AB228496

Anti-RFRP antibody

Name: Anti-RFRP antibody (ab228496) | Abcam
Brand: Abcam Limited
SKU: AB228496

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(2 Publications)

Guinea pig Polyclonal RFRP antibody. Suitable for IHC-Fr and reacts with Sheep samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NPVF aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

C7orf9, RFRP, NPVF, Pro-FMRFamide-related neuropeptide VF

4 Images

Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (AB228496)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (AB228496)

4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with Prolactin-releasing peptide (PrRP).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (AB228496)

4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with Kisspeptin.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (AB228496)

4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with NFF peptide.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (AB228496)

4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis.

Key facts

Host species

Guinea pig

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Sheep

Applications

IHC-Fr

applications

Immunogen

Synthetic Peptide within Human NPVF aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9HCQ7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Sheep": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/2000", "IHCFr-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 50µL

Purity

Whole antiserum

Storage buffer

pH: 7 - 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RFRP also known as RFamide-related peptide ranges in mass from 1.38 kDa to 2.85 kDa depending on the specific peptide variant. RFRP is expressed in the central nervous system particularly in the hypothalamus and pituitary gland and also found within the testis and ovary tissues. It originates from the VGF peptide precursor and plays a role in modulating various physiological processes.

Biological function summary

The RFamide-related peptide influences reproductive functions such as the regulation of gonadotropin release. It functions as an inhibitory factor on the secretion of gonadotropin-releasing hormone (GnRH) working in tandem with other neuropeptides and factors. RFRP is not a part of a larger complex but interacts closely with related neurohormones to exert its physiological effects.

Pathways

Several neuroendocrine control systems involve the RFamide-related peptide. RFRP plays an important role in the hypothalamic-pituitary-gonadal (HPG) axis where it modulates the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It interacts with receptor proteins like GPR147 which influences these essential pathways and subsequently affects reproductive system functionality.

The RFamide-related peptide has links to reproductive health issues such as delayed puberty and infertility. Abnormal activities or levels of RFRP can interfere with hormonal balance making it a potential target for addressing these reproductive system disorders. Additionally its regulation of GnRH suggests possible connections to disorders involving altered hormonal cycles including polycystic ovary syndrome (PCOS) where excessive or insufficient RFRP activity can disrupt cyclical hormone release patterns.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Neuropeptide RFRP-1. Efficiently inhibits forskolin-induced production of cAMP. Acts as a potent negative regulator of gonadotropin synthesis and secretion (PubMed : 11025660, PubMed : 20027225). Induces secretion of prolactin (By similarity).. Neuropeptide NPVF. Efficiently inhibits forskolin-induced production of cAMP. Blocks morphine-induced analgesia.. Neuropeptide RFRP-2. Shows no inhibitory activity of forskolin-induced production of cAMP.

See full target information NPVF

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39122556

2024

Single-Cell Profiling Uncovers Evolutionary Divergence of Hypocretin/Orexin Neuronal Subpopulations.

Applications

Unspecified application

Species

Unspecified reactive species

Dana Sagi,Muhammad Tibi,Inbal Admati,Tali Lerer-Goldshtein,Hannah Hochgerner,Amit Zeisel,Lior Appelbaum

Nutrients 13: PubMed34684588

2021

A Role for Peripheral Anandamide and 2-Arachidonoylglycerol in Short-Term Food Intake and Orexigenic Hypothalamic Responses in a Species with Continuous Nutrient Delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel van Ackern,Angela Kuhla,Björn Kuhla

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RFRP antibody

Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (AB228496)

Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (AB228496)

Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (AB228496)

Immunohistochemistry (Frozen sections) - Anti-RFRP antibody (AB228496)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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