Rabbit Polyclonal RFC antibody. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC19A1 aa 200-250.
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Publications
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- Cancer research communications 3:1524-15372023Targeted Single-cell Isolation of Spontaneously Escaping Live Melanoma Cells for Comparative Transcriptomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacqueline L E Tearle,Satya N V Arjunan,Szun S Tay,Feyza Colakoglu,James Cremasco,Matteo Golo,Maté BiroPubMed 37575281
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate, dihydrate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLC19A1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link P41440
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| African green monkey | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.10000-0.50000 µg/mL | Notes - |
Species Rat | Dilution info 0.10000-0.50000 µg/mL | Notes - |
Species African green monkey | Dilution info 0.10000-0.50000 µg/mL | Notes - |
Species Human | Dilution info 0.10000-0.50000 µg/mL | Notes - |
Target data
Function
Antiporter that mediates the import of reduced folates or a subset of cyclic dinucleotides, driven by the export of organic anions (PubMed:10787414, PubMed:15337749, PubMed:16115875, PubMed:22554803, PubMed:31126740, PubMed:31511694, PubMed:32276275, PubMed:7826387, PubMed:9041240). Mechanistically, acts as a secondary active transporter, which exports intracellular organic anions down their concentration gradients to facilitate the uptake of its substrates (PubMed:22554803, PubMed:31126740, PubMed:31511694). Has high affinity for N5-methyltetrahydrofolate, the predominant circulating form of folate (PubMed:10787414, PubMed:14609557, PubMed:22554803). Also able to mediate the import of antifolate drug methotrexate (PubMed:22554803, PubMed:7615551, PubMed:7641195, PubMed:9767079). Also acts as an importer of immunoreactive cyclic dinucleotides, such as cyclic GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol, and its linkage isomer 3'-3'-cGAMP, thus playing a role in triggering larger immune responses (PubMed:31126740, PubMed:31511694, PubMed:36745868). 5-amino-4-imidazolecarboxamide riboside (AICAR), when phosphorylated to AICAR monophosphate, can serve as an organic anion for antiporter activity (PubMed:22554803).
Alternative names
FLOT1, RFC1, SLC19A1, Reduced folate transporter, FOLT, Cyclic dinucleotide:anion antiporter SLC19A1, Folate:anion antiporter SLC19A1, Intestinal folate carrier 1, Placental folate transporter, Reduced folate carrier protein, Reduced folate transporter 1, Solute carrier family 19 member 1, IFC-1, RFC, hRFC, RFT-1, hSLC19A1
Recommended products
Rabbit Polyclonal RFC antibody. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC19A1 aa 200-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate, dihydrate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC19A1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link P41440
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Reduced Folate Carrier (RFC) also known as SLC19A1 functions as a bidirectional anion exchange transporter which facilitates the transport of folate and antifolate compounds across cellular membranes. This protein has a molecular mass of approximately 65 kDa. RFC achieves high expression levels in hematopoietic tissues the placenta and the choroid plexus indicating its critical role in tissues where folate demands are significant. Additionally its distribution in various cell types suggests it plays an essential role in folate homeostasis across the body.
- Biological function summary
RFC operates as an important component of a membrane transport system that influences cellular folate uptake. It ensures an adequate supply of folate a vital nutrient for one-carbon metabolism linked to DNA synthesis repair and methylation processes. RFC functions independently not as a part of any larger protein complex but interacts with other cellular systems to maintain folate levels. Its activity significantly affects cellular proliferation because folate acts as a donor of methyl groups necessary for thymidine and purine biosynthesis critical precursors for DNA production.
- Pathways
Folate transport by RFC supports essential pathways such as the tetrahydrofolate cycle and the methionine cycle. These pathways are indispensable for the synthesis of nucleotides and the regeneration of homocysteine to methionine impacting processes like DNA methylation and gene expression. In these pathways RFC operates in conjunction with proteins like methionine synthase and dihydrofolate reductase which further facilitate the folate cycle showing the protein's integration into cellular metabolism.
- Associated diseases and disorders
RFC influences conditions like acute lymphoblastic leukemia (ALL) and neural tube defects (NTDs). Altered RFC function or expression can affect folate homeostasis leading to impaired DNA synthesis and repair in rapidly dividing cells which is a characteristic of ALL. Furthermore insufficient folate transport through RFC can result in inadequate neural tube closure during fetal development related to NTDs. Associations with proteins like thymidylate synthase have shown RFC's role in antifolate drug resistance emphasizing its impact on cancer therapeutics.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-RFC antibody (ab272354)
5-20% SDS-PAGE gel.
Blocked membrane with 5% Non-fat Milk/ TBS for 1.5 hour at room temperature.
All lanes: Western blot - Anti-RFC antibody (ab272354) at 0.5 µg/mL
Lane 1: Rat brain tissue lysates at 50 µg
Lane 2: Rat ovary tissue lysates at 50 µg
Lane 3: Mouse brain tissue lysates at 50 µg
Lane 4: Mouse testicular tissue lysates at 50 µg
Lane 5: Mouse ovary tissue lysates at 50 µg
Lane 6: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysates at 50 µg
Lane 7: Human placenta tissue lysates at 50 µg
Lane 8: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysates at 50 µg
Lane 9: COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 50 µg
Lane 10: PC-3 (human prostate adenocarcinoma cell line) whole cell lysates at 50 µg
Lane 11: 22RV1 (human prostate carcinoma cell line) whole cell lysates at 50 µg
Lane 12: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysates at 50 µg
SecondaryAll lanes: Goat anti-rabbit IgG-HRP at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 65 kDa
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