Anti-RFC1 antibody - C-terminal

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFC1 antibody - C-terminal (AB229229)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RFC1 (green) using ab229229 at 1/1000 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue). Phalloidin, a cytoskeleton marker, was labeled at 1/200 dilution (red).

• IP

Supplier Data

Immunoprecipitation - Anti-RFC1 antibody - C-terminal (AB229229)

RFC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 μg of ab229229. Western blot was performed from the immunoprecipitate using ab229229 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : HeLa whole cell extract 35 μg.
Lane 2 : 4 μg preimmune Rabbit IgG instead of ab229229 in HeLa whole cell extract.
Lane 3 : ab229229 IP in HeLa whole cell extract.

All lanes:

Immunoprecipitation - Anti-RFC1 antibody - C-terminal (ab229229)

Predicted band size: 128 kDa

false

• WB

Supplier Data

Western blot - Anti-RFC1 antibody - C-terminal (AB229229)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-RFC1 antibody - C-terminal (ab229229) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 128 kDa

false