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AB221198

Anti-Rfa1/Rpa1 antibody

4

(1 Review)

|

(5 Publications)

Rabbit Polyclonal RPA70 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RFA1.

View Alternative Names

BUF2, RPA1, YAR007C, FUN3, RFA1, Replication factor A protein 1, RF-A protein 1, DNA-binding protein BUF2, Replication protein A 69 kDa DNA-binding subunit, Single-stranded DNA-binding protein

1 Images
Western blot - Anti-Rfa1/Rpa1 antibody (AB221198)
  • WB

Supplier Data

Western blot - Anti-Rfa1/Rpa1 antibody (AB221198)

SDS-PAGE 10-20% gradient gel.

All lanes:

Western blot - Anti-Rfa1/Rpa1 antibody (ab221198) at 1/2000 dilution

All lanes:

Saccharomyces cerevisiae whole cell extract (strain BY4741) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RFA1. The exact immunogen used to generate this antibody is proprietary information.

P22336

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rfa1 also known as Rpa1 is a protein component of the Replication Protein A (RPA) complex. It is 70 kDa in size and it plays an important role in DNA metabolism. This protein helps in the stabilization of single-stranded DNA intermediates. Rfa1/Rpa1 expression occurs in various tissues across different species reflecting its importance in cellular processes involving DNA.
Biological function summary

Replication Protein A helps maintain genome stability by providing a platform for the recruitment of other proteins involved in DNA repair and replication. Rpa1 is an important component of the heterotrimeric RPA complex which includes Rpa2 and Rpa3. This complex binds single-stranded DNA during replication and acts as a regulator in both DNA replication and repair pathways preventing DNA from forming secondary structures and protecting it from nucleases.

Pathways

Rfa1/Rpa1 plays a significant role in the DNA damage response pathway and in DNA replication. It works alongside proteins like ATR RAD9 and RAD17 to activate the checkpoint signaling cascade following DNA damage. This ensures proper cell cycle control and DNA repair avoiding cell cycle arrest or mutations. During DNA replication it participates with proteins like DNA polymerases and RFC to ensure accurate DNA synthesis.

Rfa1/Rpa1 is linked to cancer and genomic instability disorders. Its malfunction can lead to an impaired DNA damage response contributing to oncogenesis. The protein's interaction with BRCA1 is significant in breast cancer illustrating how its pathway involvement affects tumor suppression mechanisms. Additionally its relationship with proteins like RAD51 highlights its role in maintaining genome integrity further stressing its importance in preventing pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism. Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
See full target information RFA1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40737093

2025

S-phase checkpoint protects from aberrant replication fork processing and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Iván Núñez-Martín,Lucy S Drury,María I Martínez-Jiménez,Luis Blanco,John F X Diffley,Andrés Aguilera,Belén Gómez-González

PLoS genetics 21:e1011405 PubMed40168399

2025

A Rfa1-MN-based system reveals new factors involved in the rescue of broken replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Amiama-Roig,Marta Barrientos-Moreno,Esther Cruz-Zambrano,Luz M López-Ruiz,Román González-Prieto,Gabriel Ríos-Orelogio,Félix Prado

PLoS genetics 17:e1009322 PubMed33493195

2021

Limiting DNA polymerase delta alters replication dynamics and leads to a dependence on checkpoint activation and recombination-mediated DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Natasha C Koussa,Duncan J Smith

Science advances 6:eaay2669 PubMed32083180

2020

Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous recombination.

Applications

Unspecified application

Species

Unspecified reactive species

Gyubum Lim,Yeonji Chang,Won-Ki Huh

Nature communications 10:3515 PubMed31383866

2019

The Rad51 paralogs facilitate a novel DNA strand specific damage tolerance pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Joel C Rosenbaum,Braulio Bonilla,Sarah R Hengel,Tony M Mertz,Benjamin W Herken,Hinke G Kazemier,Catherine A Pressimone,Timothy C Ratterman,Ellen MacNary,Alessio De Magis,Youngho Kwon,Stephen K Godin,Bennett Van Houten,Daniel P Normolle,Patrick Sung,Subha R Das,Katrin Paeschke,Steven A Roberts,Andrew P VanDemark,Kara A Bernstein
View all publications
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