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AB175431

Anti-Rex1 antibody [5E11E7]

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(3 Publications)

Mouse Monoclonal Rex1 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Transfected cell lysate - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZFP42 aa 200 to C-terminus.

View Alternative Names

REX1, ZNF754, ZFP42, Zinc finger protein 42 homolog, Zfp-42, Reduced expression protein 1, Zinc finger protein 754, REX-1, hREX-1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rex1 antibody [5E11E7] (AB175431)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rex1 antibody [5E11E7] (AB175431)

Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling Rex1 with ab175431 at 1/200 dilution with DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rex1 antibody [5E11E7] (AB175431)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rex1 antibody [5E11E7] (AB175431)

Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling Rex1 with ab175431 at 1/200 dilution with DAB staining.

Flow Cytometry - Anti-Rex1 antibody [5E11E7] (AB175431)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Rex1 antibody [5E11E7] (AB175431)

Flow cytometric analysis of HEK293 cells labeling Rex1 with ab175431 at 1/200 dilution (green) compared to a negative control (red).

Western blot - Anti-Rex1 antibody [5E11E7] (AB175431)
  • WB

Supplier Data

Western blot - Anti-Rex1 antibody [5E11E7] (AB175431)

All lanes:

Western blot - Anti-Rex1 antibody [5E11E7] (ab175431) at 1/500 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

HEK293 cell lysate

Lane 3:

Raji cell lysate

Lane 4:

PC-3 cell lysate

Predicted band size: 35 kDa

false

Western blot - Anti-Rex1 antibody [5E11E7] (AB175431)
  • WB

Supplier Data

Western blot - Anti-Rex1 antibody [5E11E7] (AB175431)

Expected MWt is 32.7 kDa.

All lanes:

Western blot - Anti-Rex1 antibody [5E11E7] (ab175431) at 1/500 dilution

All lanes:

Rex1 recombinant protein

Predicted band size: 35 kDa

false

Western blot - Anti-Rex1 antibody [5E11E7] (AB175431)
  • WB

Supplier Data

Western blot - Anti-Rex1 antibody [5E11E7] (AB175431)

All lanes:

Western blot - Anti-Rex1 antibody [5E11E7] (ab175431) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Rex1 (aa 249-310) - hIgGFc transfected HEK293 cell lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5E11E7

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human ZFP42 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96MM3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rex1 also known as Zfp42 is a transcription factor and zinc finger protein with a mass of approximately 42 kDa. This protein is expressed mostly in embryonic stem cells and some adult stem cells. Rex1 functions by binding to DNA sequences influencing gene expression that is essential for maintaining stem cell self-renewal and pluripotency. As a result Rex1 plays an important role in regulating cellular processes related to developmental potential.
Biological function summary

Rex1 influences stem cell pluripotency and differentiation. It is part of the network of pluripotency factors along with Oct4 Sox2 and Nanog. These proteins form a regulatory circuit that maintains embryonic stem cell identity. Rex1 directly interacts with chromatin structure facilitating the accessibility of specific genes related to stem cell maintenance and identity. The expression levels of Rex1 have a significant impact on the transition between pluripotency and differentiated states within cells.

Pathways

Rex1 is involved in key signaling pathways regulating pluripotency like the LIF/STAT3 and Wnt signaling pathways. Within these pathways Rex1 collaborates with other transcription factors to promote the expression of genes required for stem cell growth and maintenance. Rex1 and Nanog work closely in these pathways to sustain the pluripotent status of stem cells by activating necessary genetic programs and preventing differentiation signals.

Rex1 has connections to certain cancers such as teratocarcinomas due to its role in maintaining pluripotency. Another protein Oct4 often has elevated levels alongside Rex1 in these cancerous cells which drives the tumor's stem-like characteristics. Rex1 levels have been associated with tumor formation and progression in some cancer types suggesting that it could be a biomarker for cancer stem cells and a potential target for therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter. Involved in ES cell self-renewal (By similarity).
See full target information ZFP42
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 49:1197-1208 PubMed40616572

2025

COMMD1 Inhibits Epithelial Mesenchymal Transition (EMT) and Liver Metastasis in Cervical Cancer Through Modulation of the Twist1/E-Cadherin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Xiuli Wang,Ke Wang,Lei Sun,Shiying Yang,Xiaoguang Wang

Cardiovascular drugs and therapy 38:861-872 PubMed36892683

2023

Pharmacological Inhibition of P-Rex1/Rac1 Axis Blocked Angiotensin II-Induced Cardiac Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyuan Pan,Ming Liu,Huimin Su,Hao Hu,Hongwu Chen,Likun Ma

Human cell 36:602-611 PubMed36586053

2023

Expansion of human amniotic epithelial cells using condition cell reprogramming technology.

Applications

Unspecified application

Species

Unspecified reactive species

Aisha Naeem,Muhammad Umer Choudhry,Alex Kroemer,Simone Wahnschafft,Wanxing Cui,Chris Albanese
View all publications
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