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AB3619

Anti-RFC5 antibody

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(2 Publications)

Goat Polyclonal RFC5 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RFC5.

View Alternative Names

Replication factor C subunit 5, Activator 1 36 kDa subunit, Activator 1 subunit 5, Replication factor C 36 kDa subunit, A1 36 kDa subunit, RF-C 36 kDa subunit, RFC36, RFC5

1 Images
Western blot - Anti-RFC5 antibody (AB3619)
  • WB

Unknown

Western blot - Anti-RFC5 antibody (AB3619)

Sample : 50μg Hela cell nuclear extract

Anti-RFC5 (ab3619) used at 1 : 1000 dilution

Sample : 50μg Hela cell nuclear extract

Anti-RFC5 (ab3619) used at 1 : 1000 dilution

All lanes:

Western blot - Anti-RFC5 antibody (ab3619)

Predicted band size: 38 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human RFC5. The exact immunogen used to generate this antibody is proprietary information.

P40937

Specificity

We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RFC5 also known as Replication Factor C subunit 5 or RFC38 is a critical component of the RFC complex involved in DNA replication and repair. This protein has a molecular mass of approximately 38 kDa. It is expressed in various tissues serving an essential function in maintaining genomic stability. The RFC5 protein associates with other subunits to form a complex that acts as a clamp loader during DNA replication.
Biological function summary

RFC5 plays an important role in coordinating the proper functioning of the DNA replication machinery. It is part of the larger RFC complex which comprises five subunits working in concert to load the proliferating cell nuclear antigen (PCNA) onto DNA strands. This loading action is fundamental to the process of DNA synthesis ensuring that DNA polymerases can operate effectively. By collaborating with other components of the replication machinery RFC5 ensures accuracy and efficiency during cell division.

Pathways

RFC5 participates in DNA replication and repair pathways. It is closely linked with the DNA damage response pathway where it associates with the PCNA and other replication factors such as polymerase δ and polymerase ε. Through these interactions RFC5 helps initiate and sustain the replication fork stability and repair mechanisms important for genomic integrity.

RFC5 associates with conditions like cancer and genomic instability disorders. Mutations or dysregulation of RFC5 may contribute to uncontrolled cell proliferation in cancers. Its interaction with the PCNA and other replication factors highlights its relevance in tumorigenesis and genomic maintenance. Studying RFC5 can provide insights into potential therapeutic targets for cancers associated with replication stress and DNA repair defects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA.
See full target information RFC5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biology 30:4828-39 PubMed20713449

2010

Replication factor C recruits DNA polymerase delta to sites of nucleotide excision repair but is not required for PCNA recruitment.

Applications

ICC/IF

Species

Unspecified reactive species

René M Overmeer,Audrey M Gourdin,Ambra Giglia-Mari,Hanneke Kool,Adriaan B Houtsmuller,Gregg Siegal,Maria I Fousteri,Leon H F Mullenders,Wim Vermeulen

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51:751-60 PubMed12754286

2003

Expression of catalase mRNA and protein in adult rat brain: detection by nonradioactive in situ hybridization with signal amplification by catalyzed reporter deposition (ISH-CARD) and immunohistochemistry (IHC)/immunofluorescence (IF).

Applications

Unspecified application

Species

Unspecified reactive species

Arno Schad,H Dariush Fahimi,Alfred Völkl,Eveline Baumgart
View all publications
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Product promise

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