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AB224547

Anti-REXO2 antibody

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(1 Publication)

Rabbit Polyclonal REXO2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human REXO2 aa 150 to C-terminus.

View Alternative Names

SFN, SMFN, CGI-114, REXO2, RNA exonuclease 2 homolog, Small fragment nuclease

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-REXO2 antibody (AB224547)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-REXO2 antibody (AB224547)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for REXO2 (green) using ab224547 at 4 μg/ml in ICC/IF.

Western blot - Anti-REXO2 antibody (AB224547)
  • WB

Supplier Data

Western blot - Anti-REXO2 antibody (AB224547)

All lanes:

Western blot - Anti-REXO2 antibody (ab224547) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) cell lysate

Predicted band size: 27 kDa

false

Western blot - Anti-REXO2 antibody (AB224547)
  • WB

Supplier Data

Western blot - Anti-REXO2 antibody (AB224547)

All lanes:

Western blot - Anti-REXO2 antibody (ab224547) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 27 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REXO2 antibody (AB224547)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REXO2 antibody (AB224547)

Paraffin-embedded human stomach tissue stained for REXO2 using ab224547 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human REXO2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3B8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REXO2 also referred to as RNase R or Oligoribonuclease is an enzyme with a molecular mass of around 26 kDa. It plays a significant role in the final steps of RNA decay. Mechanically REXO2 functions as an oligoribonuclease catalyzing the degradation of small ribonucleic acids into mononucleotides. This protein is primarily expressed in the mitochondria of the cell where it ensures the proper completion of RNA turnover.
Biological function summary

The enzyme facilitates the maintenance of cellular RNA quality. REXO2 is not a part of any larger complex but its exonuclease activity allows cells to eliminate short RNA fragments which could otherwise accumulate and lead to cellular dysfunction. Efficient removal of these fragments is essential for normal mitochondrial function and energy production within the cell.

Pathways

This enzyme is key to ensuring RNA metabolic balance. It plays a role in the RNA degradation pathway helping to process and clear unnecessary oligoribonucleotides. While REXO2 does not directly interact with other proteins in a complex it works alongside proteins such as PNPase and RNAse II which facilitate different stages of RNA degradation and processing within the cell.

REXO2 has implications in mitochondrial pathologies. Deficiencies or mutations in this enzyme can contribute to mitochondrial disorders which involve proteins such as POLG and Twinkle. Additionally its efficient RNA clearing function ties it to studies on neurodegenerative diseases where aberrant RNA processing can occur. Understanding these connections allows for deeper insight into how deviations in RNA management can translate into disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

3'-to-5'exoribonuclease that preferentially degrades DNA and RNA oligonucleotides composed of only two nucleotides (PubMed : 23741365, PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Binds and degrades longer oligonucleotides with a lower affinity (PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Plays dual roles in mitochondria, scavenging nanoRNAs (small RNA oligonucleotides of <5 nucleotides) that are produced by the degradosome and clearing short RNAs that are generated by RNA processing (PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Essential for correct initiation of mitochondrial transcription, degrading mitochondrial RNA dinucleotides to prevent RNA-primed transcription at non-canonical sites in the mitochondrial genome (PubMed : 31588022). Essential for embryonic development (By similarity).. Isoform 3. 3'-to-5'exoribonuclease that preferentially degrades DNA and RNA oligonucleotides composed of only two nucleotides.
See full target information REXO2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed38099650

2023

Distinct states of nucleolar stress induced by anticancer drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara A Potapova,Jay R Unruh,Juliana Conkright-Fincham,Charles A S Banks,Laurence Florens,David Alan Schneider,Jennifer L Gerton
View all publications
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Product promise

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