See full target information RDH16
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:513 PubMed40210831

2025

Relationship between retinol metabolism and hepatocellular carcinoma: a comprehensive analysis of Mendelian randomization, prognostic characteristic and experiment.

Applications

Unspecified application

Species

Unspecified reactive species

Fuqing Chen,Yifan Cai,Jianyin Zhou

Nature 637:496-503 PubMed39633052

2024

Mis-splicing of a neuronal microexon promotes CPEB4 aggregation in ASD.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Garcia-Cabau,Anna Bartomeu,Giulio Tesei,Kai Chit Cheung,Julia Pose-Utrilla,Sara Picó,Andreea Balaceanu,Berta Duran-Arqué,Marcos Fernández-Alfara,Judit Martín,Cesare De Pace,Lorena Ruiz-Pérez,Jesús García,Giuseppe Battaglia,José J Lucas,Rubén Hervás,Kresten Lindorff-Larsen,Raúl Méndez,Xavier Salvatella
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Product promise

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