Anti-RFP antibody

• WB

Supplier Data

Western blot - Anti-RFP antibody (AB62341)

Blocking/Dilution buffer : 5% NFDM (1 hr at RT) / TBST

The protein runs larger on a SDS PAGE gel.

All lanes:

Western blot - Anti-RFP antibody (ab62341) at 1/1000 dilution

Lane 1:

Recombinant RFP protein at 0.01 µg

Lane 2:

Recombinant RFP protein at 0.05 µg

Lane 3:

Recombinant RFP protein at 0.25 µg

Lane 4:

Recombinant RFP protein at 1 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG peroxidase conjugate at 1/10000 dilution

Predicted band size: 26 kDa

Observed band size: 32 kDa

false

• WB

Supplier Data

Western blot - Anti-RFP antibody (AB62341)

Blocking Buffer - 3% Milk

All lanes:

Western blot - Anti-RFP antibody (ab62341) at 1 µg/mL

Lane 1:

Recombinant RFP at 2 ng

Lane 2:

Recombinant RFP at 10 ng

Lane 3:

Recombinant RFP at 50 ng

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Observed band size: 32 kDa

false

• WB

CiteAb

Western blot - Anti-RFP antibody (AB62341)

RFP western blotting using Anti-RFP antibody ab62341. Publication image and figure legend from Cacicedo, M. L., Weinl-Tenbruck, C., et al., 2022, Front Bioeng Biotechnol, PubMed 36277393.

Cre mRNA-LNPs injected IV into CRE reporter mice elicits reporter gene activation in the liver. (A) Schematic representation of the reporter mouse strain, B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (CRE-mouse). (B) CRE mice were injected IV and euthanized 24 h later. Ex vivo imaging of tissues are shown for : Group 1, CRE mice injected with Cre mRNA-LNPs; Group 2, CRE mice injected with NaCl; Group 3, C57BL/6J mice injected with Cre mRNA-LNPs; Group 4, C57BL/6J mice injected with NaCl. (C) Quantification of tdTomato fluorescent signal obtained for livers after ex vivo imaging. (D) Western blot analysis of tdTomato protein (visualized by anti-RFP antibody; RFP, Red Fluorescent Protein) in CRE mouse livers normalized to β-actin loading control. (E) Quantitation of tdTomato protein bands normalized to β-actin loading control. Results are the means ± SEM for groups of mice (n = 5) injected with Cre mRNA-LNPs or saline. Statistically significant difference to the saline control : ****p < < 0.0001 (two-tailed Student's t-test).

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