AB206694

Anti-REXO2 antibody

Name: Anti-REXO2 antibody (ab206694) | Abcam
Brand: Abcam Limited
SKU: AB206694

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(4 Publications)

Rabbit Polyclonal REXO2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human REXO2 aa 150 to C-terminus.

View Alternative Names

SFN, SMFN, CGI-114, REXO2, RNA exonuclease 2 homolog, Small fragment nuclease

2 Images

Supplier Data

Immunoprecipitation - Anti-REXO2 antibody (AB206694)

Detection of REXO2 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab206694 at 6 μg/mg lysate for IP (Lane

and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-REXO2 antibody (ab206694)

Predicted band size: 27 kDa

false

Supplier Data

Western blot - Anti-REXO2 antibody (AB206694)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-REXO2 antibody (ab206694) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 27 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human REXO2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3B8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab206694 was affinity purified using an epitope specific to REXO2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REXO2 also referred to as RNase R or Oligoribonuclease is an enzyme with a molecular mass of around 26 kDa. It plays a significant role in the final steps of RNA decay. Mechanically REXO2 functions as an oligoribonuclease catalyzing the degradation of small ribonucleic acids into mononucleotides. This protein is primarily expressed in the mitochondria of the cell where it ensures the proper completion of RNA turnover.

Biological function summary

The enzyme facilitates the maintenance of cellular RNA quality. REXO2 is not a part of any larger complex but its exonuclease activity allows cells to eliminate short RNA fragments which could otherwise accumulate and lead to cellular dysfunction. Efficient removal of these fragments is essential for normal mitochondrial function and energy production within the cell.

Pathways

This enzyme is key to ensuring RNA metabolic balance. It plays a role in the RNA degradation pathway helping to process and clear unnecessary oligoribonucleotides. While REXO2 does not directly interact with other proteins in a complex it works alongside proteins such as PNPase and RNAse II which facilitate different stages of RNA degradation and processing within the cell.

REXO2 has implications in mitochondrial pathologies. Deficiencies or mutations in this enzyme can contribute to mitochondrial disorders which involve proteins such as POLG and Twinkle. Additionally its efficient RNA clearing function ties it to studies on neurodegenerative diseases where aberrant RNA processing can occur. Understanding these connections allows for deeper insight into how deviations in RNA management can translate into disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

3'-to-5'exoribonuclease that preferentially degrades DNA and RNA oligonucleotides composed of only two nucleotides (PubMed : 23741365, PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Binds and degrades longer oligonucleotides with a lower affinity (PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Plays dual roles in mitochondria, scavenging nanoRNAs (small RNA oligonucleotides of <5 nucleotides) that are produced by the degradosome and clearing short RNAs that are generated by RNA processing (PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Essential for correct initiation of mitochondrial transcription, degrading mitochondrial RNA dinucleotides to prevent RNA-primed transcription at non-canonical sites in the mitochondrial genome (PubMed : 31588022). Essential for embryonic development (By similarity).. Isoform 3. 3'-to-5'exoribonuclease that preferentially degrades DNA and RNA oligonucleotides composed of only two nucleotides.

See full target information REXO2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:6685 PubMed39107301

2024

Heterozygous de novo dominant negative mutation of REXO2 results in interferonopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Elina Idiiatullina,Mahmoud Al-Azab,Meng Lin,Katja Hrovat-Schaale,Ziyang Liu,Xiaotian Li,Caiqin Guo,Xixi Chen,Yaoying Li,Song Gao,Jun Cui,Wenhao Zhou,Li Liu,Yuxia Zhang,Seth L Masters

Discover. Oncology 13:50 PubMed35731336

2022

Circ_0000705 facilitates proline metabolism of esophageal squamous cell carcinoma cells by targeting miR-621/PYCR1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cui-Juan Qian,Yi-Yang Tong,Lin-Ken Wu,Yi-Chao Wang,Xiao-Sheng Teng,Jun Yao

Nucleic acids research 48:5572-5590 PubMed32365187

2020

Human REXO2 controls short mitochondrial RNAs generated by mtRNA processing and decay machinery to prevent accumulation of double-stranded RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Maciej Szewczyk,Deepshikha Malik,Lukasz S Borowski,Sylwia D Czarnomska,Anna V Kotrys,Kamila Klosowska-Kosicka,Marcin Nowotny,Roman J Szczesny

Molecular cell 76:784-796.e6 PubMed31588022

2019

Dinucleotide Degradation by REXO2 Maintains Promoter Specificity in Mammalian Mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Nicholls,Henrik Spåhr,Shan Jiang,Stefan J Siira,Camilla Koolmeister,Sushma Sharma,Johanna H K Kauppila,Min Jiang,Volkhard Kaever,Oliver Rackham,Andrei Chabes,Maria Falkenberg,Aleksandra Filipovska,Nils-Göran Larsson,Claes M Gustafsson

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-REXO2 antibody

Immunoprecipitation - Anti-REXO2 antibody (AB206694)

Western blot - Anti-REXO2 antibody (AB206694)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise