AB193559

Anti-RFC1 antibody

Name: Anti-RFC1 antibody (ab193559) | Abcam
Brand: Abcam Limited
SKU: AB193559

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(3 Publications)

Rabbit Polyclonal RFC1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Xenopus laevis, Chicken, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RFC1 aa 100-350.

View Alternative Names

RFC140, RFC1, Replication factor C subunit 1, Activator 1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1, DNA-binding protein PO-GA, Replication factor C 140 kDa subunit, Replication factor C large subunit, A1 140 kDa subunit, RF-C 140 kDa subunit

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-RFC1 antibody (AB193559)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFC1 antibody (AB193559)

Immunofluorescent analysis of PFA-fixed, methanol permeabilized U2OS cells transfected with Flag-RFC1 labeling RFC1 with ab193559 at 1/2000 dilution (green). Red labeling shows Flag tag detection.

Supplier Data

Western blot - Anti-RFC1 antibody (AB193559)

Incubated with ab193559 overnight at 4°C in 5% milk TBST (Tween= 0.5%).

All lanes:

Western blot - Anti-RFC1 antibody (ab193559) at 1/2000 dilution

Lane 1:

U2OS cell lysate at 40 µg

Lane 2:

NIH3T3 cell lysate at 40 µg

Lane 3:

INS1 cell lysate at 40 µg

Lane 4:

DT40 cell lysate at 40 µg

Predicted band size: 128 kDa

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Supplier Data

Western blot - Anti-RFC1 antibody (AB193559)

Lanes 1 - 2:

Western blot - Anti-RFC1 antibody (ab193559) at 1/2000 dilution

Lanes 1 - 2:

Western blot - Anti-HAUSP / USP7 antibody (<a href='/en-us/products/primary-antibodies/hausp-usp7-antibody-ab190183'>ab190183</a>) at 1/2000 dilution

Lane 1:

Xenopus laevis crude egg extracts at 50 µg

Lane 2:

U2OS whole cell lysate at 50 µg

Predicted band size: 128 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Chicken, Human, Xenopus laevis

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RFC1 aa 100-350. The exact immunogen used to generate this antibody is proprietary information.

P35251

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Reduced Folate Carrier 1 (RFC1) also known as SLC19A1 is a membrane protein that facilitates the transport of reduced folates and antifolate drugs into the cell. It exhibits a mass of approximately 65 kDa. RFC1 functions as an integral part of the cell membrane predominantly expressed in epithelial tissues including the intestine and placenta. It acts as a folate transporter that enables cellular uptake of folates critical for DNA synthesis and repair nucleotide biosynthesis and methylation reactions.

Biological function summary

RFC1 plays a major role in maintaining intracellular concentrations of folates a necessity for cell division and growth. It is part of a broader folate transport system ensuring proper utilization of folates in metabolic processes. The protein functions alongside the Proton-Coupled Folate Transporter (PCFT) and the Folate Receptor (FR) which work in tandem yet accommodate different pH levels and folate forms.

Pathways

RFC1 is essential to the folate metabolic pathway ensuring the movement of 5-methyltetrahydrofolate a critical form of folate across cell membranes. It engages in interactions with proteins like methionine synthase which play a role in homocysteine remethylation to methionine. This pathway is indispensable to methyl group transfer reactions impacting genome stability and gene expression.

Dysfunction in RFC1 activity associates with conditions such as hereditary folate malabsorption and increased susceptibility to certain cancers. Its impaired function can lead to low folate uptake causing severe consequences in rapidly dividing cells. Evidence indicates that alterations in RFC1 activity can influence the effectiveness of antifolate chemotherapeutic agents involving proteins like thymidylate synthase that depend on folate availability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA (PubMed : 9488738). This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Can bind single- or double-stranded DNA.

See full target information RFC1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 12:1661930 PubMed41070386

2025

Different responses of luminal and glandular epithelium during mouse embryo implantation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Cui,Xiangrui Guan,Peisen Liu,Qi-Xin Xu,Juan Xie,Yaqi Jiao,Lin Jin,Peng-Chao Wang,Zhenshan Yang

Journal of ethnopharmacology 282:114646 PubMed34530095

2021

Transcriptome sequencing reveals Gastrodia elata Blume could increase the cell viability of eNPCs under hypoxic condition by improving DNA damage repair ability.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Gong,Jing Cheng,Kunshan Zhang,Yanlu Wang,Siguang Li,Yuping Luo

The Journal of cell biology 218:2113-2123 PubMed31123184

2019

XLF and H2AX function in series to promote replication fork stability.

Applications

Species

Mouse

Bo-Ruei Chen,Annabel Quinet,Andrea K Byrum,Jessica Jackson,Matteo Berti,Saravanabhavan Thangavel,Andrea L Bredemeyer,Issa Hindi,Nima Mosammaparast,Jessica K Tyler,Alessandro Vindigni,Barry P Sleckman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RFC1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RFC1 antibody (AB193559)

Western blot - Anti-RFC1 antibody (AB193559)

Western blot - Anti-RFC1 antibody (AB193559)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise