Anti-Rex1 antibody [RP23040041]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rex1 antibody [RP23040041] (AB308003)

Immunofluorescent analysis of Rex1 in HeLa cells using ab308003 at 1-3 µg/mL dilution followed by detection using an Alexa Fluor® 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B). Actin stained with Alexa Fluor® 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of Rex1.

• WB

Supplier Data

Western blot - Anti-Rex1 antibody [RP23040041] (AB308003)

Western blot analysis of Rex1 in whole cell extracts of A431 (lane 1) and rat kidney lysate (lane 2) using ab308003 at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~35kDa.

All lanes:

Western blot - Anti-Rex1 antibody [RP23040041] (ab308003) at 1 µg/mL

Lane 1:

A431 whole cell lysate

Lane 2:

rat kidney lysate

Observed band size: 35 kDa

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• WB

Supplier Data

Western blot - Anti-Rex1 antibody [RP23040041] (AB308003)

Western blot analysis of Rex1 was performed by loading 20 µg of HeLa, 3T3, NRK, MDCK, and Cos7 whole cell lysates and 5uL of prestained protein ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% milk in TBST for 1 hour at room temperature. Rex1 was detected at ~35 kD using ab308003 at a concentration of 2 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by a Goat anti-Rabbit IgG-HRP secondary antibody at a concentration of 1 µg/mL for at least 30 minutes at room temperature. Chemiluminescent detection was performed.

All lanes:

Western blot - Anti-Rex1 antibody [RP23040041] (ab308003) at 2 µg/mL

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

3T3 whole cell lysate at 20 µg

Lane 3:

NRK whole cell lysate at 20 µg

Lane 4:

MDCK whole cell lysate at 20 µg

Lane 5:

Cos7 whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG secondary antibody at 1 µg/mL

Observed band size: 35 kDa

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