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AB254853

Anti-RFC5 antibody

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(1 Publication)

Rabbit Polyclonal RFC5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RFC5 aa 150-250.

View Alternative Names

Replication factor C subunit 5, Activator 1 36 kDa subunit, Activator 1 subunit 5, Replication factor C 36 kDa subunit, A1 36 kDa subunit, RF-C 36 kDa subunit, RFC36, RFC5

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RFC5 antibody (AB254853)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RFC5 antibody (AB254853)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling RFC5 using ab254853 at 4μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC5 antibody (AB254853)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC5 antibody (AB254853)

Paraffin-embedded human testis tissue stained for RFC5 using ab254853 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-RFC5 antibody (AB254853)
  • WB

Supplier Data

Western blot - Anti-RFC5 antibody (AB254853)

Lane 1:

Western blot - Anti-RFC5 antibody (ab254853)

Lanes 2 - 3:

Western blot - Anti-RFC5 antibody (ab254853) at 0.4 µg/mL

Lane 1:

MW marker

Lane 2:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 3:

U-251MG sp whole cell lysate

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RFC5 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

P40937

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RFC5 also known as Replication Factor C subunit 5 or RFC38 is a critical component of the RFC complex involved in DNA replication and repair. This protein has a molecular mass of approximately 38 kDa. It is expressed in various tissues serving an essential function in maintaining genomic stability. The RFC5 protein associates with other subunits to form a complex that acts as a clamp loader during DNA replication.
Biological function summary

RFC5 plays an important role in coordinating the proper functioning of the DNA replication machinery. It is part of the larger RFC complex which comprises five subunits working in concert to load the proliferating cell nuclear antigen (PCNA) onto DNA strands. This loading action is fundamental to the process of DNA synthesis ensuring that DNA polymerases can operate effectively. By collaborating with other components of the replication machinery RFC5 ensures accuracy and efficiency during cell division.

Pathways

RFC5 participates in DNA replication and repair pathways. It is closely linked with the DNA damage response pathway where it associates with the PCNA and other replication factors such as polymerase δ and polymerase ε. Through these interactions RFC5 helps initiate and sustain the replication fork stability and repair mechanisms important for genomic integrity.

RFC5 associates with conditions like cancer and genomic instability disorders. Mutations or dysregulation of RFC5 may contribute to uncontrolled cell proliferation in cancers. Its interaction with the PCNA and other replication factors highlights its relevance in tumorigenesis and genomic maintenance. Studying RFC5 can provide insights into potential therapeutic targets for cancers associated with replication stress and DNA repair defects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA.
See full target information RFC5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 25: PubMed35419615

2022

SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β‑catenin/TCF/LEF signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Cai,Zhenhua Sun,Lili Zhang,Hongrui Xu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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