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AB152123

Anti-RFP antibody

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(5 Publications)

Rabbit Polyclonal RFP antibody. Suitable for IP, WB, ICC/IF and reacts with Tag samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Sea anenome Red fluorescent protein drFP583.

View Alternative Names

Red fluorescent protein drFP583, DsRed

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (AB152123)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (AB152123)

Immunofluorescence analysis of RFP-transfected HeLa cells labeling RFP with ab152123 : (1) RFP is expressed in the tranfected cell, (2) The cell expressing RFP is detected using ab152123 at 1/5000 followed by Alexa Fluor 488 at 1/500 Goat anti-Rabbit IgG, (3) Merged with DNA probe, the lower cell represents a negtive control.

Immunoprecipitation - Anti-RFP antibody (AB152123)
  • IP

Unknown

Immunoprecipitation - Anti-RFP antibody (AB152123)

Immunoprecipitation of RFP-tagged protein.
HeLa transfected lysates were subjected to immunoprecipitation using :
Lane 1 : 15 μg of HeLa transfected lysate
Lane 2 : normal rabbit IgG
Lane 3 : 2.5 μg ab152123
Detection ab152123 at 1/10000 dilution

All lanes:

Immunoprecipitation - Anti-RFP antibody (ab152123)

Predicted band size: 26 kDa

false

Western blot - Anti-RFP antibody (AB152123)
  • WB

Unknown

Western blot - Anti-RFP antibody (AB152123)

12% SDS PAGE

All lanes:

Western blot - Anti-RFP antibody (ab152123) at 1/10000 dilution

Lane 1:

Non-transfected 293T whole cell lysate at 5 µg

Lane 2:

GFP-transfected 293T whole cell lysate at 5 µg

Lane 3:

RFP-transfected 293T whole cell lysate at 5 µg

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

ICC/IF, WB, IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Sea anenome Red fluorescent protein drFP583.

Q9U6Y8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.
See full target information Red fluorescent protein drFP583
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Advanced materials (Deerfield Beach, Fla.) 34:e2110618 PubMed35119139

2022

pH-Responsive Polymer Nanoparticles for Efficient Delivery of Cas9 Ribonucleoprotein With or Without Donor DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Ruosen Xie,Xiuxiu Wang,Yuyuan Wang,Mingzhou Ye,Yi Zhao,Brian S Yandell,Shaoqin Gong

Journal of virology 95:e0015321 PubMed34287041

2021

Orf Virus ORF120 Protein Positively Regulates the NF-κB Pathway by Interacting with G3BP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlong Zhou,Jiyu Guan,Feng Gao,Zi Li,Yungang Lan,Huijun Lu,Deguang Song,Lijun Lv,Pin Lv,Mengshi Xu,Zhenzhen Wang,Hongbin He,Kui Zhao,Wenqi He

Journal of controlled release : official journal of the Controlled Release Society 336:296-309 PubMed34174352

2021

In vivo targeted delivery of nucleic acids and CRISPR genome editors enabled by GSH-responsive silica nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Yuyuan Wang,Pawan K Shahi,Xiuxiu Wang,Ruosen Xie,Yi Zhao,Min Wu,Seth Roge,Bikash R Pattnaik,Shaoqin Gong

Theranostics 10:8446-8467 PubMed32724480

2020

Lentivirus-mediated IL-10-expressing Bone Marrow Mesenchymal Stem Cells promote corneal allograft survival upregulating lncRNA 003946 in a rat model of corneal allograft rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxiao Lu,Yusha Ru,Chenchen Chu,Ying Lv,Yichen Gao,Zhe Jia,Yue Huang,Yan Zhang,Shaozhen Zhao

eLife 6: PubMed28425915

2017

Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryos.

Applications

IP

Species

Unspecified reactive species

Shai R Joseph,Máté Pálfy,Lennart Hilbert,Mukesh Kumar,Jens Karschau,Vasily Zaburdaev,Andrej Shevchenko,Nadine L Vastenhouw
View all publications
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Product promise

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