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AB75328

Anti-REV1 antibody

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Rabbit Polyclonal REV1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human REV1.

View Alternative Names

REV1L, REV1, DNA repair protein REV1, Alpha integrin-binding protein 80, Rev1-like terminal deoxycytidyl transferase, AIBP80

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human REV1. The exact immunogen used to generate this antibody is proprietary information.

Q9UBZ9

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Reactivity data

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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REV1 also known as REV1 DNA-directed polymerase functions as a DNA polymerase specialized in translesion synthesis. Weighing approximately 125 kDa REV1 facilitates bypass of DNA lesions during replication ensuring continuous DNA synthesis. This protein acts primarily as a deoxycytidyl transferase inserting dCMP opposite damaged nucleotides. REV1 is expressed in various tissues but shows higher expression levels in testis and thymus. It is pivotal in maintaining genome stability under genotoxic stress.
Biological function summary

REV1 operates within the context of DNA repair and a larger polymerase zeta complex working closely with other translesion DNA polymerases. REV1 interacts with other translesion synthesis proteins like POLH POLL and POLK to orchestrate the bypass of different types of DNA damage. These interactions enable REV1 to play a role in cellular responses to DNA damage and contribute to maintaining genome integrity.

Pathways

REV1 integrates into the DNA damage response and translesion synthesis pathways. In these pathways REV1 coordinates with REV3L and REV7 forming part of the polymerase zeta complex to process lesions bypass that regular DNA polymerases cannot handle. Significantly it associates with the Fanconi anemia pathway affecting DNA repair mechanisms under stress. These collaborations help manage replication stress and stabilize cellular division.

REV1 is associated with cancer and immunodeficiency conditions. Abnormalities in its function correlate with faulty translesion synthesis leading to genomic instability implicated in cancer development. Furthermore in conjunction with BRCA1 and BRCA2 issues with REV1 may impact DNA repair fidelity linking it to breast cancer susceptibility. Understanding the role of REV1 in these disorders could lead to therapeutic interventions targeting its pathway interactions and activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
See full target information REV1
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