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AB251796

Anti-RFC2 antibody

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(2 Publications)

Rabbit Polyclonal RFC2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Replication factor C subunit 2 aa 1-150.

View Alternative Names

Replication factor C subunit 2, Activator 1 40 kDa subunit, Activator 1 subunit 2, Replication factor C 40 kDa subunit, A1 40 kDa subunit, RF-C 40 kDa subunit, RFC40, RFC2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody (AB251796)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody (AB251796)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for RFC2 (green) using ab251796 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC2 antibody (AB251796)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC2 antibody (AB251796)

Paraffin-embedded human vulva/anal tissue stained for RFC2 using ab251796 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-RFC2 antibody (AB251796)
  • WB

Unknown

Western blot - Anti-RFC2 antibody (AB251796)

All lanes:

Western blot - Anti-RFC2 antibody (ab251796) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 39 kDa

false

Western blot - Anti-RFC2 antibody (AB251796)
  • WB

Unknown

Western blot - Anti-RFC2 antibody (AB251796)

All lanes:

Western blot - Anti-RFC2 antibody (ab251796) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Replication factor C subunit 2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P35250

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Replication Factor C subunit 2 (RFC2) also known as Activator 1 40 kDa subunit is a component of the replication factor C complex. This complex is essential for the process of DNA replication and repair. RFC2 weighs approximately 39 kDa. It interacts directly with DNA polymerase and the DNA clamp protein proliferating cell nuclear antigen (PCNA) to initiate DNA synthesis. You will commonly find RFC2 expressed in proliferating cells where it plays a part in DNA replication and cell division.
Biological function summary

RFC2 acts as a part of the replication factor C complex which functions as a DNA-dependent ATPase. The complex assists in loading the sliding clamp PCNA onto DNA strands facilitating the DNA polymerase action on the leading and lagging strands during replication. The correct function of RFC2 in this multi-protein complex ensures the high-speed and accuracy required for efficient DNA synthesis. Additionally RFC2 helps coordinate the repair of DNA damage consolidating genomic stability.

Pathways

You will find RFC2 tightly integrated into the DNA replication and cell cycle control pathways. RFC2 through its interaction with PCNA influences the progression of the S-phase in the cell cycle. Another related protein RFC1 serves a similar function in the replication factor C complex highlighting a collaboration that is critical for DNA replication machinery's efficiency and fidelity.

RFC2 holds significance due to its role in maintaining genomic stability. Aberrations in the functions of RFC2 can lead to genomic instability contributing to cancer development. Specific cancers such as colorectal cancer have shown altered expression of RFC2. RFC2 also has implications in the context of hereditary non-polyposis colorectal cancer (HNPCC) a condition often associated with mutations in DNA mismatch repair genes like MLH1. Through this disease connection the importance of RFC2 in maintaining cellular fidelity becomes clear.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA (PubMed : 9488738). This subunit binds ATP (By similarity).
See full target information Replication factor C subunit 2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:19602 PubMed37950047

2023

Identification of DNA damage response-related genes as biomarkers for castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Masashi Oshima,Ken-Ichi Takayama,Yuta Yamada,Naoki Kimura,Haruki Kume,Tetsuya Fujimura,Satoshi Inoue

BMC gastroenterology 23:353 PubMed37821801

2023

RFC2 promotes aerobic glycolysis and progression of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fuchen Lou,Mingbao Zhang
View all publications
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Product promise

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