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AB122738

Anti-RFRP antibody

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(3 Publications )

Rabbit Polyclonal RFRP antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NPVF aa 100-200.
1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFRP antibody (AB122738)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFRP antibody (AB122738)

Immunohistochemical analysis of human retina tissue labeling RFRP with ab122738 at a 1/1000 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NPVF aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q9HCQ7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RFRP also known as RFamide-related peptide ranges in mass from 1.38 kDa to 2.85 kDa depending on the specific peptide variant. RFRP is expressed in the central nervous system particularly in the hypothalamus and pituitary gland and also found within the testis and ovary tissues. It originates from the VGF peptide precursor and plays a role in modulating various physiological processes.
Biological function summary

The RFamide-related peptide influences reproductive functions such as the regulation of gonadotropin release. It functions as an inhibitory factor on the secretion of gonadotropin-releasing hormone (GnRH) working in tandem with other neuropeptides and factors. RFRP is not a part of a larger complex but interacts closely with related neurohormones to exert its physiological effects.

Pathways

Several neuroendocrine control systems involve the RFamide-related peptide. RFRP plays an important role in the hypothalamic-pituitary-gonadal (HPG) axis where it modulates the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It interacts with receptor proteins like GPR147 which influences these essential pathways and subsequently affects reproductive system functionality.

The RFamide-related peptide has links to reproductive health issues such as delayed puberty and infertility. Abnormal activities or levels of RFRP can interfere with hormonal balance making it a potential target for addressing these reproductive system disorders. Additionally its regulation of GnRH suggests possible connections to disorders involving altered hormonal cycles including polycystic ovary syndrome (PCOS) where excessive or insufficient RFRP activity can disrupt cyclical hormone release patterns.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neuropeptide RFRP-1. Efficiently inhibits forskolin-induced production of cAMP. Acts as a potent negative regulator of gonadotropin synthesis and secretion (PubMed : 11025660, PubMed : 20027225). Induces secretion of prolactin (By similarity).. Neuropeptide NPVF. Efficiently inhibits forskolin-induced production of cAMP. Blocks morphine-induced analgesia.. Neuropeptide RFRP-2. Shows no inhibitory activity of forskolin-induced production of cAMP.
See full target information NPVF

Alternative Names

C7orf9, RFRP, NPVF, Pro-FMRFamide-related neuropeptide VF
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Publications (3)

Recent publications for all applications. Explore the full listand refine your search

Annals of neurology 89:402-407 PubMed33085104

2020

Progressive Myoclonus Epilepsy Caused by a Homozygous Splicing Variant of SLC7A6OS.

Applications

Unspecified application

Species

Unspecified reactive species

Laure Mazzola,Karen L Oliver,Audrey Labalme,Betül Baykan,Mikko Muona,Tarja H Joensuu,Carolina Courage,Nicolas Chatron,Giuseppe Borsani,Eudeline Alix,Francis Ramond,Renaud Touraine,Melanie Bahlo,Nerses Bebek,Samuel F Berkovic,Anna-Elina Lehesjoki,Gaetan Lesca

Science advances 5:eaax4249 PubMed31763451

2019

Evolutionarily conserved regulation of sleep by epidermal growth factor receptor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel A Lee,Justin Liu,Young Hong,Jacqueline M Lane,Andrew J Hill,Sarah L Hou,Heming Wang,Grigorios Oikonomou,Uyen Pham,Jae Engle,Richa Saxena,David A Prober

Journal of neuroendocrinology 29: PubMed28295740

2017

Green light inhibits GnRH-I expression by stimulating the melatonin-GnIH pathway in the chick brain.

Applications

Unspecified application

Species

Unspecified reactive species

L Zhang,F Chen,J Cao,Y Dong,Z Wang,M Hu,Y Chen
View all publications
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Product promise

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