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Rabbit Polyclonal RFP antibody. Suitable for IP, WB, ICC/IF and reacts with Tag samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Sea anenome RFP_DISSP.

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Images

Western blot - Anti-RFP antibody (AB152123), expandable thumbnail
  • Immunoprecipitation - Anti-RFP antibody (AB152123), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (AB152123), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Sea anenome RFP_DISSP. Database link Q9U6Y8

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Tag
Tested
Tested
Tested

Tested
Tested

Species
Tag
Dilution info
1/10000
Notes

-

Tested
Tested

Species
Tag
Dilution info
1/5000 - 1/20000
Notes

-

Tested
Tested

Species
Tag
Dilution info
1/5000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.

Alternative names

Recommended products

Rabbit Polyclonal RFP antibody. Suitable for IP, WB, ICC/IF and reacts with Tag samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Sea anenome RFP_DISSP.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Sea anenome RFP_DISSP. Database link Q9U6Y8
Purification technique
Affinity purification Immunogen
Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-RFP antibody (ab152123), expandable thumbnail

    Western blot - Anti-RFP antibody (ab152123)

    12% SDS PAGE

    All lanes: Western blot - Anti-RFP antibody (ab152123) at 1/10000 dilution

    Lane 1: Non-transfected 293T whole cell lysate at 5 µg

    Lane 2: GFP-transfected 293T whole cell lysate at 5 µg

    Lane 3: RFP-transfected 293T whole cell lysate at 5 µg

    Predicted band size: 26 kDa

  • Immunoprecipitation - Anti-RFP antibody (ab152123), expandable thumbnail

    Immunoprecipitation - Anti-RFP antibody (ab152123)

    Immunoprecipitation of RFP-tagged protein.

    HeLa transfected lysates were subjected to immunoprecipitation using:

    Lane 1: 15 μg of HeLa transfected lysate

    Lane 2: normal rabbit IgG

    Lane 3: 2.5 μg ab152123

    Detection ab152123 at 1/10000 dilution

    All lanes: Immunoprecipitation - Anti-RFP antibody (ab152123)

    Predicted band size: 26 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (ab152123), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (ab152123)

    Immunofluorescence analysis of RFP-transfected HeLa cells labeling RFP with ab152123: (1) RFP is expressed in the tranfected cell, (2) The cell expressing RFP is detected using ab152123 at 1/5000 followed by Alexa Fluor 488 at 1/500 Goat anti-Rabbit IgG, (3) Merged with DNA probe, the lower cell represents a negtive control.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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