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AB272354

Anti-RFC antibody

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(1 Publication)

Rabbit Polyclonal RFC antibody. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC19A1 aa 200-250.

View Alternative Names

FLOT1, RFC1, SLC19A1, Reduced folate transporter, FOLT, Cyclic dinucleotide:anion antiporter SLC19A1, Folate:anion antiporter SLC19A1, Intestinal folate carrier 1, Placental folate transporter, Reduced folate carrier protein, Reduced folate transporter 1, Solute carrier family 19 member 1, IFC-1, RFC, hRFC, RFT-1, hSLC19A1

1 Images
Western blot - Anti-RFC antibody (AB272354)
  • WB

Supplier Data

Western blot - Anti-RFC antibody (AB272354)

5-20% SDS-PAGE gel.

Blocked membrane with 5% Non-fat Milk/ TBS for 1.5 hour at room temperature.

All lanes:

Western blot - Anti-RFC antibody (ab272354) at 0.5 µg/mL

Lane 1:

Rat brain tissue lysates at 50 µg

Lane 2:

Rat ovary tissue lysates at 50 µg

Lane 3:

Mouse brain tissue lysates at 50 µg

Lane 4:

Mouse testicular tissue lysates at 50 µg

Lane 5:

Mouse ovary tissue lysates at 50 µg

Lane 6:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysates at 50 µg

Lane 7:

Human placenta tissue lysates at 50 µg

Lane 8:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysates at 50 µg

Lane 9:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 50 µg

Lane 10:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysates at 50 µg

Lane 11:

22RV1 (human prostate carcinoma cell line) whole cell lysates at 50 µg

Lane 12:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysates at 50 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP at 1/10000 dilution

Predicted band size: 65 kDa

Observed band size: 65 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, African green monkey

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SLC19A1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P41440

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

AB221543

Anti-Folate Binding Protein/FBP antibody [EPR20277]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (AB221543)

  • 0
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Primary Antibodies

AB108995

Anti-Thymidylate Synthase antibody [EPR4545]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

  • 5
  • 2
Primary Antibodies

AB241949

Anti-HCP1/PCFT antibody

Immunoprecipitation - Anti-HCP1/PCFT antibody (AB241949)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.025% Sodium azide Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate, dihydrate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Reduced Folate Carrier (RFC) also known as SLC19A1 functions as a bidirectional anion exchange transporter which facilitates the transport of folate and antifolate compounds across cellular membranes. This protein has a molecular mass of approximately 65 kDa. RFC achieves high expression levels in hematopoietic tissues the placenta and the choroid plexus indicating its critical role in tissues where folate demands are significant. Additionally its distribution in various cell types suggests it plays an essential role in folate homeostasis across the body.
Biological function summary

RFC operates as an important component of a membrane transport system that influences cellular folate uptake. It ensures an adequate supply of folate a vital nutrient for one-carbon metabolism linked to DNA synthesis repair and methylation processes. RFC functions independently not as a part of any larger protein complex but interacts with other cellular systems to maintain folate levels. Its activity significantly affects cellular proliferation because folate acts as a donor of methyl groups necessary for thymidine and purine biosynthesis critical precursors for DNA production.

Pathways

Folate transport by RFC supports essential pathways such as the tetrahydrofolate cycle and the methionine cycle. These pathways are indispensable for the synthesis of nucleotides and the regeneration of homocysteine to methionine impacting processes like DNA methylation and gene expression. In these pathways RFC operates in conjunction with proteins like methionine synthase and dihydrofolate reductase which further facilitate the folate cycle showing the protein's integration into cellular metabolism.

RFC influences conditions like acute lymphoblastic leukemia (ALL) and neural tube defects (NTDs). Altered RFC function or expression can affect folate homeostasis leading to impaired DNA synthesis and repair in rapidly dividing cells which is a characteristic of ALL. Furthermore insufficient folate transport through RFC can result in inadequate neural tube closure during fetal development related to NTDs. Associations with proteins like thymidylate synthase have shown RFC's role in antifolate drug resistance emphasizing its impact on cancer therapeutics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antiporter that mediates the import of reduced folates or a subset of cyclic dinucleotides, driven by the export of organic anions (PubMed : 10787414, PubMed : 15337749, PubMed : 16115875, PubMed : 22554803, PubMed : 31126740, PubMed : 31511694, PubMed : 32276275, PubMed : 36071163, PubMed : 36265513, PubMed : 36575193, PubMed : 7826387, PubMed : 9041240). Acts as an importer of immunoreactive cyclic dinucleotides, such as cyclic GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol, and its linkage isomer 3'-3'-cGAMP, thus playing a role in triggering larger immune responses (PubMed : 31126740, PubMed : 31511694, PubMed : 36745868). Mechanistically, acts as a secondary active transporter, which exports intracellular organic anions down their concentration gradients to facilitate the uptake of its substrates (PubMed : 22554803, PubMed : 31126740, PubMed : 31511694). Has high affinity for N5-methyltetrahydrofolate, the predominant circulating form of folate (PubMed : 10787414, PubMed : 14609557, PubMed : 22554803, PubMed : 36071163, PubMed : 36265513, PubMed : 36575193). Also mediates the import of antifolate drug methotrexate (PubMed : 22554803, PubMed : 36071163, PubMed : 7615551, PubMed : 7641195, PubMed : 9767079). 5-amino-4-imidazolecarboxamide riboside (AICAR), when phosphorylated to AICAR monophosphate, can serve as an organic anion for antiporter activity (PubMed : 22554803).
See full target information SLC19A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer research communications 3:1524-1537 PubMed37575281

2023

Targeted Single-cell Isolation of Spontaneously Escaping Live Melanoma Cells for Comparative Transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline L E Tearle,Satya N V Arjunan,Szun S Tay,Feyza Colakoglu,James Cremasco,Matteo Golo,Maté Biro
View all publications
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