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AB188020

Anti-RFX4 antibody

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(2 Publications)

Rabbit Polyclonal RFX4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RFX4 aa 500-650.

View Alternative Names

Transcription factor RFX4, Regulatory factor X 4, Testis development protein NYD-SP10, RFX4

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (AB188020)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (AB188020)

Immunohistochemical analysis of human kidney tissue labeling RFX4 with ab188020 at a 1/50 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (AB188020)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (AB188020)

Immunohistochemical analysis of human cerebral cortex tissue labeling RFX4 in the cytoplasm of neuropil with ab188020 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RFX4 antibody (AB188020)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFX4 antibody (AB188020)

Immunocytochemical analysis of AF22 (Human iPS derived Neuroepithelia stem (NES) cell line) whole cells labeling RFX4 in the nucleus with ab188020 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (AB188020)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (AB188020)

Immunohistochemical analysis of human endometrium tissue labeling RFX4 with ab188020 at a 1/50 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (AB188020)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (AB188020)

Immunohistochemical analysis of human testis tissue labeling RFX4 in the nucleus of cells in seminiferous ducts with ab188020 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RFX4 aa 500-650. The exact immunogen used to generate this antibody is proprietary information.

Q33E94

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RFX4 also known as Regulatory Factor X4 is a transcription factor that plays an important role in gene regulation. The protein has a mass of approximately 74 kDa and is expressed in the brain particularly within the central nervous system. It binds to X-box motifs influencing the transcription of various genes by forming complexes with other regulatory proteins. RFX4 is a member of the RFX transcription factor family which is characterized by a highly conserved DNA-binding domain.
Biological function summary

In the context of genetic regulation RFX4 modulates gene expression playing a role in CNS development. It is part of a transcription complex that works together with CREB binding protein and other transcriptional regulators to influence gene expression patterns. Its activity impacts cellular function by controlling genes essential for neural differentiation and development. Studies show that RFX4 is involved in the development of certain brain structures during embryonic growth.

Pathways

RFX4 contributes significantly to developmental pathways such as the Sonic Hedgehog (SHH) signaling pathway. It interacts with other proteins including GLI transcription factors to contribute to the regulation of target gene expression. RFX4 also participates in the Notch signaling pathway where it associates with other components to influence cell fate decisions during neural development. These interactions establish its role in orchestrating precise molecular processes critical for cellular differentiation and development.

Alterations in RFX4 have been linked to neurological conditions such as hydrocephalus and holoprosencephaly. These disorders often stem from defects in brain development where proper functioning of RFX4 and its interaction with proteins like SHH and CREB binding protein are impaired. Research suggests that abnormal RFX4 activity can disrupt standard pathways contributing to the pathogenesis of these diseases by influencing neural cell development and differentiation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that plays a role in early brain development. May activate transcription by interacting directly with the X-box. May activate transcription from CX3CL1 promoter through the X-box during brain development. May be required for neural tube ciliogenesis during embryogenesis (By similarity).
See full target information RFX4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:695 PubMed37872147

2023

The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangjie Wang,Yuan Xia,Yang Wang,Ruiqiu Zhu,Hongbo Li,Yu Liu,Na Shen

Communications biology 6:420 PubMed37061660

2023

Vimar/RAP1GDS1 promotes acceleration of brain aging after flies and mice reach middle age.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xiong,Qi Cheng,Yajie Li,Yanping Han,Xin Sun,Lei Liu
View all publications
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