Rabbit Monoclonal RFC4 antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
IP | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Rat | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.
Replication factor C subunit 4, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C 37 kDa subunit, A1 37 kDa subunit, RF-C 37 kDa subunit, RFC37, RFC4
Rabbit Monoclonal RFC4 antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EP14557
Affinity purification Protein A
Blue Ice
+4°C
+4°C
ab284847 is the carrier-free version of Anti-RFC4 antibody [EP14557] - C-terminal ab192021
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
The replication factor C subunit 4 also known as RFC4 is an essential protein in DNA replication possessing a molecular weight of approximately 37 kDa. RFC4 forms part of the larger replication factor C (RFC) complex which functions as a clamp loader for the proliferating cell nuclear antigen (PCNA). This activity facilitates the attachment of PCNA to the DNA promoting DNA polymerase processivity during replication. RFC4 is widely expressed across various tissues with higher levels noted in proliferating cells.
RFC4 plays a critical role in maintaining genomic stability and facilitating proper cell cycle progression. As a component of the RFC complex it cooperates with additional subunits to engage with DNA replication machinery effectively. This complex recognizes the primer-template junction and recruits PCNA to assist DNA polymerase in duplicating the genome accurately. RFC4's role does not end with replication as the RFC complex also partakes in DNA repair mechanisms needed for correcting replication errors.
RFC4 is actively involved in the DNA replication pathway and the DNA damage response pathway. Within these pathways it interacts with proteins like DNA polymerase delta and epsilon ensuring their efficient function during DNA synthesis and repair. RFC4 collaborates closely with PCNA in these pathways to maintain fidelity in replication and in responding to DNA damage highlighting its functional importance in genetic information preservation.
RFC4 holds significance in the context of cancer and genomic instability disorders. Dysregulation or mutations in RFC4 can lead to defective DNA replication and repair contributing to uncontrolled cell proliferation seen in cancer. Its interaction with other proteins such as PCNA and DNA polymerases can become aberrant further exacerbating disease progression. Research into RFC4 offers potential insights into therapeutic strategies that target replication and repair pathways for disease intervention.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-RFC4 antibody [EP14557] - C-terminal ab192021, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (Anti-RFC4 antibody [EP14557] - C-terminal ab192021) at 1/1000 dilution
All lanes: HeLa cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 40 kDa
This data was developed using Anti-RFC4 antibody [EP14557] - C-terminal ab192021, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (Anti-RFC4 antibody [EP14557] - C-terminal ab192021) at 1/1000 dilution
Lane 1: 293T cell lysate at 20 µg
Lane 2: K562 cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 40 kDa
This data was developed using Anti-RFC4 antibody [EP14557] - C-terminal ab192021, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (Anti-RFC4 antibody [EP14557] - C-terminal ab192021) at 1/1000 dilution
Lane 1: C6 cell lysate at 1/20 dilution
Lane 2: PC12 cell lysate at 1/20 dilution
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 40 kDa
This data was developed using Anti-RFC4 antibody [EP14557] - C-terminal ab192021, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HeLa cell lysate labeling RFC4 with Anti-RFC4 antibody [EP14557] - C-terminal ab192021 at 1/50 dilution (lane 1) or PBS control (lane 2). Secondary staining was with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.
All lanes: Immunoprecipitation - Anti-RFC4 antibody [EP14557] - C-terminal (Anti-RFC4 antibody [EP14557] - C-terminal ab192021)
Predicted band size: 40 kDa
This data was developed using Anti-RFC4 antibody [EP14557] - C-terminal ab192021, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of MCF7 cells labeling RFC4 using Anti-RFC4 antibody [EP14557] - C-terminal ab192021 at 1/100 dilution (7.4 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI.
For the 2 negative control images, Anti-RFC4 antibody [EP14557] - C-terminal ab192021 was used at 1/100 dilution, followed by Goat anti Mouse IgG (Alexa Fluor®594) at 1/500 dilution as secondary antibody.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com