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Rabbit Recombinant Monoclonal RFC4 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

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Images

Immunoprecipitation - Anti-RFC4 antibody [EPR14556] (AB182145), expandable thumbnail
  • Western blot - Anti-RFC4 antibody [EPR14556] (AB182145), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EPR14556] (AB182145), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.

Alternative names

Replication factor C subunit 4, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C 37 kDa subunit, A1 37 kDa subunit, RF-C 37 kDa subunit, RFC37, RFC4

Recommended products

Rabbit Recombinant Monoclonal RFC4 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14556
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The replication factor C subunit 4 also known as RFC4 is an essential protein in DNA replication possessing a molecular weight of approximately 37 kDa. RFC4 forms part of the larger replication factor C (RFC) complex which functions as a clamp loader for the proliferating cell nuclear antigen (PCNA). This activity facilitates the attachment of PCNA to the DNA promoting DNA polymerase processivity during replication. RFC4 is widely expressed across various tissues with higher levels noted in proliferating cells.

Biological function summary

RFC4 plays a critical role in maintaining genomic stability and facilitating proper cell cycle progression. As a component of the RFC complex it cooperates with additional subunits to engage with DNA replication machinery effectively. This complex recognizes the primer-template junction and recruits PCNA to assist DNA polymerase in duplicating the genome accurately. RFC4's role does not end with replication as the RFC complex also partakes in DNA repair mechanisms needed for correcting replication errors.

Pathways

RFC4 is actively involved in the DNA replication pathway and the DNA damage response pathway. Within these pathways it interacts with proteins like DNA polymerase delta and epsilon ensuring their efficient function during DNA synthesis and repair. RFC4 collaborates closely with PCNA in these pathways to maintain fidelity in replication and in responding to DNA damage highlighting its functional importance in genetic information preservation.

Associated diseases and disorders

RFC4 holds significance in the context of cancer and genomic instability disorders. Dysregulation or mutations in RFC4 can lead to defective DNA replication and repair contributing to uncontrolled cell proliferation seen in cancer. Its interaction with other proteins such as PCNA and DNA polymerases can become aberrant further exacerbating disease progression. Research into RFC4 offers potential insights into therapeutic strategies that target replication and repair pathways for disease intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-RFC4 antibody [EPR14556] (ab182145), expandable thumbnail

    Immunoprecipitation - Anti-RFC4 antibody [EPR14556] (ab182145)

    Detection of Human RFC4 by Western Blot of Immunoprecipitate. HeLa whole cell lysate loaded, ab182145 used for blotting immunoprecipitated RFC4 at a 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-RFC4 antibody [EPR14556] (ab182145)

    Predicted band size: 40 kDa

  • Western blot - Anti-RFC4 antibody [EPR14556] (ab182145), expandable thumbnail

    Western blot - Anti-RFC4 antibody [EPR14556] (ab182145)

    All lanes: Western blot - Anti-RFC4 antibody [EPR14556] (ab182145) at 1/10000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: HepG2 cell lysate at 20 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

    Lane 4: K562 lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

    Predicted band size: 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EPR14556] (ab182145), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EPR14556] (ab182145)

    Immunofluorescent staining of HepG2 cells(right) using anti-RFC4 Alexa Fluor® 555 RabMAB and HepG2 cells (left) using Dapi (blue)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com