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Mouse Monoclonal RFC4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Dog, Rat, African green monkey, Transfected cell lysate - Human, Transfected cell line - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Replication factor C subunit 4.

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Images

Western blot - Anti-RFC4 antibody [OTI1A8] (AB156780), expandable thumbnail
  • Western blot - Anti-RFC4 antibody [OTI1A8] (AB156780), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (AB156780), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (AB156780), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (AB156780), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human Replication factor C subunit 4. Database link P35249

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Expected
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
African green monkey
Expected
Tested
Expected
Dog
Expected
Tested
Expected
Transfected cell line - Human
Not recommended
Not recommended
Tested
Transfected cell lysate - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/150
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Dog, Rat, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Species
Mouse
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Species
Dog
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Species
Rat
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Species
African green monkey
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human, Mouse, Dog, Rat, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.

Alternative names

Replication factor C subunit 4, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C 37 kDa subunit, A1 37 kDa subunit, RF-C 37 kDa subunit, RFC37, RFC4

Recommended products

Mouse Monoclonal RFC4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Dog, Rat, African green monkey, Transfected cell lysate - Human, Transfected cell line - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Replication factor C subunit 4.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human Replication factor C subunit 4. Database link P35249
Clone number
OTI1A8
Purification technique
Affinity purification
Concentration
Loading...
Purification notes

Purified from cell culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Clone OTI1A8 (formerly 1A8).

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The replication factor C subunit 4 also known as RFC4 is an essential protein in DNA replication possessing a molecular weight of approximately 37 kDa. RFC4 forms part of the larger replication factor C (RFC) complex which functions as a clamp loader for the proliferating cell nuclear antigen (PCNA). This activity facilitates the attachment of PCNA to the DNA promoting DNA polymerase processivity during replication. RFC4 is widely expressed across various tissues with higher levels noted in proliferating cells.

Biological function summary

RFC4 plays a critical role in maintaining genomic stability and facilitating proper cell cycle progression. As a component of the RFC complex it cooperates with additional subunits to engage with DNA replication machinery effectively. This complex recognizes the primer-template junction and recruits PCNA to assist DNA polymerase in duplicating the genome accurately. RFC4's role does not end with replication as the RFC complex also partakes in DNA repair mechanisms needed for correcting replication errors.

Pathways

RFC4 is actively involved in the DNA replication pathway and the DNA damage response pathway. Within these pathways it interacts with proteins like DNA polymerase delta and epsilon ensuring their efficient function during DNA synthesis and repair. RFC4 collaborates closely with PCNA in these pathways to maintain fidelity in replication and in responding to DNA damage highlighting its functional importance in genetic information preservation.

Associated diseases and disorders

RFC4 holds significance in the context of cancer and genomic instability disorders. Dysregulation or mutations in RFC4 can lead to defective DNA replication and repair contributing to uncontrolled cell proliferation seen in cancer. Its interaction with other proteins such as PCNA and DNA polymerases can become aberrant further exacerbating disease progression. Research into RFC4 offers potential insights into therapeutic strategies that target replication and repair pathways for disease intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-RFC4 antibody [OTI1A8] (ab156780), expandable thumbnail

    Western blot - Anti-RFC4 antibody [OTI1A8] (ab156780)

    All lanes: Western blot - Anti-RFC4 antibody [OTI1A8] (ab156780) at 1/200 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg

    Lane 2: HEK-293T cell lysate transfected with pCMV6-ENTRY RFC4 cDNA at 5 µg

    Predicted band size: 40 kDa

  • Western blot - Anti-RFC4 antibody [OTI1A8] (ab156780), expandable thumbnail

    Western blot - Anti-RFC4 antibody [OTI1A8] (ab156780)

    All lanes: Western blot - Anti-RFC4 antibody [OTI1A8] (ab156780) at 1/200 dilution

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

    Lane 3: SVT2 cell extract at 35 µg

    Lane 4: A549 (human lung carcinoma cell line) cell extract at 35 µg

    Lane 5: COS-7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg

    Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

    Lane 7: MDCK (Canine kidney cell line) cell extract at 35 µg

    Lane 8: PC12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

    Lane 9: MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (ab156780), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (ab156780)

    Paraffin-embedded human pancreas tissue stained for RFC4 using ab156780 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (ab156780), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (ab156780)

    Paraffin-embedded human pancreas carcinoma tissue stained for RFC4 using ab156780 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (ab156780), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (ab156780)

    Paraffin-embedded human tonsil tissue stained for RFC4 using ab156780 at 1/150 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [OTI1A8] (ab156780), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [OTI1A8] (ab156780)

    COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY RFC4 cDNA using ab156780 at 1/100 dilution in ICC/IF.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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