Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741)

Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741) is a rabbit monoclonal antibody detecting Rfx Cas13d (N-term) in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Ruminococcus flavefaciens.

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Images

Immunoprecipitation - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (AB314741), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt (Intra)	IP
Ruminococcus flavefaciens	Predicted	Predicted	Predicted	Predicted	Predicted
Transfected cell line - Ruminococcus flavefaciens	Not recommended	Tested	Tested	Tested	Not recommended
Transfected cell lysate - Ruminococcus flavefaciens	Tested	Not recommended	Not recommended	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Ruminococcus flavefaciens	Dilution info 1/1000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Ruminococcus flavefaciens	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Ruminococcus flavefaciens	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Ruminococcus flavefaciens	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Ruminococcus flavefaciens	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Ruminococcus flavefaciens	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Ruminococcus flavefaciens	Dilution info 1/500	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Ruminococcus flavefaciens	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Ruminococcus flavefaciens	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Ruminococcus flavefaciens	Dilution info 1/500	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Ruminococcus flavefaciens	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Ruminococcus flavefaciens	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Ruminococcus flavefaciens	Dilution info 1/30	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Ruminococcus flavefaciens	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Ruminococcus flavefaciens	Dilution info -	Notes -

Target data

See full target information Rfx Cas13d

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR23965-31
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Ruminococcus flavefaciens samples.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR23965-31] also available for your convenience:
ab314741, Carrier free - ab314742

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Rfx Cas13d also known as C2c10 functions as a versatile RNA-targeted CRISPR-associated protein. Its primary mechanical action involves RNA-guided RNase activity facilitated by a guide RNA that directs the cleavage of specific RNA targets. This protein weighs approximately 930 amino acids and is expressed in various prokaryotic organisms. Engineered versions of Cas13d like Rfx Cas13d have been adapted for use in eukaryotic cells enabling targeted gene silencing and manipulation of RNA transcripts.

Biological function summary

Rfx Cas13d contributes to RNA processing and regulation playing a role in RNA interference mechanisms. It associates with RNA molecules and degrades them therefore regulating gene expression post-transcriptionally. Rfx Cas13d does not act alone; it forms part of a complex with guide RNA that determines its specificity. Its ability to cleave RNA makes it valuable for studying RNA function and interactions within cells offering insights into RNA-based regulation processes.

Pathways

Rfx Cas13d influences specific RNA regulatory pathways that control gene silencing and expression. It participates in pathways associated with RNA interference and endogenous processes similar to microRNA (miRNA) pathways. In these pathways it relates to proteins like AGO (Argonaute) which also play roles in RNA-induced silencing complexes. Through its RNA cleavage activity it helps manage RNA populations within cells ensuring correct gene expression regulation.

Associated diseases and disorders

Rfx Cas13d holds potential for therapeutic applications especially in targeting RNA viruses and genetic diseases caused by RNA dysregulation. For example its ability to degrade viral RNA makes it a prospective tool in combating viral infections such as influenza. Also it may relate to disorders involving misregulated RNA species which in some cases connect to malfunctioning proteins thereby providing potential intervention points. Cas13d's action could help counteract diseases by restoring normal RNA regulation when linked to defective proteins or pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunoprecipitation - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741)
Rfx Cas13d (N-term) was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) cells transfected with a Ruminococcus flavefaciens cas13d expression vector containing a myc-His-tag®, whole cell lysate with ab314741 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314741 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a Ruminococcus flavefaciens cas13d expression vector containing a myc-His-tag®, whole cell lysate
Lane 2: ab314741 at 1/30 IP in 293T (human embryonic kidney epithelial cell) cells transfected with a Ruminococcus flavefaciens cas13d expression vector containing a myc-His-tag®, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314741 in 293T cells transfected with a Ruminococcus flavefaciens cas13d expression vector containing a myc-His-tag®, whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
Western blot - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741)
Rfx Cas13d (N-term) Western blot staining using rabbit Anti-Rfx Cas13d (N-term) antibody
Rfx Cas13d may cleave to two fragments close to C-term, and this antibody can recognize the full length and N-terminal fragment. His-tag antibody can recognize full length and C-terminal fragment because the His-tag of this overexpression vector located on C-term.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 10 µg with 5% NFDM/TBST
Lane 2: 293T cells transfected with a Ruminococcus flavefaciens cas13d expression vector containing a myc-His-tag® (C-term), whole cell lysate at 10 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 110 kDa, 70 kDa, 124 kDa, 40 kDa
Exposure time: 6s
Western blot - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741)
Rfx Cas13d may cleave to two fragments close to C-term, and this antibody can recognize the full length and N-terminal fragment. HA tag antibody can recognize the full length and N-terminal fragment. His-tag antibody can recognize full length and C-terminal fragment because the His-tag of this overexpression vector located on C-term, HA-myc-tag of this overexpression vector located on N-term.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-HA tag antibody [EPR22819-101] - (Anti-HA tag antibody [EPR22819-101] ab236632) staining at 1/1000 dilution.
In Western blot, Anti-6XHis tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 10 µg with 5% NFDM/TBST
Lane 2: 293T cells transfected with a Ruminococcus flavefaciens cas13d expression vector containing a HA-myc-tag (N-term) and a His-tag® (C-term), whole cell lysate at 10 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 110 kDa, 70 kDa, 36 kDa, 40 kDa
Exposure time: 6s
Immunocytochemistry/ Immunofluorescence - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with a Ruminococcus flavefaciens cas13d expression vector containing a HA-myc-tag(N-term) and a His-tag®(C-term) cells labelling Rfx Cas13d (N-term) with ab314741 at 1/500 (0.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing positive staining in 293T transfected with a Ruminococcus flavefaciens cas13d expression vector containing a HA-myc-tag(N-term) and a His-tag®(C-term).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain at 1/100 (0.38ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Rfx Cas13d (N-term) with ab314741 at 1/100 (4.8 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat liver. The section was incubated with ab314741 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Rfx Cas13d (N-term) with ab314741 at 1/100 (4.8 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse liver. The section was incubated with ab314741 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Rfx Cas13d (N-term) with ab314741 at 1/100 (4.8 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human liver. The section was incubated with ab314741 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741)
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Ruminococcus flavefaciens cas13d expression vector containing a His tag. (B) HEK-293T transfected with empty vector containing a His tag. tissue labeling Rfx Cas13d (N-term) with ab314741 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Ruminococcus flavefaciens cas13d expression vector containing a His tag, negative staining on (B) HEK-293T transfected with empty vector containing a His tag. The section was incubated with ab314741 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Flow Cytometry (Intracellular) - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T transfected with a Ruminococcus flavefaciens cas13d expression vector containing a HA-myc-tag (N-term) and a His-tag® (C-term) (Middle) / 293T cells transfected with empty expression vector containing a myc-His-tag® (Right) cells labelling Rfx Cas13d (N-term) with ab314741 at 1/500 dilution (0.1 ug)/Middle and Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells are co-stained with Myc conjugated to Alexa Fluor®647.
Flow Cytometry (Intracellular) - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T transfected with a Ruminococcus flavefaciens cas13d expression vector containing a myc-His-tag® (C-term) (Middle) / 293T cells transfected with empty expression vector containing a myc-His-tag® (Right) cells labelling Rfx Cas13d (N-term) with ab314741 at 1/500 dilution (0.1 ug)/Middle and Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells are co-stained with Myc conjugated to Alexa Fluor®647.