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AB99306

Anti-RFWD3 antibody

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(3 Publications)

Rabbit Polyclonal RFWD3 antibody. Suitable for IP and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RFWD3 aa 50-150.

View Alternative Names

RNF201, RFWD3, E3 ubiquitin-protein ligase RFWD3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201

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Immunoprecipitation - Anti-RFWD3 antibody (AB99306)
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Immunoprecipitation - Anti-RFWD3 antibody (AB99306)

An anti RFWD3 antibody, at 1 µg/ml, staining RFWD3 in HeLa whole cell lysate immunoprecipitated using ab99306 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-RFWD3 antibody (ab99306)

Predicted band size: 85 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human RFWD3 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q6PCD5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RFWD3 also known as RNF201 is an E3 ubiquitin-protein ligase with a mass of approximately 75 kDa. This protein adds ubiquitin to specific substrate proteins marking them for degradation by the proteasome. RFWD3 is mainly expressed in the cell nucleus where it plays a role in maintaining genomic stability. The ubiquitination process mediated by RFWD3 is critical for its function in DNA repair mechanisms especially in response to DNA damage.
Biological function summary

RFWD3 is key in the context of DNA repair and cell cycle regulation. It acts in conjunction with other repair proteins forming complexes necessary for homologous recombination repair. This protein interacts with replication protein A (RPA) during DNA damage response facilitating repair by driving proper assembly and disassembly of DNA repair complexes. RFWD3 ensures the preservation of genomic fidelity and cell viability under conditions of stress.

Pathways

RFWD3 plays a central role in DNA damage response and repair mechanisms. It is directly involved in the homologous recombination pathway working alongside proteins such as CHK1 and BRCA1. RFWD3 contributes to the stability of these pathways enabling error-free repair of DNA double-strand breaks. The protein's function in these pathways is critical for the prevention of chromosomal aberrations and cancerous transformations.

RFWD3 has been linked to cancer and Fanconi anemia. Defects in RFWD3 can lead to impaired DNA repair processes resulting in genomic instability and promoting carcinogenesis. Additionally it plays a role in the Fanconi anemia pathway interacting with proteins like FANCD2 and FANCI. This interaction is essential for proper DNA crosslink repair and deficiencies can contribute to the development of Fanconi anemia characterized by bone marrow failure and increased cancer risk.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed : 21504906, PubMed : 21558276, PubMed : 26474068, PubMed : 28575657, PubMed : 28575658, PubMed : 33321094). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed : 21504906, PubMed : 21558276, PubMed : 26474068, PubMed : 28575657, PubMed : 28575658, PubMed : 28691929). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed : 26474068, PubMed : 28575657, PubMed : 28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed : 20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed : 20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed : 20173098). Required to translesion DNA synthesis across DNA-protein cross-link adducts by catalyzing ubiquitination of proteins on single-stranded DNA (ssDNA) (PubMed : 33321094).
See full target information RFWD3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2410937 PubMed40019400

2025

RFWD3 Reprograms Nucleotide Metabolism Through PHGDH to Induce Chemoresistance In Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenchao Zhang,Chi Yin,Lin Qi,Zhongyue Liu,Ruiling Xu,Chao Tu,Zhihong Li

Human molecular genetics 32:104-121 PubMed35925837

2022

TFIIB-related factor 1 is a nucleolar protein that promotes RNA polymerase I-directed transcription and tumour cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Wang,Qiyue Chen,Xin Wang,Shasha Zhao,Huan Deng,Baoqiang Guo,Cheng Zhang,Xiaoye Song,Wensheng Deng,Tongcun Zhang,Hongwei Ni

Scientific reports 9:14257 PubMed31582797

2019

HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Lai,Mingzhang Zhu,Wenwen Wu,Nana Rokutanda,Yukiko Togashi,Weixin Liang,Tomohiko Ohta
View all publications
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