Guinea pig Polyclonal RFRP antibody. Suitable for IHC-Fr and reacts with Sheep samples. Immunogen corresponding to Synthetic Peptide within Human NPVF aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Guinea pig
- Storage buffer
pH: 7 - 7.4
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NPVF aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCQ7
Reactivity data
Select an application| IHC-Fr | |
|---|---|
| Human | Predicted |
| Sheep | Tested |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep | Dilution info 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Neuropeptide RFRP-1. Efficiently inhibits forskolin-induced production of cAMP. Acts as a potent negative regulator of gonadotropin synthesis and secretion (PubMed:11025660, PubMed:20027225). Induces secretion of prolactin (By similarity). Neuropeptide NPVF. Efficiently inhibits forskolin-induced production of cAMP. Blocks morphine-induced analgesia. Neuropeptide RFRP-2. Shows no inhibitory activity of forskolin-induced production of cAMP.
Alternative names
C7orf9, RFRP, NPVF, Pro-FMRFamide-related neuropeptide VF
Recommended products
Guinea pig Polyclonal RFRP antibody. Suitable for IHC-Fr and reacts with Sheep samples. Immunogen corresponding to Synthetic Peptide within Human NPVF aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Guinea pig
- Storage buffer
pH: 7 - 7.4
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NPVF aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCQ7
- Purity
- Whole antiserum
- Reconstitution
- Reconstitute in 50 µl sterile water. Centrifuge to remove any insoluble material. After reconstitution, aliquot and store at -20°C for a higher stability.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RFRP also known as RFamide-related peptide ranges in mass from 1.38 kDa to 2.85 kDa depending on the specific peptide variant. RFRP is expressed in the central nervous system particularly in the hypothalamus and pituitary gland and also found within the testis and ovary tissues. It originates from the VGF peptide precursor and plays a role in modulating various physiological processes.
- Biological function summary
The RFamide-related peptide influences reproductive functions such as the regulation of gonadotropin release. It functions as an inhibitory factor on the secretion of gonadotropin-releasing hormone (GnRH) working in tandem with other neuropeptides and factors. RFRP is not a part of a larger complex but interacts closely with related neurohormones to exert its physiological effects.
- Pathways
Several neuroendocrine control systems involve the RFamide-related peptide. RFRP plays an important role in the hypothalamic-pituitary-gonadal (HPG) axis where it modulates the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It interacts with receptor proteins like GPR147 which influences these essential pathways and subsequently affects reproductive system functionality.
- Associated diseases and disorders
The RFamide-related peptide has links to reproductive health issues such as delayed puberty and infertility. Abnormal activities or levels of RFRP can interfere with hormonal balance making it a potential target for addressing these reproductive system disorders. Additionally its regulation of GnRH suggests possible connections to disorders involving altered hormonal cycles including polycystic ovary syndrome (PCOS) where excessive or insufficient RFRP activity can disrupt cyclical hormone release patterns.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (ab228496)
4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis.
Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (ab228496)
4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with Kisspeptin.
Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (ab228496)
4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with Prolactin-releasing peptide (PrRP).
Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (ab228496)
4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with NFF peptide.
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