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AB156780

Anti-RFC4 antibody [OTI1A8]

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(5 Publications)

Mouse Monoclonal RFC4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Dog, Rat, African green monkey, Transfected cell lysate - Human, Transfected cell line - Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Replication factor C subunit 4.

View Alternative Names

Replication factor C subunit 4, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C 37 kDa subunit, A1 37 kDa subunit, RF-C 37 kDa subunit, RFC37, RFC4

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (AB156780)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (AB156780)

Paraffin-embedded human pancreas carcinoma tissue stained for RFC4 using ab156780 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (AB156780)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (AB156780)

Paraffin-embedded human tonsil tissue stained for RFC4 using ab156780 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (AB156780)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (AB156780)

Paraffin-embedded human pancreas tissue stained for RFC4 using ab156780 at 1/150 dilution in immunohistochemical analysis.

Western blot - Anti-RFC4 antibody [OTI1A8] (AB156780)
  • WB

Unknown

Western blot - Anti-RFC4 antibody [OTI1A8] (AB156780)

All lanes:

Western blot - Anti-RFC4 antibody [OTI1A8] (ab156780) at 1/200 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (Canine kidney cell line) cell extract at 35 µg

Lane 8:

PC12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 40 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [OTI1A8] (AB156780)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [OTI1A8] (AB156780)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY RFC4 cDNA using ab156780 at 1/100 dilution in ICC/IF.

Western blot - Anti-RFC4 antibody [OTI1A8] (AB156780)
  • WB

Unknown

Western blot - Anti-RFC4 antibody [OTI1A8] (AB156780)

All lanes:

Western blot - Anti-RFC4 antibody [OTI1A8] (ab156780) at 1/200 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY RFC4 cDNA at 5 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1A8

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Dog, Human, African green monkey

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Replication factor C subunit 4.

P35249

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Clone OTI1A8 (formerly 1A8).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The replication factor C subunit 4 also known as RFC4 is an essential protein in DNA replication possessing a molecular weight of approximately 37 kDa. RFC4 forms part of the larger replication factor C (RFC) complex which functions as a clamp loader for the proliferating cell nuclear antigen (PCNA). This activity facilitates the attachment of PCNA to the DNA promoting DNA polymerase processivity during replication. RFC4 is widely expressed across various tissues with higher levels noted in proliferating cells.
Biological function summary

RFC4 plays a critical role in maintaining genomic stability and facilitating proper cell cycle progression. As a component of the RFC complex it cooperates with additional subunits to engage with DNA replication machinery effectively. This complex recognizes the primer-template junction and recruits PCNA to assist DNA polymerase in duplicating the genome accurately. RFC4's role does not end with replication as the RFC complex also partakes in DNA repair mechanisms needed for correcting replication errors.

Pathways

RFC4 is actively involved in the DNA replication pathway and the DNA damage response pathway. Within these pathways it interacts with proteins like DNA polymerase delta and epsilon ensuring their efficient function during DNA synthesis and repair. RFC4 collaborates closely with PCNA in these pathways to maintain fidelity in replication and in responding to DNA damage highlighting its functional importance in genetic information preservation.

RFC4 holds significance in the context of cancer and genomic instability disorders. Dysregulation or mutations in RFC4 can lead to defective DNA replication and repair contributing to uncontrolled cell proliferation seen in cancer. Its interaction with other proteins such as PCNA and DNA polymerases can become aberrant further exacerbating disease progression. Research into RFC4 offers potential insights into therapeutic strategies that target replication and repair pathways for disease intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.
See full target information Replication factor C subunit 4
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 161:27 PubMed39192374

2024

The novel circFKBP8/miR-432-5p/E2F7 cascade functions as a regulatory network in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongkui Jin,Wang Xu,Kunlin Yu,Cailu Luo,Xiaodan Luo,Tao Lian,Changshan Liu

Bioscience trends 15:249-256 PubMed34053971

2021

Replication Factor C4 in human hepatocellular carcinoma: A potent prognostic factor associated with cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Chen,Yayue Liu,Xiaochen Ma,Qingli Li,Yangfan Zhang,Qingqing Xiong,Tianqiang Song

Nature communications 12:2693 PubMed33976158

2021

An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Liu,Tianyu Tao,Shihua Liu,Xia Yang,Xuwei Chen,Jiaer Liang,Ruohui Hong,Wenting Wang,Yi Yang,Xiaoyi Li,Youhong Zhang,Quanfeng Li,Shujun Liang,Haocheng Yu,Yun Wu,Xinyu Guo,Yan Lai,Xiaofan Ding,Hongyu Guan,Jueheng Wu,Xun Zhu,Jie Yuan,Jun Li,Shicheng Su,Mengfeng Li,Xiuyu Cai,Junchao Cai,Han Tian

PeerJ 8:e10386 PubMed33344075

2020

Identification of significant genes signatures and prognostic biomarkers in cervical squamous carcinoma via bioinformatic data.

Applications

Unspecified application

Species

Unspecified reactive species

Yunan He,Shunjie Hu,Jiaojiao Zhong,Anran Cheng,Nianchun Shan

British journal of cancer 118:1074-1083 PubMed29576625

2018

Targeting glutaminolysis in chondrosarcoma in context of the IDH1/2 mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth F P Peterse,Bertine Niessen,Ruben D Addie,Yvonne de Jong,Arjen H G Cleven,Alwine B Kruisselbrink,Brendy E W M van den Akker,Remco J Molenaar,Anne-Marie Cleton-Jansen,Judith V M G Bovée
View all publications
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