AB182143

Anti-RFC3 antibody [EPR14214]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal RFC3 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Replication factor C subunit 3, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C 38 kDa subunit, A1 38 kDa subunit, RF-C 38 kDa subunit, RFC38, RFC3

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody [EPR14214] (AB182143)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody [EPR14214] (AB182143)

Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling RFC3 using ab182143 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

Flow Cytometry (Intracellular) - Anti-RFC3 antibody [EPR14214] (AB182143)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RFC3 antibody [EPR14214] (AB182143)

Intracellular Flow Cytometry analysis of HeLa cells labeling RFC3 using ab182143 at a 1/250 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde

Supplier Data

Western blot - Anti-RFC3 antibody [EPR14214] (AB182143)

All lanes:

Western blot - Anti-RFC3 antibody [EPR14214] (ab182143) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

Carrier free

Anti-RFC3 antibody [EPR14214] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14214

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/250", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Replication Factor C subunit 3 (RFC3) also known as RFC38 or RFC37 plays an important role in DNA replication and repair by forming part of the replication factor C complex. RFC3 is a protein with a molecular mass of approximately 38 kDa. It is ubiquitously expressed in various tissues where it contributes to maintaining genomic integrity.

Biological function summary

RFC3 facilitates the loading of the proliferating cell nuclear antigen (PCNA) onto DNA during the S phase of the cell cycle. As a component of the replication factor C (RFC) complex which is essential for DNA replication RFC3 works with other subunits to ensure the accurate duplication of the genome. It is important for the coordination and smooth progress of DNA synthesis which is vital for cell division and growth.

Pathways

RFC3 holds a significant role in the DNA replication and cell cycle pathways. By interacting with proteins like PCNA RFC3 helps initiate and regulate the proper progression through the cell cycle checkpoints. The RFC complex which includes RFC3 is involved in tethering DNA polymerases to template DNA facilitating continuous DNA synthesis. This important interaction ensures the fidelity and efficiency of the DNA replication process.

Alterations in RFC3 expression or function can relate to cancer and genomic instability disorders. Elevated levels of RFC3 have been observed in various types of cancer where it often links with other overexpressed proteins like PCNA contributing to uncontrolled cell proliferation. Investigating RFC3's role and its interactions with related proteins helps in understanding tumorigenesis and developing potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.

See full target information Replication factor C subunit 3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:31 PubMed40408096

2025

Single-Cell RNA Sequencing of the Primary Visual Cortex in Mice With Optic Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Deling Li,Bin Zou,Qinyuan Hu,Xinyi Zhang,Weiting Zeng,Liling Liu,Minbin Yu

Fundamental & clinical pharmacology 39:e13044 PubMed39749364

2025

Knockdown of RFC3 enhances the sensitivity of colon cancer cells to oxaliplatin by inducing ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Youyi Wu,Tingting Chen,Songsong Wu,Yiwei Huang,Fuyao Li

Experimental and therapeutic medicine 27:222 PubMed38590579

2024

RFC3 drives the proliferation, migration, invasion and angiogenesis of colorectal cancer cells by binding KIF14.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Yu,Xinxin Wu,Fang Qian,Qian Yang

Journal of cellular and molecular medicine 27:634-649 PubMed36753396

2023

Synergistic effect of PARP inhibitor and BRD4 inhibitor in multiple models of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhan Huang,Chen Liu,Lixin You,Xi Li,Gang Chen,Junpeng Fan

Journal of proteome research 19:360-370 PubMed31693373

2019

Changes to the TDP-43 and FUS Interactomes Induced by DNA Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Tetsuya Kawaguchi,Matthew G Rollins,Mahta Moinpour,Andres A Morera,Christopher C Ebmeier,William M Old,Jacob C Schwartz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RFC3 antibody [EPR14214]

Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody [EPR14214] (AB182143)

Flow Cytometry (Intracellular) - Anti-RFC3 antibody [EPR14214] (AB182143)

Western blot - Anti-RFC3 antibody [EPR14214] (AB182143)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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