Rabbit Polyclonal RFWD3 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RFWD3 aa 50-150.
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Publications
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- Scientific reports 9:142572019HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongqiang Lai et. al.PubMed 31582797
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RFWD3 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PCD5
Reactivity data
Select an application| IP | |
|---|---|
| Human | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:33321094). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:28691929). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098). Required to translesion DNA synthesis across DNA-protein cross-link adducts by catalyzing ubiquitination of proteins on single-stranded DNA (ssDNA) (PubMed:33321094).
Alternative names
RNF201, RFWD3, E3 ubiquitin-protein ligase RFWD3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201
Recommended products
Rabbit Polyclonal RFWD3 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RFWD3 aa 50-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RFWD3 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PCD5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RFWD3 also known as RNF201 is an E3 ubiquitin-protein ligase with a mass of approximately 75 kDa. This protein adds ubiquitin to specific substrate proteins marking them for degradation by the proteasome. RFWD3 is mainly expressed in the cell nucleus where it plays a role in maintaining genomic stability. The ubiquitination process mediated by RFWD3 is critical for its function in DNA repair mechanisms especially in response to DNA damage.
- Biological function summary
RFWD3 is key in the context of DNA repair and cell cycle regulation. It acts in conjunction with other repair proteins forming complexes necessary for homologous recombination repair. This protein interacts with replication protein A (RPA) during DNA damage response facilitating repair by driving proper assembly and disassembly of DNA repair complexes. RFWD3 ensures the preservation of genomic fidelity and cell viability under conditions of stress.
- Pathways
RFWD3 plays a central role in DNA damage response and repair mechanisms. It is directly involved in the homologous recombination pathway working alongside proteins such as CHK1 and BRCA1. RFWD3 contributes to the stability of these pathways enabling error-free repair of DNA double-strand breaks. The protein's function in these pathways is critical for the prevention of chromosomal aberrations and cancerous transformations.
- Associated diseases and disorders
RFWD3 has been linked to cancer and Fanconi anemia. Defects in RFWD3 can lead to impaired DNA repair processes resulting in genomic instability and promoting carcinogenesis. Additionally it plays a role in the Fanconi anemia pathway interacting with proteins like FANCD2 and FANCI. This interaction is essential for proper DNA crosslink repair and deficiencies can contribute to the development of Fanconi anemia characterized by bone marrow failure and increased cancer risk.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunoprecipitation - Anti-RFWD3 antibody (ab99306)
An anti RFWD3 antibody, at 1 µg/ml, staining RFWD3 in HeLa whole cell lysate immunoprecipitated using ab99306 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-RFWD3 antibody (ab99306)
Predicted band size: 85 kDa
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