AB92670

Anti-RFX3 antibody

Name: Anti-RFX3 antibody (ab92670) | Abcam
Brand: Abcam Limited
SKU: AB92670
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal RFX3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RFX3.

View Alternative Names

Transcription factor RFX3, Regulatory factor X 3, RFX3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX3 antibody (AB92670)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX3 antibody (AB92670)

Left panel : ab92670 at 1/50 dilution staining RFX3 in paraffin-embedded Human colon carcinoma tissue.
Right panel : same, after pre-incubation with the immunizing peptide.

Unknown

Western blot - Anti-RFX3 antibody (AB92670)

All lanes:

Western blot - Anti-RFX3 antibody (ab92670) at 1/500 dilution

Lane 1:

extracts from RAW264.7 cells at 10 µg

Lane 2:

extracts from COLO cells at 10 µg

Lane 3:

extracts from RAW264.7 cells at 10 µg with immunizing peptide

Predicted band size: 84 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human RFX3. The exact immunogen used to generate this antibody is proprietary information.

P48380

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RFX3 protein also known as Regulatory Factor X 3 plays a significant role in gene transcription regulation. It has a molecular mass of approximately 83 kDa. RFX3 functions as a transcription factor binding specifically to X-box motifs within DNA influencing the expression of various genes important for cell processes. This protein is present in a variety of tissues including the brain kidney and pancreas suggesting its diverse and widespread role in cellular activities.

Biological function summary

The function of RFX3 involves the modulation of genes critical for cilia development and function. RFX3 forms part of a gene regulatory complex that oversees ciliogenesis. Through this complex it controls the transcription of genes essential for the formation of motile and primary cilia. The activity of RFX3 maintains proper cilia structure and movement essential for cellular signaling and environmental sensing.

Pathways

The involvement of RFX3 focuses largely on the ciliogenesis and hedgehog signaling pathways. RFX3 regulates genes necessary for the assembly and function of cilia linking it to the hedgehog signaling pathway where cilia play an important role in signal reception and transduction. RFX3 interacts with proteins like RFX1 and RFX2 which share similar DNA-binding domains highlighting its preserved function across these pathways.

Disruptions in RFX3 can relate to ciliopathies and diabetes. Ciliopathies such as Joubert syndrome arise from defective ciliogenesis due to compromised RFX3 function with related proteins like RFX1 also being implicated. Diabetes connects to RFX3 through its expression in pancreatic beta cells suggesting a role in insulin regulation and glucose metabolism. Changes in RFX3 levels or function can affect genes involved in these conditions indicating its importance in maintaining normal physiological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes (PubMed : 20148032). Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells (PubMed : 12411430).

See full target information RFX3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

British journal of cancer 130:1249-1260 PubMed38361045

2024

AHRR and SFRP2 in primary versus recurrent high-grade serous ovarian carcinoma and their prognostic implication.

Applications

Unspecified application

Species

Unspecified reactive species

Nanna Monjé,Mihnea P Dragomir,Bruno V Sinn,Inga Hoffmann,Anuar Makhmut,Tincy Simon,Catarina A Kunze,Jana Ihlow,Wolfgang D Schmitt,Jonathan Pohl,Iris Piwonski,Sofya Marchenko,Carlotta Keunecke,Teodor G Calina,Francesca Tiso,Hagen Kulbe,Caroline Kreuzinger,Dan Cacsire Castillo-Tong,Jalid Sehouli,Elena I Braicu,Carsten Denkert,Silvia Darb-Esfahani,Kirsten Kübler,David Capper,Fabian Coscia,Markus Morkel,David Horst,Christine Sers,Eliane T Taube

Scientific reports 10:3173 PubMed32081887

2020

Systematic analysis of the Hippo pathway organization and oncogenic alteration in evolution.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxuan Chen,Han Han,Gayoung Seo,Rebecca Elizabeth Vargas,Bing Yang,Kimberly Chuc,Huabin Zhao,Wenqi Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RFX3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX3 antibody (AB92670)

Western blot - Anti-RFX3 antibody (AB92670)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise