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AB111729

Anti-REV3L antibody

5

(1 Review)

|

(2 Publications)

Rabbit Polyclonal REV3L antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human REV3L aa 200-300.

View Alternative Names

POLZ, REV3, REV3L, DNA polymerase zeta catalytic subunit, Protein reversionless 3-like, REV3-like, hREV3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REV3L antibody (AB111729)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REV3L antibody (AB111729)

ab111729 at 1/50 dilution staining REV3L in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human REV3L aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

O60673

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab111729 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REV3L also known as DNA polymerase zeta catalytic subunit or hREV3 is an essential component of the DNA polymerase zeta (Pol ζ) complex. This protein has an approximate molecular weight of 353 kDa. It carries out its functions in several tissues with main expression in the testis thymus and ovaries although it appears in other tissues as well. Mechanically REV3L serves as the catalytic component in DNA synthesis particularly facilitating translesion DNA synthesis which allows DNA replication to bypass lesions or damage.
Biological function summary

DNA damage tolerance mechanisms involve REV3L where it plays a critical role as part of the Pol ζ complex. Pol ζ operates by extending DNA synthesis across non-instructive damages that disrupt the DNA double helix. This action involves error-prone processes but remains necessary for maintaining genomic integrity. REV3L binds with the REV7 protein in this complex to perform these translesion DNA synthesis actions shielding cells from potentially lethal damage or genomic instability due to unrepairable DNA lesions.

Pathways

The functionality of REV3L integrates into the DNA damage response and repair pathway alongside general DNA replication mechanisms. In the DNA damage response REV3L works closely with the RAD6/RAD18 pathway providing an alternate pathway for lesion bypass. The connection to other proteins like REV1 and the involvement in the broader DNA replication and repair processes highlight its important roles in cellular responses to mutagenic lesions as well as normal DNA replication processes.

Research links REV3L to cancer development particularly for tumors with high mutational rates or those exposed to genotoxic stress. The deficiency of REV3L increases susceptibility to DNA damage-induced apoptosis or uncontrolled cellular proliferation. Through its pathways REV3L also interlinks with the BRCA1 protein known for its ties to breast and ovarian cancers. The involvement of REV3L in these critical processes positions it as an important factor in understanding and potentially targeting therapy for cancers driven by DNA damage and repair pathway dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit of the DNA polymerase zeta complex, an error-prone polymerase specialized in translesion DNA synthesis (TLS). Lacks an intrinsic 3'-5' exonuclease activity and thus has no proofreading function.
See full target information REV3L
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 11:676 PubMed32826865

2020

LncRNA CRNDE facilitates epigenetic suppression of CELF2 and LATS2 to promote proliferation, migration and chemoresistance in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Cai Xie,Jian-Quan Zhang,Xi-Li Jiang,Yong-Yong Hua,Shao-Wei Xie,Ye-Ang Qin,Yi-Jun Yang

Open life sciences 15:488-500 PubMed33817237

2020

Lnc-PICSAR contributes to cisplatin resistance by miR-485-5p/REV3L axis in cutaneous squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Wang,Xiaoqiang Zhou,Jing Yin,Yang Zhou
View all publications
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Product promise

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