Rabbit Polyclonal RFC3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Replication factor C subunit 3 aa 50 to C-terminus.
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Publications
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- EMBO reports 21:e506622020FAM111 protease activity undermines cellular fitness and is amplified by gain-of-function mutations in human disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaskia Hoffmann et. al.PubMed 32776417
- Proceedings of the National Academy of Sciences of the United States of America 114:3720-37252017Triad of human cellular proteins, IRF2, FAM111A, and RFC3, restrict replication of orthopoxvirus SPI-1 host-range mutants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDebasis Panda,Daniel J Fernandez,Madhu Lal,Eugen Buehler,Bernard MossPubMed 28320935
- Translational oncology 10:1-92016Up-Regulation of RFC3 Promotes Triple Negative Breast Cancer Metastasis and is Associated With Poor Prognosis Via EMT.Applications:WB, IHC-PReactive species:HumanZhen-Yu He,San-Gang Wu,Fang Peng,Qun Zhang,Ying Luo,Ming Chen,Yong BaoPubMed 27888707
- International journal of clinical and experimental 8:8968-752015ShRNA-mediated silencing of the RFC3 gene suppress ovarian tumor cells proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuimin Shen et. al.PubMed 26464638
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Replication factor C subunit 3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P40938
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
Alternative names
Replication factor C subunit 3, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C 38 kDa subunit, A1 38 kDa subunit, RF-C 38 kDa subunit, RFC38, RFC3
Recommended products
Rabbit Polyclonal RFC3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Replication factor C subunit 3 aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Replication factor C subunit 3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P40938
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Replication Factor C subunit 3 (RFC3) also known as RFC38 or RFC37 plays an important role in DNA replication and repair by forming part of the replication factor C complex. RFC3 is a protein with a molecular mass of approximately 38 kDa. It is ubiquitously expressed in various tissues where it contributes to maintaining genomic integrity.
- Biological function summary
RFC3 facilitates the loading of the proliferating cell nuclear antigen (PCNA) onto DNA during the S phase of the cell cycle. As a component of the replication factor C (RFC) complex which is essential for DNA replication RFC3 works with other subunits to ensure the accurate duplication of the genome. It is important for the coordination and smooth progress of DNA synthesis which is vital for cell division and growth.
- Pathways
RFC3 holds a significant role in the DNA replication and cell cycle pathways. By interacting with proteins like PCNA RFC3 helps initiate and regulate the proper progression through the cell cycle checkpoints. The RFC complex which includes RFC3 is involved in tethering DNA polymerases to template DNA facilitating continuous DNA synthesis. This important interaction ensures the fidelity and efficiency of the DNA replication process.
- Associated diseases and disorders
Alterations in RFC3 expression or function can relate to cancer and genomic instability disorders. Elevated levels of RFC3 have been observed in various types of cancer where it often links with other overexpressed proteins like PCNA contributing to uncontrolled cell proliferation. Investigating RFC3's role and its interactions with related proteins helps in understanding tumorigenesis and developing potential therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC3 antibody (ab154899)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue labeling RFC3 with ab154899 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody (ab154899)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling RFC3 with ab154899 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Western blot - Anti-RFC3 antibody (ab154899)
10% SDS PAGE
All lanes: Western blot - Anti-RFC3 antibody (ab154899) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 33 kDa, 41 kDa
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