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AB182145

Anti-RFC4 antibody [EPR14556]

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(4 Publications)

Rabbit Recombinant Monoclonal RFC4 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Replication factor C subunit 4, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C 37 kDa subunit, A1 37 kDa subunit, RF-C 37 kDa subunit, RFC37, RFC4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EPR14556] (AB182145)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EPR14556] (AB182145)

Immunofluorescent staining of HepG2 cells(right) using anti-RFC4 Alexa Fluor® 555 RabMAB and HepG2 cells (left) using Dapi (blue)

Immunoprecipitation - Anti-RFC4 antibody [EPR14556] (AB182145)
  • IP

Supplier Data

Immunoprecipitation - Anti-RFC4 antibody [EPR14556] (AB182145)

Detection of Human RFC4 by Western Blot of Immunoprecipitate. HeLa whole cell lysate loaded, ab182145 used for blotting immunoprecipitated RFC4 at a 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-RFC4 antibody [EPR14556] (ab182145)

Predicted band size: 40 kDa

false

Western blot - Anti-RFC4 antibody [EPR14556] (AB182145)
  • WB

Supplier Data

Western blot - Anti-RFC4 antibody [EPR14556] (AB182145)

All lanes:

Western blot - Anti-RFC4 antibody [EPR14556] (ab182145) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

Lane 4:

K562 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14556

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The replication factor C subunit 4 also known as RFC4 is an essential protein in DNA replication possessing a molecular weight of approximately 37 kDa. RFC4 forms part of the larger replication factor C (RFC) complex which functions as a clamp loader for the proliferating cell nuclear antigen (PCNA). This activity facilitates the attachment of PCNA to the DNA promoting DNA polymerase processivity during replication. RFC4 is widely expressed across various tissues with higher levels noted in proliferating cells.
Biological function summary

RFC4 plays a critical role in maintaining genomic stability and facilitating proper cell cycle progression. As a component of the RFC complex it cooperates with additional subunits to engage with DNA replication machinery effectively. This complex recognizes the primer-template junction and recruits PCNA to assist DNA polymerase in duplicating the genome accurately. RFC4's role does not end with replication as the RFC complex also partakes in DNA repair mechanisms needed for correcting replication errors.

Pathways

RFC4 is actively involved in the DNA replication pathway and the DNA damage response pathway. Within these pathways it interacts with proteins like DNA polymerase delta and epsilon ensuring their efficient function during DNA synthesis and repair. RFC4 collaborates closely with PCNA in these pathways to maintain fidelity in replication and in responding to DNA damage highlighting its functional importance in genetic information preservation.

RFC4 holds significance in the context of cancer and genomic instability disorders. Dysregulation or mutations in RFC4 can lead to defective DNA replication and repair contributing to uncontrolled cell proliferation seen in cancer. Its interaction with other proteins such as PCNA and DNA polymerases can become aberrant further exacerbating disease progression. Research into RFC4 offers potential insights into therapeutic strategies that target replication and repair pathways for disease intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA. The RFC4 subunit probably functions as a scaffold on which the other complex components can assemble.
See full target information Replication factor C subunit 4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10496 PubMed39627214

2024

ATAD5-BAZ1B interaction modulates PCNA ubiquitination during DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yeongjae Kim,Na Young Ha,Mi-Sun Kang,Eunjin Ryu,Geunil Yi,Juyeong Yoo,Nalae Kang,Byung-Gyu Kim,Kyungjae Myung,Sukhyun Kang

Nature communications 10:2420 PubMed31160570

2019

Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Sun Kang,Eunjin Ryu,Seung-Won Lee,Jieun Park,Na Young Ha,Jae Sun Ra,Yeong Jae Kim,Jinwoo Kim,Mohamed Abdel-Rahman,Su Hyung Park,Kyoo-Young Lee,Hajin Kim,Sukhyun Kang,Kyungjae Myung

Cell death & disease 9:259 PubMed29449545

2018

Transcriptional repression of DNA repair genes is a hallmark and a cause of cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Collin,Anda Huna,Marine Warnier,Jean-Michel Flaman,David Bernard

Journal of translational medicine 12:320 PubMed25407051

2014

Levels of human replication factor C4, a clamp loader, correlate with tumor progression and predict the prognosis for colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Xiang,Lekun Fang,Yanxin Luo,Zuli Yang,Yi Liao,Ji Cui,Meijin Huang,Zihuan Yang,Yan Huang,Xinjuan Fan,Huashe Wang,Lei Wang,Junsheng Peng,Jianping Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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