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AB154899

Anti-RFC3 antibody

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(4 Publications)

Rabbit Polyclonal RFC3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Replication factor C subunit 3 aa 50 to C-terminus.

View Alternative Names

Replication factor C subunit 3, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C 38 kDa subunit, A1 38 kDa subunit, RF-C 38 kDa subunit, RFC38, RFC3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC3 antibody (AB154899)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC3 antibody (AB154899)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue labeling RFC3 with ab154899 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody (AB154899)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody (AB154899)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling RFC3 with ab154899 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Western blot - Anti-RFC3 antibody (AB154899)
  • WB

Unknown

Western blot - Anti-RFC3 antibody (AB154899)

10% SDS PAGE

All lanes:

Western blot - Anti-RFC3 antibody (ab154899) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 33 kDa,41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Replication factor C subunit 3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P40938

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Replication Factor C subunit 3 (RFC3) also known as RFC38 or RFC37 plays an important role in DNA replication and repair by forming part of the replication factor C complex. RFC3 is a protein with a molecular mass of approximately 38 kDa. It is ubiquitously expressed in various tissues where it contributes to maintaining genomic integrity.
Biological function summary

RFC3 facilitates the loading of the proliferating cell nuclear antigen (PCNA) onto DNA during the S phase of the cell cycle. As a component of the replication factor C (RFC) complex which is essential for DNA replication RFC3 works with other subunits to ensure the accurate duplication of the genome. It is important for the coordination and smooth progress of DNA synthesis which is vital for cell division and growth.

Pathways

RFC3 holds a significant role in the DNA replication and cell cycle pathways. By interacting with proteins like PCNA RFC3 helps initiate and regulate the proper progression through the cell cycle checkpoints. The RFC complex which includes RFC3 is involved in tethering DNA polymerases to template DNA facilitating continuous DNA synthesis. This important interaction ensures the fidelity and efficiency of the DNA replication process.

Alterations in RFC3 expression or function can relate to cancer and genomic instability disorders. Elevated levels of RFC3 have been observed in various types of cancer where it often links with other overexpressed proteins like PCNA contributing to uncontrolled cell proliferation. Investigating RFC3's role and its interactions with related proteins helps in understanding tumorigenesis and developing potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA.
See full target information Replication factor C subunit 3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 21:e50662 PubMed32776417

2020

FAM111 protease activity undermines cellular fitness and is amplified by gain-of-function mutations in human disease.

Applications

Unspecified application

Species

Unspecified reactive species

Saskia Hoffmann,Satyakrishna Pentakota,Andreas Mund,Peter Haahr,Fabian Coscia,Marta Gallo,Matthias Mann,Nicholas Mi Taylor,Niels Mailand

Proceedings of the National Academy of Sciences of the United States of America 114:3720-3725 PubMed28320935

2017

Triad of human cellular proteins, IRF2, FAM111A, and RFC3, restrict replication of orthopoxvirus SPI-1 host-range mutants.

Applications

Unspecified application

Species

Unspecified reactive species

Debasis Panda,Daniel J Fernandez,Madhu Lal,Eugen Buehler,Bernard Moss

Translational oncology 10:1-9 PubMed27888707

2016

Up-Regulation of RFC3 Promotes Triple Negative Breast Cancer Metastasis and is Associated With Poor Prognosis Via EMT.

Applications

WB, IHC-P

Species

Human, Human

Zhen-Yu He,San-Gang Wu,Fang Peng,Qun Zhang,Ying Luo,Ming Chen,Yong Bao

International journal of clinical and experimental 8:8968-75 PubMed26464638

2015

ShRNA-mediated silencing of the RFC3 gene suppress ovarian tumor cells proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Shen,Juan Xu,Shanshan Zhao,Haijuan Shi,Shuzhong Yao,Nan Jiang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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