Anti-RFC4 antibody [EP14557] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EP14557] - BSA and Azide free (AB284847)

This data was developed using ab192021, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of MCF7 cells labeling RFC4 using ab192021 at 1/100 dilution (7.4 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain : DAPI.

For the 2 negative control images, ab192021 was used at 1/100 dilution, followed by Goat anti Mouse IgG (Alexa Fluor®594) at 1/500 dilution as secondary antibody.

• IP

Supplier Data

Immunoprecipitation - Anti-RFC4 antibody [EP14557] - BSA and Azide free (AB284847)

This data was developed using ab192021, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HeLa cell lysate labeling RFC4 with ab192021 at 1/50 dilution (lane 1) or PBS control (lane 2). Secondary staining was with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-RFC4 antibody [EP14557] - C-terminal (<a href='/en-us/products/primary-antibodies/rfc4-antibody-ep14557-c-terminal-ab192021'>ab192021</a>)

Predicted band size: 40 kDa

false

• WB

Supplier Data

Western blot - Anti-RFC4 antibody [EP14557] - BSA and Azide free (AB284847)

This data was developed using ab192021, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (<a href='/en-us/products/primary-antibodies/rfc4-antibody-ep14557-c-terminal-ab192021'>ab192021</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 40 kDa

false

• WB

Supplier Data

Western blot - Anti-RFC4 antibody [EP14557] - BSA and Azide free (AB284847)

This data was developed using ab192021, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (<a href='/en-us/products/primary-antibodies/rfc4-antibody-ep14557-c-terminal-ab192021'>ab192021</a>) at 1/1000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 40 kDa

false

• WB

Supplier Data

Western blot - Anti-RFC4 antibody [EP14557] - BSA and Azide free (AB284847)

This data was developed using ab192021, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (<a href='/en-us/products/primary-antibodies/rfc4-antibody-ep14557-c-terminal-ab192021'>ab192021</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 1/20 dilution

Lane 2:

PC12 cell lysate at 1/20 dilution

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 40 kDa

false