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AB28664

Anti-RFP antibody

4

(4 Reviews)

|

(15 Publications)

Rabbit Polyclonal RFP antibody. Suitable for WB, ICC/IF and reacts with Tag samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Sea anenome Red fluorescent protein drFP583 aa 1-50.

View Alternative Names

Red fluorescent protein drFP583, DsRed

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (AB28664)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (AB28664)

Immunofluorescent analysis of recombinant Red Fluorescent Protein (RFP)-transfected HeLa cells. Cells were transfected with a pCMV RFP C-Myc plasmid and 48 hours post-transfection cells were fixed with formalin and permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were blocked with 3% BSA for 15 minutes at room temperature, and then probed with ab28664 at a dilution of 1/50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight® 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

Western blot - Anti-RFP antibody (AB28664)
  • WB

Unknown

Western blot - Anti-RFP antibody (AB28664)

Western blot of recombinant RFP using ab28664.

All lanes:

Western blot - Anti-RFP antibody (ab28664)

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Sea anenome Red fluorescent protein drFP583 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9U6Y8

Specificity

ab28664 detects RFP tagged proteins. This antibody is specific for the RFP tag. It shows very low reactivity to GFP or GFP tagged proteins. ab28664 non-specifically detects a 45 kDa protein from E. coli extracts. This non-specific cross-reactivity is not present in mammalian samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Use at a concentration of 2 μg/ml. Block membrane in 5% milk and incubate primary and secondary antibodies in milk.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.
See full target information Red fluorescent protein drFP583
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:3120 PubMed38600106

2024

Salmonella exploits membrane reservoirs for invasion of host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxian Zhu,Andrew M Sydor,Kirsten C Boddy,Etienne Coyaud,Estelle M N Laurent,Aaron Au,Joel M J Tan,Bing-Ru Yan,Jason Moffat,Aleixo M Muise,Christopher M Yip,Sergio Grinstein,Brian Raught,John H Brumell

Nature communications 14:6770 PubMed37914730

2023

ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian Hirschenberger,Alice Lepelley,Ulrich Rupp,Susanne Klute,Victoria Hunszinger,Lennart Koepke,Veronika Merold,Blaise Didry-Barca,Fanny Wondany,Tim Bergner,Tatiana Moreau,Mathieu P Rodero,Reinhild Rösler,Sebastian Wiese,Stefano Volpi,Marco Gattorno,Riccardo Papa,Sally-Ann Lynch,Marte G Haug,Gunnar Houge,Kristen M Wigby,Jessica Sprague,Jerica Lenberg,Clarissa Read,Paul Walther,Jens Michaelis,Frank Kirchhoff,Carina C de Oliveira Mann,Yanick J Crow,Konstantin M J Sparrer

Journal of extracellular vesicles 12:e12322 PubMed37186457

2023

Quantitative imaging of vesicle-protein interactions reveals close cooperation among proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Minkwon Cha,Sang Hyeok Jeong,Jaehun Jung,Yoonjin Baeg,Sung-Soo Park,Seoyoon Bae,Chan Seok Lim,Jun Hyuk Park,Jie-Oh Lee,Yong Song Gho,Seung Wook Oh,Min Ju Shon

Insect biochemistry and molecular biology 119:103324 PubMed31978587

2020

Bicistronic expression and differential localization of proteins in insect cells and Drosophila suzukii using picornaviral 2A peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Schwirz,Ying Yan,Zdenek Franta,Marc F Schetelig

Cancer biology & medicine 16:729-742 PubMed31908891

2020

PADI3 induces cell cycle arrest the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotian Chang,Zhengbin Chai,Jiaorui Zou,Hongxing Wang,Yao Wang,Yabing Zheng,Hui Wu,Chunyan Liu

Cancer cell international 19:277 PubMed31708688

2019

PADI3 plays an antitumor role via the Hsp90/CKS1 pathway in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengbin Chai,Li Wang,Yabing Zheng,Na Liang,Xiwei Wang,Yingying Zheng,Zhiwei Zhang,Chuanxi Zhao,Tingting Zhu,Chunyan Liu

Genes & development 31:1679-1692 PubMed28916710

2017

APC sets the Wnt tone necessary for cerebral cortical progenitor development.

Applications

Unspecified application

Species

Unspecified reactive species

Naoki Nakagawa,Jingjun Li,Keiko Yabuno-Nakagawa,Tae-Yeon Eom,Martis Cowles,Tavien Mapp,Robin Taylor,E S Anton

Molecular biology of the cell 28:1088-1100 PubMed28228550

2017

Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition.

Applications

Unspecified application

Species

Unspecified reactive species

Lynne A Lapierre,Elizabeth H Manning,Kenya M Mitchell,Cathy M Caldwell,James R Goldenring

Cell 167:961-972.e16 PubMed27773481

2016

Distinct Hippocampal Pathways Mediate Dissociable Roles of Context in Memory Retrieval.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Xu,Sabine Krabbe,Jan Gründemann,Paolo Botta,Jonathan P Fadok,Fumitaka Osakada,Dieter Saur,Benjamin F Grewe,Mark J Schnitzer,Edward M Callaway,Andreas Lüthi

PloS one 9:e115095 PubMed25493438

2014

A Quantitative Approach to Evaluate the Impact of Fluorescent Labeling on Membrane-Bound HIV-Gag Assembly by Titration of Unlabeled Proteins.

Applications

WB

Species

Unspecified reactive species

Julia Gunzenhäuser,Romain Wyss,Suliana Manley
View all publications
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