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AB250585

Anti-RFC3 antibody [EPR14214] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RFC3 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Replication factor C subunit 3, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C 38 kDa subunit, A1 38 kDa subunit, RF-C 38 kDa subunit, RFC38, RFC3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)

This data was developed using ab182143, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling RFC3 using ab182143 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

Flow Cytometry (Intracellular) - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)

This data was developed using ab182143, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa cells labeling RFC3 using ab182143 at a 1/250 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde

Western blot - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)
  • WB

Supplier Data

Western blot - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)

This data was developed using ab182143, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RFC3 antibody [EPR14214] (<a href='/en-us/products/primary-antibodies/rfc3-antibody-epr14214-ab182143'>ab182143</a>) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14214

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150077

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Primary Antibodies

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Primary Antibodies

AB174271

Anti-RFC2 antibody [EPR12093(B)]

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  • 0
  • 0
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250585 is the carrier-free version of ab182143.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Replication Factor C subunit 3 (RFC3) also known as RFC38 or RFC37 plays an important role in DNA replication and repair by forming part of the replication factor C complex. RFC3 is a protein with a molecular mass of approximately 38 kDa. It is ubiquitously expressed in various tissues where it contributes to maintaining genomic integrity.
Biological function summary

RFC3 facilitates the loading of the proliferating cell nuclear antigen (PCNA) onto DNA during the S phase of the cell cycle. As a component of the replication factor C (RFC) complex which is essential for DNA replication RFC3 works with other subunits to ensure the accurate duplication of the genome. It is important for the coordination and smooth progress of DNA synthesis which is vital for cell division and growth.

Pathways

RFC3 holds a significant role in the DNA replication and cell cycle pathways. By interacting with proteins like PCNA RFC3 helps initiate and regulate the proper progression through the cell cycle checkpoints. The RFC complex which includes RFC3 is involved in tethering DNA polymerases to template DNA facilitating continuous DNA synthesis. This important interaction ensures the fidelity and efficiency of the DNA replication process.

Alterations in RFC3 expression or function can relate to cancer and genomic instability disorders. Elevated levels of RFC3 have been observed in various types of cancer where it often links with other overexpressed proteins like PCNA contributing to uncontrolled cell proliferation. Investigating RFC3's role and its interactions with related proteins helps in understanding tumorigenesis and developing potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA.
See full target information Replication factor C subunit 3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com