Goat Polyclonal RFC5 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RFC5.
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Publications
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- Molecular and cellular biology 30:4828-392010Replication factor C recruits DNA polymerase delta to sites of nucleotide excision repair but is not required for PCNA recruitment.Applications:ICC/IFReactive species:Unspecified reactive speciesRené M Overmeer et. al.PubMed 20713449
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51:751-602003Expression of catalase mRNA and protein in adult rat brain: detection by nonradioactive in situ hybridization with signal amplification by catalyzed reporter deposition (ISH-CARD) and immunohistochemistry (IHC)/immunofluorescence (IF).Applications:Unspecified applicationReactive species:Unspecified reactive speciesArno Schad,H Dariush Fahimi,Alfred Völkl,Eveline BaumgartPubMed 12754286
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RFC5. The exact immunogen used to generate this antibody is proprietary information. Database link P40937
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Chimpanzee | Predicted |
| Gorilla | Predicted |
| Orangutan | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
Alternative names
Replication factor C subunit 5, Activator 1 36 kDa subunit, Activator 1 subunit 5, Replication factor C 36 kDa subunit, A1 36 kDa subunit, RF-C 36 kDa subunit, RFC36, RFC5
Recommended products
Goat Polyclonal RFC5 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RFC5.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RFC5. The exact immunogen used to generate this antibody is proprietary information. Database link P40937
- Purification technique
- Affinity purification Immunogen
- Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RFC5 also known as Replication Factor C subunit 5 or RFC38 is a critical component of the RFC complex involved in DNA replication and repair. This protein has a molecular mass of approximately 38 kDa. It is expressed in various tissues serving an essential function in maintaining genomic stability. The RFC5 protein associates with other subunits to form a complex that acts as a clamp loader during DNA replication.
- Biological function summary
RFC5 plays an important role in coordinating the proper functioning of the DNA replication machinery. It is part of the larger RFC complex which comprises five subunits working in concert to load the proliferating cell nuclear antigen (PCNA) onto DNA strands. This loading action is fundamental to the process of DNA synthesis ensuring that DNA polymerases can operate effectively. By collaborating with other components of the replication machinery RFC5 ensures accuracy and efficiency during cell division.
- Pathways
RFC5 participates in DNA replication and repair pathways. It is closely linked with the DNA damage response pathway where it associates with the PCNA and other replication factors such as polymerase δ and polymerase ε. Through these interactions RFC5 helps initiate and sustain the replication fork stability and repair mechanisms important for genomic integrity.
- Associated diseases and disorders
RFC5 associates with conditions like cancer and genomic instability disorders. Mutations or dysregulation of RFC5 may contribute to uncontrolled cell proliferation in cancers. Its interaction with the PCNA and other replication factors highlights its relevance in tumorigenesis and genomic maintenance. Studying RFC5 can provide insights into potential therapeutic targets for cancers associated with replication stress and DNA repair defects.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-RFC5 antibody (ab3619)
Sample: 50μg Hela cell nuclear extract
Anti-RFC5 (ab3619) used at 1:1000 dilution
Sample: 50μg Hela cell nuclear extract
Anti-RFC5 (ab3619) used at 1:1000 dilution
All lanes: Western blot - Anti-RFC5 antibody (ab3619)
Predicted band size: 38 kDa
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