JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB174271

Anti-RFC2 antibody [EPR12093(B)]

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Recombinant Monoclonal RFC2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Replication factor C subunit 2, Activator 1 40 kDa subunit, Activator 1 subunit 2, Replication factor C 40 kDa subunit, A1 40 kDa subunit, RF-C 40 kDa subunit, RFC40, RFC2

3 Images
Flow Cytometry (Intracellular) - Anti-RFC2 antibody [EPR12093(B)] (AB174271)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RFC2 antibody [EPR12093(B)] (AB174271)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling RFC2 with ab174271at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody [EPR12093(B)] (AB174271)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody [EPR12093(B)] (AB174271)

Immunofluorescent analysis of MCF7 cells labeling RFC2 with ab174271 at 1/100 dilution.

Western blot - Anti-RFC2 antibody [EPR12093(B)] (AB174271)
  • WB

Supplier Data

Western blot - Anti-RFC2 antibody [EPR12093(B)] (AB174271)

All lanes:

Western blot - Anti-RFC2 antibody [EPR12093(B)] (ab174271) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12093(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Replication Factor C subunit 2 (RFC2) also known as Activator 1 40 kDa subunit is a component of the replication factor C complex. This complex is essential for the process of DNA replication and repair. RFC2 weighs approximately 39 kDa. It interacts directly with DNA polymerase and the DNA clamp protein proliferating cell nuclear antigen (PCNA) to initiate DNA synthesis. You will commonly find RFC2 expressed in proliferating cells where it plays a part in DNA replication and cell division.
Biological function summary

RFC2 acts as a part of the replication factor C complex which functions as a DNA-dependent ATPase. The complex assists in loading the sliding clamp PCNA onto DNA strands facilitating the DNA polymerase action on the leading and lagging strands during replication. The correct function of RFC2 in this multi-protein complex ensures the high-speed and accuracy required for efficient DNA synthesis. Additionally RFC2 helps coordinate the repair of DNA damage consolidating genomic stability.

Pathways

You will find RFC2 tightly integrated into the DNA replication and cell cycle control pathways. RFC2 through its interaction with PCNA influences the progression of the S-phase in the cell cycle. Another related protein RFC1 serves a similar function in the replication factor C complex highlighting a collaboration that is critical for DNA replication machinery's efficiency and fidelity.

RFC2 holds significance due to its role in maintaining genomic stability. Aberrations in the functions of RFC2 can lead to genomic instability contributing to cancer development. Specific cancers such as colorectal cancer have shown altered expression of RFC2. RFC2 also has implications in the context of hereditary non-polyposis colorectal cancer (HNPCC) a condition often associated with mutations in DNA mismatch repair genes like MLH1. Through this disease connection the importance of RFC2 in maintaining cellular fidelity becomes clear.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP (By similarity).
See full target information Replication factor C subunit 2
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of microbiology and biotechnology 32:938-948 PubMed35791074

2022

CircCOL1A2 Sponges MiR-1286 to Promote Cell Invasion and Migration of Gastric Cancer by Elevating Expression of USP10 to Downregulate RFC2 Ubiquitination Level.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Li,Lixin Chai,Zujun Ding,Huabo He

Oncology letters 22:608 PubMed34188710

2021

MicroRNA-744-5p inhibits glioblastoma malignancy by suppressing replication factor C subunit 2.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Fan,Dongxiao Yao,Pengfei Yan,Xiaobing Jiang,Jie Hu

Nature communications 12:2693 PubMed33976158

2021

An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Liu,Tianyu Tao,Shihua Liu,Xia Yang,Xuwei Chen,Jiaer Liang,Ruohui Hong,Wenting Wang,Yi Yang,Xiaoyi Li,Youhong Zhang,Quanfeng Li,Shujun Liang,Haocheng Yu,Yun Wu,Xinyu Guo,Yan Lai,Xiaofan Ding,Hongyu Guan,Jueheng Wu,Xun Zhu,Jie Yuan,Jun Li,Shicheng Su,Mengfeng Li,Xiuyu Cai,Junchao Cai,Han Tian

Journal of cellular physiology 235:8319-8333 PubMed32239691

2020

RFC2, a direct target of miR-744, modulates the cell cycle and promotes the proliferation of CRC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Hu,Hengyang Shen,Jie Li,Peng Yang,Qiou Gu,Zan Fu

International journal of molecular medicine 39:622-628 PubMed28098859

2017

DNA damage response defect in Williams-Beuren syndrome.

Applications

WB

Species

Unspecified reactive species

David Guenat,Giuseppe Merla,Eric Deconinck,Christophe Borg,Pierre-Simon Rohrlich
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32064

Anti-Cleaved PARP1 antibody [E51]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (AB32064)

  • 4
  • 18
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB29

Anti-PCNA antibody [PC10]

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PCNA antibody [PC10] (AB29)

  • 5
  • 72

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com