Rabbit Polyclonal RFP antibody. Suitable for IP, WB, ICC/IF and reacts with Tag samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Sea anenome RFP_DISSP.
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
IP | WB | ICC/IF | |
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Tag | Tested | Tested | Tested |
Species | Dilution info | Notes |
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Species Tag | Dilution info 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Tag | Dilution info 1/5000 - 1/20000 | Notes - |
Species | Dilution info | Notes |
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Species Tag | Dilution info 1/5000 | Notes - |
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Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.
Red fluorescent protein drFP583, DsRed
Rabbit Polyclonal RFP antibody. Suitable for IP, WB, ICC/IF and reacts with Tag samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Sea anenome RFP_DISSP.
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
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12% SDS PAGE
All lanes: Western blot - Anti-RFP antibody (ab152123) at 1/10000 dilution
Lane 1: Non-transfected 293T whole cell lysate at 5 µg
Lane 2: GFP-transfected 293T whole cell lysate at 5 µg
Lane 3: RFP-transfected 293T whole cell lysate at 5 µg
Predicted band size: 26 kDa
Immunoprecipitation of RFP-tagged protein.
HeLa transfected lysates were subjected to immunoprecipitation using:
Lane 1: 15 μg of HeLa transfected lysate
Lane 2: normal rabbit IgG
Lane 3: 2.5 μg ab152123
Detection ab152123 at 1/10000 dilution
All lanes: Immunoprecipitation - Anti-RFP antibody (ab152123)
Predicted band size: 26 kDa
Immunofluorescence analysis of RFP-transfected HeLa cells labeling RFP with ab152123: (1) RFP is expressed in the tranfected cell, (2) The cell expressing RFP is detected using ab152123 at 1/5000 followed by Alexa Fluor 488 at 1/500 Goat anti-Rabbit IgG, (3) Merged with DNA probe, the lower cell represents a negtive control.
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