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Mouse Monoclonal RFP antibody. Suitable for WB and reacts with Tag samples. Cited in 12 publications.


Images

Western blot - Anti-RFP antibody [RF5R] (AB125244), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Tag
Tested

Tested
Tested

Species
Tag
Dilution info
1/1000 - 1/3000
Notes

Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins.

Associated Products

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9 products for Alternative Product

Target data

Function

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.

Alternative names

Red fluorescent protein drFP583, DsRed

Recommended products

Mouse Monoclonal RFP antibody. Suitable for WB and reacts with Tag samples. Cited in 12 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
RF5R
Purification technique
Affinity purification Protein A
Specificity

Recognizes native and denatured forms of RFP and its turbo-RFP, DsRed variants

Concentration
Loading...
Purification notes

ab125244 is purified by protein A affinity chromatography from TCS

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Store at 4°C (add 0.1% NaN3) for several days to weeks.

This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-RFP antibody [RF5R] (ab125244), expandable thumbnail

    Western blot - Anti-RFP antibody [RF5R] (ab125244)

    All lanes: Western blot - Anti-RFP antibody [RF5R] (ab125244) at 1/1000 dilution

    Lane 1: HEK293 cell lysate, non-transfected

    Lane 2: HEK293 cell lysate, Turbo-RFP-transfected

    Lane 3: HEK293 cell lysate, DsRed-transfected

    Developed using the ECL technique.

    Predicted band size: 26 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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