Anti-RFP rabbit polyclonal antibody that is used to detect RFP in Western blot.
- Specifically recognizes RFP and has been shown to react with tdTomato
- Cited in over 320 publications
- Trusted since 2008
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.146% EDTA
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Tag | Tested |
Species | Dilution info | Notes |
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Species Tag | Dilution info 0.5-4 µg/mL | Notes - |
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Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.
Red fluorescent protein drFP583, DsRed
Anti-RFP rabbit polyclonal antibody that is used to detect RFP in Western blot.
- Specifically recognizes RFP and has been shown to react with tdTomato
- Cited in over 320 publications
- Trusted since 2008
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.146% EDTA
ab62341 recognizes RFP and has been shown to react with tdTomato.
This product is manufactured by BioVision, an Abcam company and was previously called 3993 RFP (dsRed) Antibody. 3993-100 is the same size as the 100 µg size of ab62341.
Product SpecificationsAbcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking/Dilution buffer: 5% NFDM (1 hr at RT) / TBST
The protein runs larger on a SDS PAGE gel.
All lanes: Western blot - Anti-RFP antibody (ab62341) at 1/1000 dilution
Lane 1: Recombinant RFP protein at 0.01 µg
Lane 2: Recombinant RFP protein at 0.05 µg
Lane 3: Recombinant RFP protein at 0.25 µg
Lane 4: Recombinant RFP protein at 1 µg
All lanes: Goat Anti-Rabbit IgG peroxidase conjugate at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 32 kDa
Blocking Buffer - 3% Milk
All lanes: Western blot - Anti-RFP antibody (ab62341) at 1 µg/mL
Lane 1: Recombinant RFP at 2 ng
Lane 2: Recombinant RFP at 10 ng
Lane 3: Recombinant RFP at 50 ng
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Observed band size: 32 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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