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Rabbit Monoclonal RFC4 antibody. C-terminal. Suitable for IP, WB, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication.


Images

Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021), expandable thumbnail
  • Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021), expandable thumbnail
  • Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021), expandable thumbnail
  • Immunoprecipitation - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000.00000 - 1/10000.00000
Notes

-

Species
Human
Dilution info
1/1000.00000 - 1/10000.00000
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.

Alternative names

Replication factor C subunit 4, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C 37 kDa subunit, A1 37 kDa subunit, RF-C 37 kDa subunit, RFC37, RFC4

Recommended products

Rabbit Monoclonal RFC4 antibody. C-terminal. Suitable for IP, WB, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP14557
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The replication factor C subunit 4 also known as RFC4 is an essential protein in DNA replication possessing a molecular weight of approximately 37 kDa. RFC4 forms part of the larger replication factor C (RFC) complex which functions as a clamp loader for the proliferating cell nuclear antigen (PCNA). This activity facilitates the attachment of PCNA to the DNA promoting DNA polymerase processivity during replication. RFC4 is widely expressed across various tissues with higher levels noted in proliferating cells.

Biological function summary

RFC4 plays a critical role in maintaining genomic stability and facilitating proper cell cycle progression. As a component of the RFC complex it cooperates with additional subunits to engage with DNA replication machinery effectively. This complex recognizes the primer-template junction and recruits PCNA to assist DNA polymerase in duplicating the genome accurately. RFC4's role does not end with replication as the RFC complex also partakes in DNA repair mechanisms needed for correcting replication errors.

Pathways

RFC4 is actively involved in the DNA replication pathway and the DNA damage response pathway. Within these pathways it interacts with proteins like DNA polymerase delta and epsilon ensuring their efficient function during DNA synthesis and repair. RFC4 collaborates closely with PCNA in these pathways to maintain fidelity in replication and in responding to DNA damage highlighting its functional importance in genetic information preservation.

Associated diseases and disorders

RFC4 holds significance in the context of cancer and genomic instability disorders. Dysregulation or mutations in RFC4 can lead to defective DNA replication and repair contributing to uncontrolled cell proliferation seen in cancer. Its interaction with other proteins such as PCNA and DNA polymerases can become aberrant further exacerbating disease progression. Research into RFC4 offers potential insights into therapeutic strategies that target replication and repair pathways for disease intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021), expandable thumbnail

    Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)

    All lanes: Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilution

    All lanes: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa

  • Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021), expandable thumbnail

    Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)

    All lanes: Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilution

    Lane 1: 293T cell lysate at 20 µg

    Lane 2: K562 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa

  • Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021), expandable thumbnail

    Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)

    All lanes: Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilution

    Lane 1: C6 cell lysate at 1/20 dilution

    Lane 2: PC12 cell lysate at 1/20 dilution

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa

  • Immunoprecipitation - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021), expandable thumbnail

    Immunoprecipitation - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)

    Western blot analysis of immunoprecipitation pellet from HeLa cell lysate labeling RFC4 with ab192021 at 1/50 dilution (lane 1) or PBS control (lane 2). Secondary staining was with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)

    Predicted band size: 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)

    Immunofluorescence analysis of MCF7 cells labeling RFC4 using ab192021 at 1/100 dilution (7.4 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI.

    For the 2 negative control images, ab192021 was used at 1/100 dilution, followed by Goat anti Mouse IgG (Alexa Fluor®594) at 1/500 dilution as secondary antibody.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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