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AB125244

Anti-RFP antibody [RF5R]

0

(1 Review)

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(15 Publications)

Mouse Monoclonal RFP antibody. Suitable for WB and reacts with Tag samples. Cited in 15 publications.

View Alternative Names

Red fluorescent protein drFP583, DsRed

1 Images
Western blot - Anti-RFP antibody [RF5R] (AB125244)
  • WB

Unknown

Western blot - Anti-RFP antibody [RF5R] (AB125244)

All lanes:

Western blot - Anti-RFP antibody [RF5R] (ab125244) at 1/1000 dilution

Lane 1:

HEK293 cell lysate, non-transfected

Lane 2:

HEK293 cell lysate, Turbo-RFP-transfected

Lane 3:

HEK293 cell lysate, DsRed-transfected

Predicted band size: 26 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RF5R

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recognizes native and denatured forms of RFP and its turbo-RFP, DsRed variants

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p>Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins.</p>" } } }
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Product details

Store at 4°C (add 0.1% NaN3) for several days to weeks.

This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab125244 is purified by protein A affinity chromatography from TCS
Storage buffer
pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.
See full target information Red fluorescent protein drFP583
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112845 PubMed40612507

2025

Cellular heterogeneity and patterning strategies as revealed by upper respiratory epithelium single cell atlas.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander G Foote,Xin Sun

Nature communications 15:7145 PubMed39164270

2024

Lgr5 marks stem/progenitor cells contributing to epithelial and muscle development in the mouse esophagus.

Applications

Unspecified application

Species

Unspecified reactive species

Lana Kostic,Carly Leung,Katzrin Ahmad Murad,Snezhina Kancheva,Stefano Perna,Bernett Lee,Nick Barker

Nature communications 14:1768 PubMed36997511

2023

The plant unique ESCRT component FREE1 regulates autophagosome closure.

Applications

Unspecified application

Species

Unspecified reactive species

Yonglun Zeng,Baiying Li,Shuxian Huang,Hongbo Li,Wenhan Cao,Yixuan Chen,Guoyong Liu,Zhenping Li,Chao Yang,Lei Feng,Jiayang Gao,Sze Wan Lo,Jierui Zhao,Jinbo Shen,Yan Guo,Caiji Gao,Yasin Dagdas,Liwen Jiang

Cell reports 37:109975 PubMed34758317

2021

Sox6 expression distinguishes dorsally and ventrally biased dopamine neurons in the substantia nigra with distinctive properties and embryonic origins.

Applications

Unspecified application

Species

Unspecified reactive species

Milagros Pereira Luppi,Maite Azcorra,Giuliana Caronia-Brown,Jean-Francois Poulin,Zachary Gaertner,Serafin Gatica,Oscar Andrés Moreno-Ramos,Navid Nouri,Marilyn Dubois,Yongchao C Ma,Charu Ramakrishnan,Lief Fenno,Yoon Seok Kim,Karl Deisseroth,Francesca Cicchetti,Daniel A Dombeck,Rajeshwar Awatramani

Journal of cellular biochemistry 122:1848-1862 PubMed34431132

2021

Miro1 functions as an inhibitory regulator of MFN at elevated mitochondrial Ca levels.

Applications

Unspecified application

Species

Unspecified reactive species

Ferdinand F Fatiga,Li-Jie Wang,Tian Hsu,Jenica Irish Capuno,Chi-Yu Fu

Molecular brain 14:67 PubMed33845857

2021

Loss of Calretinin in L5a impairs the formation of the barrel cortex leading to abnormal whisker-mediated behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhao Su,Junhua Liu,Baocong Yu,Kaixing Zhou,Congli Sun,Mengjie Yang,Chunjie Zhao

Cell reports 33:108535 PubMed33357435

2020

Lgr5 Marks Adult Progenitor Cells Contributing to Skeletal Muscle Regeneration and Sarcoma Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Carly Leung,Katzrin Bte Ahmad Murad,Adelyn Liang Thing Tan,Swathi Yada,Sowmya Sagiraju,Peter Karl Bode,Nick Barker

American journal of nuclear medicine and molecular 9:321-334 PubMed31976162

2019

Dynamic monitoring of STAT3 activation in live cells using a novel STAT3 Phospho-BRET sensor.

Applications

Unspecified application

Species

Unspecified reactive species

Shalini Dimri,Rohit Arora,Akshi Jasani,Abhijit De

Journal of virology 93: PubMed31578292

2019

Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State.

Applications

Unspecified application

Species

Unspecified reactive species

Tonya Kueck,Louis-Marie Bloyet,Elena Cassella,Trinity Zang,Fabian Schmidt,Vesna Brusic,Gergely Tekes,Owen Pornillos,Sean P J Whelan,Paul D Bieniasz

Nature communications 10:5378 PubMed31772170

2019

Neonatal Wnt-dependent Lgr5 positive stem cells are essential for uterine gland development.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Seishima,Carly Leung,Swathi Yada,Katzrin Bte Ahmed Murad,Liang Thing Tan,Amin Hajamohideen,Si Hui Tan,Hideki Itoh,Kazuhiro Murakami,Yoshihiro Ishida,Satoshi Nakamizo,Yusuke Yoshikawa,Esther Wong,Nick Barker
View all publications
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Product promise

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