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AB192021

Anti-RFC4 antibody [EP14557] - C-terminal

5

(1 Review)

|

(2 Publications)

Rabbit Monoclonal RFC4 antibody. C-terminal. Suitable for IP, WB, ICC/IF and reacts with Human, Rat samples. Cited in 2 publications.

View Alternative Names

Replication factor C subunit 4, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C 37 kDa subunit, A1 37 kDa subunit, RF-C 37 kDa subunit, RFC37, RFC4

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021)

Immunofluorescence analysis of MCF7 cells labeling RFC4 using ab192021 at 1/100 dilution (7.4 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain : DAPI.

For the 2 negative control images, ab192021 was used at 1/100 dilution, followed by Goat anti Mouse IgG (Alexa Fluor®594) at 1/500 dilution as secondary antibody.

Immunoprecipitation - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021)
  • IP

Supplier Data

Immunoprecipitation - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021)

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate labeling RFC4 with ab192021 at 1/50 dilution (lane 1) or PBS control (lane 2). Secondary staining was with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)

Predicted band size: 40 kDa

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Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021)
  • WB

Supplier Data

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021)

All lanes:

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 40 kDa

false

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021)
  • WB

Supplier Data

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021)

All lanes:

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 40 kDa

false

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021)
  • WB

Supplier Data

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021)

All lanes:

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilution

Lane 1:

C6 cell lysate at 1/20 dilution

Lane 2:

PC12 cell lysate at 1/20 dilution

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 40 kDa

false

  • Carrier free

    Anti-RFC4 antibody [EP14557] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP14557

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The replication factor C subunit 4 also known as RFC4 is an essential protein in DNA replication possessing a molecular weight of approximately 37 kDa. RFC4 forms part of the larger replication factor C (RFC) complex which functions as a clamp loader for the proliferating cell nuclear antigen (PCNA). This activity facilitates the attachment of PCNA to the DNA promoting DNA polymerase processivity during replication. RFC4 is widely expressed across various tissues with higher levels noted in proliferating cells.
Biological function summary

RFC4 plays a critical role in maintaining genomic stability and facilitating proper cell cycle progression. As a component of the RFC complex it cooperates with additional subunits to engage with DNA replication machinery effectively. This complex recognizes the primer-template junction and recruits PCNA to assist DNA polymerase in duplicating the genome accurately. RFC4's role does not end with replication as the RFC complex also partakes in DNA repair mechanisms needed for correcting replication errors.

Pathways

RFC4 is actively involved in the DNA replication pathway and the DNA damage response pathway. Within these pathways it interacts with proteins like DNA polymerase delta and epsilon ensuring their efficient function during DNA synthesis and repair. RFC4 collaborates closely with PCNA in these pathways to maintain fidelity in replication and in responding to DNA damage highlighting its functional importance in genetic information preservation.

RFC4 holds significance in the context of cancer and genomic instability disorders. Dysregulation or mutations in RFC4 can lead to defective DNA replication and repair contributing to uncontrolled cell proliferation seen in cancer. Its interaction with other proteins such as PCNA and DNA polymerases can become aberrant further exacerbating disease progression. Research into RFC4 offers potential insights into therapeutic strategies that target replication and repair pathways for disease intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.
See full target information Replication factor C subunit 4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 16:4666-4677 PubMed39144363

2024

Bioinformatic analysis of differentially expressed genes in lung cancer bone metastasis and their implications for disease progression in lung cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaojun Hong,Haiyan Hu,Dandan Liu,Xiaojian Hu,Zhanggui Wang,Daoping Zhou

Cell reports 28:3497-3509.e4 PubMed31553917

2019

Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah R Wessel,Kareem N Mohni,Jessica W Luzwick,Huzefa Dungrawala,David Cortez
View all publications
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Product promise

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