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Anti-RFP rabbit polyclonal antibody that is used to detect RFP in Western blot.

- Specifically recognizes RFP and has been shown to react with tdTomato
- Cited in over 320 publications
- Trusted since 2008


Images

Western blot - Anti-RFP antibody (AB62341), expandable thumbnail
  • Western blot - Anti-RFP antibody (AB62341), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.146% EDTA

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Sea anenome RFP_DISSP. Database link Q9U6Y8

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Tag
Tested

Tested
Tested

Species
Tag
Dilution info
0.5-4 µg/mL
Notes

-

Associated Products

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Target data

Function

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.

Alternative names

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Anti-RFP rabbit polyclonal antibody that is used to detect RFP in Western blot.

- Specifically recognizes RFP and has been shown to react with tdTomato
- Cited in over 320 publications
- Trusted since 2008

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Sea anenome RFP_DISSP. Database link Q9U6Y8
Purification technique
Affinity purification Immunogen
Specificity

ab62341 recognizes RFP and has been shown to react with tdTomato.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is manufactured by BioVision, an Abcam company and was previously called 3993 RFP (dsRed) Antibody. 3993-100 is the same size as the 100 µg size of ab62341.

Product Specifications
Anti-RFP antibody (ab62341) is a rabbit polyclonal antibody and is validated for use in WB.
Anti-RFP antibody (ab62341) specifically detects RFP (UniProt ID: Q9U6Y8; Molecular weight: 26kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-RFP antibody (ab62341) has high sensitivity and specificity.
Anti-RFP antibody (ab62341) has been cited over 320 times in peer reviewed journals and is trusted by the scientific community.
Anti-RFP antibody (ab62341) has 22 independent reviews from customers.

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Conjugation-ready, carrier free format available for antibody clone - Anti-Smad4 antibody [EP618Y] - BSA and Azide free ab215968.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-RFP antibody (ab62341), expandable thumbnail

    Western blot - Anti-RFP antibody (ab62341)

    Blocking/Dilution buffer: 5% NFDM (1 hr at RT) / TBST

    The protein runs larger on a SDS PAGE gel.

    All lanes: Western blot - Anti-RFP antibody (ab62341) at 1/1000 dilution

    Lane 1: Recombinant RFP protein at 0.01 µg

    Lane 2: Recombinant RFP protein at 0.05 µg

    Lane 3: Recombinant RFP protein at 0.25 µg

    Lane 4: Recombinant RFP protein at 1 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG peroxidase conjugate at 1/10000 dilution

    Predicted band size: 26 kDa

    Observed band size: 32 kDa

  • Western blot - Anti-RFP antibody (ab62341), expandable thumbnail

    Western blot - Anti-RFP antibody (ab62341)

    Blocking Buffer - 3% Milk

    All lanes: Western blot - Anti-RFP antibody (ab62341) at 1 µg/mL

    Lane 1: Recombinant RFP at 2 ng

    Lane 2: Recombinant RFP at 10 ng

    Lane 3: Recombinant RFP at 50 ng

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Observed band size: 32 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com