Anti-RFP antibody

• WB

Supplier Data

Western blot - Anti-RFP antibody (AB124754)

All lanes:

Western blot - Anti-RFP antibody (ab124754) at 1 µg/mL

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 10 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Lane 3:

CHO-K1 (chinese hamster ovary cell line) cell lysate at 10 µg

Lane 4:

MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate at 10 µg

Lane 5:

A431 (human epidermoid carcinoma cell line) cell lysate at 10 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

Lane 7:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 10 µg

Lane 8:

E-coli HCP Control at 10 µg

Lane 9:

FLAG Positive Control Lysate at 10 µg

Lane 10:

Red Fluorescent Protein at 0.05 µg

Lane 11:

Green Fluorescent Protein at 0.05 µg

Lane 12:

Glutathione-S-Transferase Protein at 0.05 µg

Lane 13:

Maltose Binding Protein at 0.05 µg

Predicted band size: 26 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFP antibody (AB124754)

Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse embryonic myocardial tissue, labelling RFP with ab124754 at a 1/50 dilution for 1 hour at room temperature. The secondary used was a a rabbit fluorescein conjugate at a 1/250 dilution for 1 hour at room temperature. Counterstain is an expression of RFP in red Tomato transgenic mice.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFP antibody (AB124754)

Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse lung sections, labelling RFP with ab124754 at 1/50 dilution for 1 hour at room temperature. The secondary used was a rabbit fluorescein conjugate at 1/250 incubated for 1 hour at room temperature.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFP antibody (AB124754)

PFA-fixed DsRed transgenic mouse retina tissue stained for RFP using ab124754 at 1/100 dilution in immunohistochemical analysis.

Blocking : 3% BSA, 0.3% Triton.

Secondary antibody : Alexa488 secondary antibody at 1 : 10,000 for 4 hours at RT.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFP antibody (AB124754)

Immunohistochemical immunofluorescence analysis of 0.5% PFA fixed Rabbit Hair folicle section labelling RFP using ab124754 at a dilution of 10 ug/mL for 1 hour at room temperature. The secondary used was a blue rabbit fluorescein conjugate at a 1/10,000 for 45 mins at room temperature. A counterstain against HOP-derived cells was used (red).

• WB

Supplier Data

Western blot - Anti-RFP antibody (AB124754)

All lanes:

Western blot - Anti-RFP antibody (ab124754) at 1/1000 dilution

Lane 1:

RFP at 0.05 µg with Blocking buffer

Lane 2:

Human IgG at 0.05 µg with Blocking buffer

Lane 3:

Goat IgG at 0.05 µg with Blocking buffer

Lane 4:

Mouse IgG at 0.05 µg with Blocking buffer

Secondary

All lanes:

Rabbit peroxidase conjugate at 1/40000 dilution

Predicted band size: 26 kDa

Observed band size: 30 kDa

false