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AB327285

Anti-RFX5 Antibody

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Rabbit Polyclonal RFX5 antibody. Suitable for ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DNA-binding protein RFX5.

View Alternative Names

DNA-binding protein RFX5, Regulatory factor X 5, RFX5

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RFX5 Antibody (AB327285)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFX5 Antibody (AB327285)

Immunofluorescent staining of human cell line JURKAT using ab327285 at 0.25–2 µg/ml, showing localization to nucleoplasm.

ChIP - Anti-RFX5 Antibody (AB327285)
  • ChIP

Supplier Data

ChIP - Anti-RFX5 Antibody (AB327285)

ChIP-Exo-Seq composite graph for ab327285 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ChIP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DNA-binding protein RFX5.

P48382

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
See full target information DNA-binding protein RFX5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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