Rabbit Polyclonal RFX4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RFX4 aa 500-650.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RFX4 aa 500-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q33E94
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Transcription factor that plays a role in early brain development. May activate transcription by interacting directly with the X-box. May activate transcription from CX3CL1 promoter through the X-box during brain development. May be required for neural tube ciliogenesis during embryogenesis (By similarity).
Alternative names
Transcription factor RFX4, Regulatory factor X 4, Testis development protein NYD-SP10, RFX4
Recommended products
Rabbit Polyclonal RFX4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RFX4 aa 500-650.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RFX4 aa 500-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q33E94
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RFX4 also known as Regulatory Factor X4 is a transcription factor that plays an important role in gene regulation. The protein has a mass of approximately 74 kDa and is expressed in the brain particularly within the central nervous system. It binds to X-box motifs influencing the transcription of various genes by forming complexes with other regulatory proteins. RFX4 is a member of the RFX transcription factor family which is characterized by a highly conserved DNA-binding domain.
- Biological function summary
In the context of genetic regulation RFX4 modulates gene expression playing a role in CNS development. It is part of a transcription complex that works together with CREB binding protein and other transcriptional regulators to influence gene expression patterns. Its activity impacts cellular function by controlling genes essential for neural differentiation and development. Studies show that RFX4 is involved in the development of certain brain structures during embryonic growth.
- Pathways
RFX4 contributes significantly to developmental pathways such as the Sonic Hedgehog (SHH) signaling pathway. It interacts with other proteins including GLI transcription factors to contribute to the regulation of target gene expression. RFX4 also participates in the Notch signaling pathway where it associates with other components to influence cell fate decisions during neural development. These interactions establish its role in orchestrating precise molecular processes critical for cellular differentiation and development.
- Associated diseases and disorders
Alterations in RFX4 have been linked to neurological conditions such as hydrocephalus and holoprosencephaly. These disorders often stem from defects in brain development where proper functioning of RFX4 and its interaction with proteins like SHH and CREB binding protein are impaired. Research suggests that abnormal RFX4 activity can disrupt standard pathways contributing to the pathogenesis of these diseases by influencing neural cell development and differentiation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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Full details and terms and conditions can be found here:
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (ab188020)
Immunohistochemical analysis of human testis tissue labeling RFX4 in the nucleus of cells in seminiferous ducts with ab188020 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-RFX4 antibody (ab188020)
Immunocytochemical analysis of AF22 (Human iPS derived Neuroepithelia stem (NES) cell line) whole cells labeling RFX4 in the nucleus with ab188020 at 2 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (ab188020)
Immunohistochemical analysis of human cerebral cortex tissue labeling RFX4 in the cytoplasm of neuropil with ab188020 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (ab188020)
Immunohistochemical analysis of human kidney tissue labeling RFX4 with ab188020 at a 1/50 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFX4 antibody (ab188020)
Immunohistochemical analysis of human endometrium tissue labeling RFX4 with ab188020 at a 1/50 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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