AB168475

Anti-RFX3 antibody

Name: Anti-RFX3 antibody (ab168475) | Abcam
Brand: Abcam Limited
SKU: AB168475

Be the first to review this product! Submit a review

(1 Publication)

Mouse Polyclonal RFX3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RFX3.

View Alternative Names

Transcription factor RFX3, Regulatory factor X 3, RFX3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (AB168475)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (AB168475)

Immunofluorescent analysis of HeLa cells labeling RFX3 with ab168475 at 10 µg/ml.

Unknown

Western blot - Anti-RFX3 antibody (AB168475)

All lanes:

Western blot - Anti-RFX3 antibody (ab168475) at 1 µg/mL

Lane 1:

RFX3 transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 84 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RFX3.

P48380

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RFX3 protein also known as Regulatory Factor X 3 plays a significant role in gene transcription regulation. It has a molecular mass of approximately 83 kDa. RFX3 functions as a transcription factor binding specifically to X-box motifs within DNA influencing the expression of various genes important for cell processes. This protein is present in a variety of tissues including the brain kidney and pancreas suggesting its diverse and widespread role in cellular activities.

Biological function summary

The function of RFX3 involves the modulation of genes critical for cilia development and function. RFX3 forms part of a gene regulatory complex that oversees ciliogenesis. Through this complex it controls the transcription of genes essential for the formation of motile and primary cilia. The activity of RFX3 maintains proper cilia structure and movement essential for cellular signaling and environmental sensing.

Pathways

The involvement of RFX3 focuses largely on the ciliogenesis and hedgehog signaling pathways. RFX3 regulates genes necessary for the assembly and function of cilia linking it to the hedgehog signaling pathway where cilia play an important role in signal reception and transduction. RFX3 interacts with proteins like RFX1 and RFX2 which share similar DNA-binding domains highlighting its preserved function across these pathways.

Disruptions in RFX3 can relate to ciliopathies and diabetes. Ciliopathies such as Joubert syndrome arise from defective ciliogenesis due to compromised RFX3 function with related proteins like RFX1 also being implicated. Diabetes connects to RFX3 through its expression in pancreatic beta cells suggesting a role in insulin regulation and glucose metabolism. Changes in RFX3 levels or function can affect genes involved in these conditions indicating its importance in maintaining normal physiological processes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes (PubMed : 20148032). Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells (PubMed : 12411430).

See full target information RFX3

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 12:3743-3751 PubMed31190875

2019

Homeobox B7 accelerates the cancer progression of gastric carcinoma cells by promoting epithelial-mesenchymal transition (EMT) and activating Src-FAK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianghong Wu,Ziwen Long,Hong Cai,Shengjia Yu,Xiaowen Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RFX3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (AB168475)

Western blot - Anti-RFX3 antibody (AB168475)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise