AB138030

Anti-RFWD3 antibody

Name: Anti-RFWD3 antibody (ab138030) | Abcam
Brand: Abcam Limited
SKU: AB138030
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal RFWD3 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human RFWD3.

View Alternative Names

RNF201, RFWD3, E3 ubiquitin-protein ligase RFWD3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human RFWD3. The exact immunogen used to generate this antibody is proprietary information.

Q6PCD5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab138030 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RFWD3 also known as RNF201 is an E3 ubiquitin-protein ligase with a mass of approximately 75 kDa. This protein adds ubiquitin to specific substrate proteins marking them for degradation by the proteasome. RFWD3 is mainly expressed in the cell nucleus where it plays a role in maintaining genomic stability. The ubiquitination process mediated by RFWD3 is critical for its function in DNA repair mechanisms especially in response to DNA damage.

Biological function summary

RFWD3 is key in the context of DNA repair and cell cycle regulation. It acts in conjunction with other repair proteins forming complexes necessary for homologous recombination repair. This protein interacts with replication protein A (RPA) during DNA damage response facilitating repair by driving proper assembly and disassembly of DNA repair complexes. RFWD3 ensures the preservation of genomic fidelity and cell viability under conditions of stress.

Pathways

RFWD3 plays a central role in DNA damage response and repair mechanisms. It is directly involved in the homologous recombination pathway working alongside proteins such as CHK1 and BRCA1. RFWD3 contributes to the stability of these pathways enabling error-free repair of DNA double-strand breaks. The protein's function in these pathways is critical for the prevention of chromosomal aberrations and cancerous transformations.

RFWD3 has been linked to cancer and Fanconi anemia. Defects in RFWD3 can lead to impaired DNA repair processes resulting in genomic instability and promoting carcinogenesis. Additionally it plays a role in the Fanconi anemia pathway interacting with proteins like FANCD2 and FANCI. This interaction is essential for proper DNA crosslink repair and deficiencies can contribute to the development of Fanconi anemia characterized by bone marrow failure and increased cancer risk.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed : 21504906, PubMed : 21558276, PubMed : 26474068, PubMed : 28575657, PubMed : 28575658, PubMed : 33321094). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed : 21504906, PubMed : 21558276, PubMed : 26474068, PubMed : 28575657, PubMed : 28575658, PubMed : 28691929). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed : 26474068, PubMed : 28575657, PubMed : 28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed : 20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed : 20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed : 20173098). Required to translesion DNA synthesis across DNA-protein cross-link adducts by catalyzing ubiquitination of proteins on single-stranded DNA (ssDNA) (PubMed : 33321094).

See full target information RFWD3

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 14:1389472 PubMed38711848

2024

RFWD3 modulates response to platinum chemotherapy and promotes cancer associated phenotypes in high grade serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah J Taylor,Robert L Hollis,Charlie Gourley,C Simon Herrington,Simon P Langdon,Mark J Arends

Breast cancer : basic and clinical research 18:11782234241234771 PubMed38504674

2024

MicroRNA-190b Targets RFWD3 in Estrogen Receptor-Positive Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabet Alexandra Frick,Karen Kristjansdottir,Snaedís Ragnarsdottir,Arnar Ingi Vilhjalmsson,Maria Rose Bustos,Linda Vidarsdottir,Thorkell Gudjonsson,Stefan Sigurdsson

Biomolecules 12: PubMed35454069

2022

Targeted Degradation of 53BP1 Using Ubiquitin Variant Induced Proximity.

Applications

Unspecified application

Species

Unspecified reactive species

Bayonle Aminu,Julia Fux,Evan Mallette,Nathaniel Petersen,Wei Zhang

Molecular cell 81:442-458.e9 PubMed33321094

2020

The ubiquitin ligase RFWD3 is required for translesion DNA synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Gallina,Ivo A Hendriks,Saskia Hoffmann,Nicolai B Larsen,Joachim Johansen,Camilla S Colding-Christensen,Lisa Schubert,Selene Sellés-Baiget,Zita Fábián,Ulrike Kühbacher,Alan O Gao,Markus Räschle,Simon Rasmussen,Michael L Nielsen,Niels Mailand,Julien P Duxin

Cell cycle (Georgetown, Tex.) :1-12 PubMed33044890

2020

The E3 ligase RFWD3 stabilizes ORC in a p53-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Rosaline Y C Hsu,Sumanprava Giri,Yating Wang,Yo-Chuen Lin,Dazhen Liu,Susan Wopat,Arindam Chakraborty,Kannanganattu V Prasanth,Supriya G Prasanth

Molecular cell 66:622-634.e8 PubMed28575658

2017

RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination.

Applications

Unspecified application

Species

Unspecified reactive species

Shojiro Inano,Koichi Sato,Yoko Katsuki,Wataru Kobayashi,Hiroki Tanaka,Kazuhiro Nakajima,Shinichiro Nakada,Hiroyuki Miyoshi,Kerstin Knies,Akifumi Takaori-Kondo,Detlev Schindler,Masamichi Ishiai,Hitoshi Kurumizaka,Minoru Takata

Molecular cell 66:610-621.e4 PubMed28575657

2017

RPA-Mediated Recruitment of the E3 Ligase RFWD3 Is Vital for Interstrand Crosslink Repair and Human Health.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Feeney,Ivan M Muñoz,Christophe Lachaud,Rachel Toth,Paul L Appleton,Detlev Schindler,John Rouse

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RFWD3 antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise